Biomarkers of environmental contaminants in field population of green mussel (Perna viridis) from Karnataka-Kerala coast (South West coast of India).
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AbstractThe green mussel Perna viridis was sampled from relatively clean and contaminated sites along the Kartanata-Kerala coast (south west coast of India) to study the tissue concentration of trace metals and biological responses to stress (biomarkers) such as sister chromatid exchange (SCE), chromosomal aberration, micronucleus (MN) test, hemic neoplasia (HN), Chromotest (Ames test) and comet assay. In general, mean tissue concentrations of toxic trace metals collected from 25 sampling sites were found to be below the World Health Organisation (WHO) permissible concentration given for seafood. The digestive gland extract of mussels from all 25 sampling sites showed negative reaction for mutagenic activity (Ames test) in the absence of metabolic activation. Very low levels of chromosomal aberration, SCE, MN, HN and comet cells were observed in mussels collected from both the urban associated and relatively clean sites. This study seems to indicate that that the coastal waters of Karnataka and Kerala are minimally contaminated with genotoxic and carcinogenic chemicals.
CitationEcotoxicology 2006, 15 (4):347-352
JournalEcotoxicology (London, England)
DescriptionBiomarker: sister chromatid exchange (SCE); chromosomal aberration; micronucleus (MN); hemic neoplasia (HN), mutagenic activity; comet cells. Exposure/effect represented: exposure to trace metals (Fe, Cu, Ni, Cd, Cr, Zn, Pb, Mn) / DNA damage. Analytical technique: Toxic and essential elements in tissues were determined using AAS; metaphase chromosomes were viewed using light microscope (Olympus BX50) with image analysis facility; hemolymph was stained using the Schiff Feulgen-picromethyl blue procedure. Slides were then microscopically examined for the presence or absence of hemic neoplasia (HN); Comet assay kit (Trevigen Inc., Gaithersburg, MD, USA) was used to measure DNA damages in the digestive cells of mussels; Ames test was done using SOS-Chromotest Kit (Environmental Biodetection Products Inc., Brampton, Ontario, Canada) for genotoxicityTissue/biological material/sample size: whole tissue of mussels; gill; hemolymph; digestive cells; digestive glandRelationship with exposure or effect of interest (including dose-response): Very low levels of chromosomal aberration, SCE, MN and HN were observed in mussels collected from both the urban associated and relatively clean sites. Similarly, the % occurrence of comet cells in mussels collected from the urban associated and relatively clean sites were also in the same range. KEYWORS CLASSIFICATION: analysis;Animals;Biological Markers;biomarkers of exposure & effect: field studies;Canada;Carcinogens;Carcinogens,Environmental;chemically induced;chemistry;Chromosome Aberrations;Comet Assay;drug effects;Environmental Monitoring;field studies;Fisheries;genetics;Hematologic Neoplasms;humans;India;Industry;Micronucleus Tests;Mutagens;Perna;Research;Seawater;Sister Chromatid Exchange;toxicity;Trace Elements;Water;Water Pollutants;Water Pollutants,Chemical;World Health;