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dc.contributor.authorRavn-Haren, Gitte
dc.contributor.authorBugel, Susanne
dc.contributor.authorKrath, Britta N.
dc.contributor.authorHoac, Tien
dc.contributor.authorStagsted, Jan
dc.contributor.authorJorgensen, Karina
dc.contributor.authorBresson, June R.
dc.contributor.authorLarsen, Erik H.
dc.contributor.authorDragsted, Lars O.
dc.date.accessioned2009-09-25T08:30:34Z
dc.date.available2009-09-25T08:30:34Z
dc.date.issued2008-04
dc.identifier.citationBr. J. Nutr. 2008, 99 (4):883-892en
dc.identifier.issn0007-1145
dc.identifier.pmid17888202
dc.identifier.doi10.1017/S0007114507825153
dc.identifier.urihttp://hdl.handle.net/10146/82639
dc.description.abstractIncreased Se intakes have been associated with decreased risk of cancer and CVD. Several mechanisms have been proposed, including antioxidant effects through selenoproteins, induction of carcinogen metabolism and effects on the blood lipid profile. In a 4 x 1 week randomised, double-blind cross-over study, healthy young men supplemented their usual diet with selenate, Se-enriched yeast, Se-enriched milk or placebo (Se dose was 300 microg/d for selenate and Se-enriched yeast, and about 480 microg/d for Se-enriched milk) followed by 8-week washout periods. All Se sources increased serum Se levels after supplementation for 1 week. The effect of the organic forms did not differ significantly and both increased serum Se more than selenate. Conversely, thrombocyte glutathione peroxidase (GPX) was increased in the periods where subjects were supplemented with selenate but not in those where they were given Se-enriched yeast or Se-enriched milk. We found no effect on plasma lipid resistance to oxidation, total cholesterol, TAG, HDL- and LDL-cholesterol, GPX, glutathione reductase (GR) and glutathione S-transferase (GST) activities measured in erythrocytes, GPX and GR activities determined in plasma, or GR and GST activities in thrombocytes. Leucocyte expression of genes encoding selenoproteins (GPX1, TrR1 and SelP), and of electrophile response element-regulated genes (GCLC, Fra1 and NQO1) were likewise unaffected at all time points following intervention. We conclude that thrombocyte GPX is specifically increased by short-term selenate supplementation, but not by short-term supplementation with organic Se. Short-term Se supplementation does not seem to affect blood lipid markers or expression and activity of selected enzymes and a transcription factor involved in glutathione-mediated detoxification and antioxidation.
dc.language.isoenen
dc.relation.urlhttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1789632en
dc.subjectSeleniumen
dc.subjectOxidative defenceen
dc.subjectElectrophile response elementsen
dc.subjectGlutathione peroxidaseen
dc.subjectBlood lipidsen
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshAnimals
dc.subject.meshAntioxidants
dc.subject.meshBase Sequence
dc.subject.meshBiological Markers
dc.subject.meshC-Reactive Protein
dc.subject.meshCholesterol
dc.subject.meshCholesterol, HDL
dc.subject.meshCholesterol, LDL
dc.subject.meshCross-Over Studies
dc.subject.meshDNA Primers
dc.subject.meshDietary Supplements
dc.subject.meshDouble-Blind Method
dc.subject.meshFood, Fortified
dc.subject.meshGene Expression
dc.subject.meshHumans
dc.subject.meshLeukocytes
dc.subject.meshLipids
dc.subject.meshMale
dc.subject.meshMilk
dc.subject.meshMolecular Sequence Data
dc.subject.meshOxidation-Reduction
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshSelenium
dc.subject.meshYeast, Dried
dc.titleA short-term intervention trial with selenate, selenium-enriched yeast and selenium-enriched milk: effects on oxidative defence regulation.en
dc.typeArticleen
dc.identifier.journalThe British journal of nutritionen
html.description.abstractIncreased Se intakes have been associated with decreased risk of cancer and CVD. Several mechanisms have been proposed, including antioxidant effects through selenoproteins, induction of carcinogen metabolism and effects on the blood lipid profile. In a 4 x 1 week randomised, double-blind cross-over study, healthy young men supplemented their usual diet with selenate, Se-enriched yeast, Se-enriched milk or placebo (Se dose was 300 microg/d for selenate and Se-enriched yeast, and about 480 microg/d for Se-enriched milk) followed by 8-week washout periods. All Se sources increased serum Se levels after supplementation for 1 week. The effect of the organic forms did not differ significantly and both increased serum Se more than selenate. Conversely, thrombocyte glutathione peroxidase (GPX) was increased in the periods where subjects were supplemented with selenate but not in those where they were given Se-enriched yeast or Se-enriched milk. We found no effect on plasma lipid resistance to oxidation, total cholesterol, TAG, HDL- and LDL-cholesterol, GPX, glutathione reductase (GR) and glutathione S-transferase (GST) activities measured in erythrocytes, GPX and GR activities determined in plasma, or GR and GST activities in thrombocytes. Leucocyte expression of genes encoding selenoproteins (GPX1, TrR1 and SelP), and of electrophile response element-regulated genes (GCLC, Fra1 and NQO1) were likewise unaffected at all time points following intervention. We conclude that thrombocyte GPX is specifically increased by short-term selenate supplementation, but not by short-term supplementation with organic Se. Short-term Se supplementation does not seem to affect blood lipid markers or expression and activity of selected enzymes and a transcription factor involved in glutathione-mediated detoxification and antioxidation.


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