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dc.contributor.authorDanielsen, Pernille Hogh
dc.contributor.authorLoft, Steffen
dc.contributor.authorKocbach, Anette
dc.contributor.authorSchwarze, Per E.
dc.contributor.authorMoller, Peter
dc.date.accessioned2009-08-05T07:58:32Z
dc.date.available2009-08-05T07:58:32Z
dc.date.issued2009-03-31
dc.identifier.citationMutat. Res. 2009, 674 (1-2):116-122en
dc.identifier.issn0027-5107
dc.identifier.pmid19041418
dc.identifier.doi10.1016/j.mrgentox.2008.10.014
dc.identifier.urihttp://hdl.handle.net/10146/76317
dc.description.abstractGenotoxic effects of traffic-generated particulate matter (PM) are well described, whereas little data are available on PM from combustion of biomass and wood, which contributes substantially to air pollution world wide. The aim of this study was to compare the genotoxicity of wood smoke particulate matter (WSPM), authentic traffic-generated particles, mineral PM and standard reference material (SRM2975) of diesel exhaust particles in human A549 lung epithelial and THP-1 monocytic cell lines. DNA damage was measured as strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sites by the comet assay, whereas cell cytotoxicity was determined as lactate dehydrogenase release. The exposure to WSPM generated SB and FPG sites in both cell lines at concentrations from 2.5 or 25 microg/ml, which were not cytotoxic. Compared to all other studied particles, WSPM generated greater responses in terms of both SB and FPG sites. Organic extracts of WSPM and SRM2975 elicited higher levels of SB than native and washed PM at 25 and 100 microg/ml, whereas assay saturation precluded reliable assessment of FPG sites. During a 6h post-exposure period, in which the medium with PM had been replaced by fresh medium, 60% of the DNA lesions generated by WSPM were removed. In conclusion, WSPM generated more DNA damage than traffic-generated PM per unit mass in human cell lines, possibly due to the high level of polycyclic aromatic hydrocarbons in WSPM. This suggests that exposure to WSPM might be more hazardous than PM collected from vehicle exhaust with respect to development of lung cancer.
dc.description.sponsorshipPernille Høgh Danielsen, Peter Møller and Steffen Loft are partners in ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No 513943).en
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2D-4TWVX48-2&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=6c5559a5c30056e7044870c33b74a85een
dc.subjectComet assayen
dc.subjectDNA damageen
dc.subjectDNA repairen
dc.subjectOrganic extracten
dc.subjectOxidative stressen
dc.subjectStrand breaksen
dc.subjectWood smokeen
dc.subject.meshCell Line
dc.subject.meshChemical Fractionation
dc.subject.meshDNA Damage
dc.subject.meshDNA Repair
dc.subject.meshHumans
dc.subject.meshL-Lactate Dehydrogenase
dc.subject.meshOxidative Stress
dc.subject.meshParticulate Matter
dc.subject.meshSmoke
dc.subject.meshWood
dc.titleOxidative damage to DNA and repair induced by Norwegian wood smoke particles in human A549 and THP-1 cell lines.en
dc.typeArticleen
dc.identifier.journalMutation Researchen
html.description.abstractGenotoxic effects of traffic-generated particulate matter (PM) are well described, whereas little data are available on PM from combustion of biomass and wood, which contributes substantially to air pollution world wide. The aim of this study was to compare the genotoxicity of wood smoke particulate matter (WSPM), authentic traffic-generated particles, mineral PM and standard reference material (SRM2975) of diesel exhaust particles in human A549 lung epithelial and THP-1 monocytic cell lines. DNA damage was measured as strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sites by the comet assay, whereas cell cytotoxicity was determined as lactate dehydrogenase release. The exposure to WSPM generated SB and FPG sites in both cell lines at concentrations from 2.5 or 25 microg/ml, which were not cytotoxic. Compared to all other studied particles, WSPM generated greater responses in terms of both SB and FPG sites. Organic extracts of WSPM and SRM2975 elicited higher levels of SB than native and washed PM at 25 and 100 microg/ml, whereas assay saturation precluded reliable assessment of FPG sites. During a 6h post-exposure period, in which the medium with PM had been replaced by fresh medium, 60% of the DNA lesions generated by WSPM were removed. In conclusion, WSPM generated more DNA damage than traffic-generated PM per unit mass in human cell lines, possibly due to the high level of polycyclic aromatic hydrocarbons in WSPM. This suggests that exposure to WSPM might be more hazardous than PM collected from vehicle exhaust with respect to development of lung cancer.


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