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dc.contributor.authorYu, Zhen
dc.contributor.authorMahadevan, Brinda
dc.contributor.authorLohr, Christiane V.
dc.contributor.authorFischer, Kay A.
dc.contributor.authorLouderback, Mandy A.
dc.contributor.authorKrueger, Sharon K.
dc.contributor.authorPereira, Clifford B.
dc.contributor.authorAlbershardt, Daniel J.
dc.contributor.authorBaird, William M.
dc.contributor.authorBailey, George S.
dc.contributor.authorWilliams, David E.
dc.date.accessioned2009-05-20T07:45:12Z
dc.date.available2009-05-20T07:45:12Z
dc.date.issued2006-10
dc.identifier.citationCarcinogenesis 2006, 27 (10):2116-2123en
dc.identifier.issn0143-3334
dc.identifier.pmid16704990
dc.identifier.doi10.1093/carcin/bgl072
dc.identifier.urihttp://hdl.handle.net/10146/68614
dc.descriptionDietary modulation of cancer & cancer biomarkers. Dietary item or component studied: indole-3-carbinol (I3C)Outcome studied: lung tumor multiplicity. Study type: mice resulting from reciprocal crosses between B6129 SF1/J [AHR(b-1/d)] and 129Sv/ImJ [AHR(d/d)] mice. Tissue/biological material/sample size: heart, thymus, lung, spleen, liver, kidney, abnormal lymph node, testes or ovaries, colon and skinMode of exposure: dibenzo[a,l]pyrene (DBP) was administered to pregnant mice (15 mg/kg, gavage) on gestation day 17, and 2000 p.p.m. indole-3-carbinol (I3C) was fed to half of the mice from gestation day 9 until weaning. Impact on outcome (including dose-response): Offspring born to dams fed I3C exhibited markedly fewer mortalities (P < 0.0001). Maternal dietary exposure to I3C also significantly lowered lung tumor multiplicity (P = 0.035) in offspring surviving to 10 months of age. The I3C chemoprotection was independent of either maternal or fetal AHR genotype. The bioavailability of DBP to fetal target tissue was demonstrated by assessing DNA covalent adduction with a (33)P-post-labeling assay. The bioavailability of I3C was determined by dosing a subset of pregnant mice with [(14)C]-I3C. KEYWORD CLASSIFICATION: administration & dosage;analysis;Animals;antagonists & inhibitors;Anticarcinogenic Agents;Benzopyrenes;Biological Availability;chemically induced;Diet;dietary modulation of cancer & cancer biomarkers;DNA Adducts;Female;Fetal Diseases;humans;Indoles;Liver Neoplasms,Experimental;Lung;Lung Neoplasms;Lymphoma;Male;Maternal-Fetal Exchange;metabolism;Mice;mortality;NF-kappa B;nursing;pharmacokinetics;Pregnancy;prevention & control;Research;toxicity;Toxicology;en
dc.description.abstractThe fetus and neonate are sensitive targets for chemically induced carcinogenesis. Few studies have examined the risk/benefit of chemoprotective phytochemicals, given in the maternal diet, against transplacental carcinogenesis. In this study, B6129 SF1/J (AHR(b-1/d)) and 129Sv/ImJ (AHR(d/d)) mice were cross-bred. The polycyclic aromatic hydrocarbon, dibenzo[a,l]pyrene (DBP), was administered to pregnant mice (15 mg/kg, gavage) on gestation day 17, and 2000 p.p.m. indole-3-carbinol (I3C), a chemoprotective phytochemical from cruciferous vegetables, was fed to half of the mice from gestation day 9 until weaning. Offspring born to dams fed I3C exhibited markedly fewer mortalities (P < 0.0001). Maternal dietary exposure to I3C also significantly lowered lung tumor multiplicity (P = 0.035) in offspring surviving to 10 months of age. The I3C chemoprotection was independent of either maternal or fetal AHR genotype. The bioavailability of DBP to fetal target tissue was demonstrated by assessing DNA covalent adduction with a (33)P-post-labeling assay. The bioavailability of I3C was determined by dosing a subset of pregnant mice with [(14)C]-I3C. Addition of chemoprotective agents to the maternal diet during pregnancy and nursing may be an effective new approach in reducing the incidence of cancers in children and young adults.
dc.language.isoenen
dc.relation.urlhttp://carcin.oxfordjournals.org/cgi/content/full/27/10/2116en
dc.subject.meshAnimals
dc.subject.meshAnticarcinogenic Agents
dc.subject.meshBenzopyrenes
dc.subject.meshBiological Availability
dc.subject.meshDNA Adducts
dc.subject.meshDiet
dc.subject.meshFemale
dc.subject.meshFetal Diseases
dc.subject.meshIndoles
dc.subject.meshLiver Neoplasms, Experimental
dc.subject.meshLung
dc.subject.meshLung Neoplasms
dc.subject.meshLymphoma
dc.subject.meshMale
dc.subject.meshMaternal-Fetal Exchange
dc.subject.meshMice
dc.subject.meshNF-kappa B
dc.subject.meshPregnancy
dc.titleIndole-3-carbinol in the maternal diet provides chemoprotection for the fetus against transplacental carcinogenesis by the polycyclic aromatic hydrocarbon dibenzo[a,l]pyrene.en
dc.typeArticleen
dc.identifier.journalCarcinogenesisen
html.description.abstractThe fetus and neonate are sensitive targets for chemically induced carcinogenesis. Few studies have examined the risk/benefit of chemoprotective phytochemicals, given in the maternal diet, against transplacental carcinogenesis. In this study, B6129 SF1/J (AHR(b-1/d)) and 129Sv/ImJ (AHR(d/d)) mice were cross-bred. The polycyclic aromatic hydrocarbon, dibenzo[a,l]pyrene (DBP), was administered to pregnant mice (15 mg/kg, gavage) on gestation day 17, and 2000 p.p.m. indole-3-carbinol (I3C), a chemoprotective phytochemical from cruciferous vegetables, was fed to half of the mice from gestation day 9 until weaning. Offspring born to dams fed I3C exhibited markedly fewer mortalities (P < 0.0001). Maternal dietary exposure to I3C also significantly lowered lung tumor multiplicity (P = 0.035) in offspring surviving to 10 months of age. The I3C chemoprotection was independent of either maternal or fetal AHR genotype. The bioavailability of DBP to fetal target tissue was demonstrated by assessing DNA covalent adduction with a (33)P-post-labeling assay. The bioavailability of I3C was determined by dosing a subset of pregnant mice with [(14)C]-I3C. Addition of chemoprotective agents to the maternal diet during pregnancy and nursing may be an effective new approach in reducing the incidence of cancers in children and young adults.


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