Characterization of 67 kD laminin receptor, a protein whose gene is overexpressed on treatment of cells with anti-benzo[a]pyrene-7,8-diol-9,10-epoxide.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe molecular mechanisms potentially related to tumorigenesis induced by anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (anti-BPDE) were investigated by suppression subtractive hybridization of the human bronchial epithelial cells (16HBE) carcinoma induced by BPDE-transformed 16HBE cells (16HBE-C). The 67 kD laminin receptor gene (67LR1) is one of the screened overexpressed genes in 16HBE-C cells when compared with 16HBE. In order to understand the main functions of 67LR1 gene, we amplified the full length of 67LR1 gene using reverse transcription-polymerase chain reaction (RT-PCR) method. The amplified gene products were inserted into pcDNA 3.1 Directional TOPO expression vector. We then transfected 16HBE cells with this vector and derived stable transfected 16HBE cell lines containing the 67LR1 gene by using lipofectin and G418 selection protocols. The expression products of transfected genes were analyzed by semiquantitative RT-PCR. Soft agar growth assay was carried out to identify the malignant features of 67LR1 gene. The stable transfected cell lines can form colonies in soft agar. Further, the transfected cells showed morphological changes compared to the control cells, such as the obvious pseudopods. These data suggest that the 67LR1 gene may be related to malignant transformation induced by the anti-BPDE. The 67LR1 protein may be related to the directionality of cell movement.
CitationToxicol. Sci. 2006, 90 (2):326-330
DescriptionKEYWORDS - CLASSIFICATION: 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide;Animals;biosynthesis;Base Sequence;Carcinoma,Squamous Cell;Cell Line,Tumor;Cell Transformation,Neoplastic;China;drug effects;Environmental Health;Epithelial Cells;genetics;Gene Expression Regulation,Neoplastic;Humans;Laminin;metabolism;mechanisms of carcinogenesis;Mice;Mice,Nude;Molecular Sequence Data;Public Health;Receptors,Laminin;Research;Rna;Sequence Analysis,RNA;toxicity;Transfection;Tumor Markers,Biological.
- Silencing of N-Ras gene expression using shRNA decreases transformation efficiency and tumor growth in transformed cells induced by anti-BPDE.
- Authors: Zhou L, Jiang Y, Tan A, Greenlee AR, Shen Y, Liu L, Yang Q
- Issue date: 2008 Oct
- [Construction of cell line of small hairpin RNA-mediated inhibition of HER2/neu gene expression].
- Authors: Fu J, Jiang YG, Ju Y, Shen YL, Chen XM
- Issue date: 2009 Aug
- [Study on lung carcinogenesis associated genes in human lung squamous cell carcinoma and malignant transformation of human bronchial epithelial cells induced by carcinogen].
- Authors: Lü JC, Chen JK, Ji WD, Jiang YG, Shi LY, Wu ZL, He M, Zeng BH
- Issue date: 2003 Dec 25
- TPX2 in malignantly transformed human bronchial epithelial cells by anti-benzo[a]pyrene-7,8-diol-9,10-epoxide.
- Authors: Zhang L, Huang H, Deng L, Chu M, Xu L, Fu J, Zhu Y, Zhang X, Liu S, Zhou Z, Wang Y
- Issue date: 2008 Oct 30
- [Effect of miR-542-3p on carcinogenesis induced by anti-benzo(a) pyrene-7,8-diol-9,10-epoxide].
- Authors: Zhao Y, Liu HY, Li YQ, Jiang YG
- Issue date: 2011 May