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dc.contributor.authorDysvik, Bjarte
dc.contributor.authorVasstrand, Endre N.
dc.contributor.authorLøvlie, Roger
dc.contributor.authorElgindi, Osman A-Aziz
dc.contributor.authorKross, Kenneth W.
dc.contributor.authorAarstad, Hans J.
dc.contributor.authorJohannessen, Anne Chr
dc.contributor.authorJonassen, Inge
dc.contributor.authorIbrahim, Salah O.
dc.date.accessioned2009-05-11T08:47:41Z
dc.date.available2009-05-11T08:47:41Z
dc.date.issued2006-02-15
dc.identifier.citationClin. Cancer Res. 2006, 12 (4):1109-1120en
dc.identifier.issn1078-0432
dc.identifier.pmid16489063
dc.identifier.doi10.1158/1078-0432.CCR-05-0115
dc.identifier.urihttp://hdl.handle.net/10146/67736
dc.descriptionKEYWORDS - CLASSIFICATION: analysis;African Continental Ancestry Group;Aged;Antigens,Nuclear;Carcinoma,Squamous Cell;Chemotactic Factors;Cluster Analysis;DNA-Binding Proteins;European Continental Ancestry Group;Female;Fibronectins;genetics;Gene Expression Profiling;Gene Expression Regulation,Neoplastic;Head and Neck Neoplasms;Humans;Immunohistochemistry;Keratins;Life Style;metabolism;Male;mechanisms of carcinogenesis;Middle Aged;Norway;pathology;Proteins;Research;RNA,Messenger;S100 Proteins;Sudan.en
dc.description.abstractPURPOSE: To explore possible range of gene expression profiles in head and neck squamous cell carcinomas (HNSCC) and pairwised normal controls from Sudanese (n = 72) and Norwegian (n = 45) patients using a 15K cDNA microarray and to correlate the findings with clinicopathologic variables. EXPERIMENTAL DESIGN: Samples from Sudan were grouped according to anatomic location/patients' habit of toombak (snuff) use, and 37 pools of 2 to 11 tumors matched to 37 pools of their normal controls from the same patients, respectively, were prepared. For Norway, eight pools of 3 to 11 tumors matched to eight pools of their normal controls from the same patients, respectively, were prepared according to anatomic location. Pools (n = 45) were hybridized to microarrays. For controls, 33 of the pools were hybridized against Human Reference RNA. Scanned array images were recorded, and data analysis was done in groups. For verification, results for selected genes were analyzed using quantitative real-time PCR/immunohistochemistry. RESULTS: We identified 136 genes from Sudan and 154 from Norway as differentially expressed between tumors and controls. Changes of the genes found were confirmed in >70% of the pools by hybridization against Reference RNA. Seventy-three genes and three main pathways (signal transduction, cell communication, and ligand-receptor interaction) were of relevance to the HNSCCs from both countries. Hierarchical clustering of the 73 genes identified subclasses of mixed tumors from the two populations, two independent subgroups for Norwegian tumors by their anatomic sites, and five subgroups for Sudanese tumors by their toombak habits. Quantitative real-time PCR/immunohistochemistry validated the microarray-based data. CONCLUSIONS: Differences in gene expression between tumor and nontumor tissues were identified in HNSCCs. Analysis of the two population groups revealed a common set of 73 genes within three main biological pathways. This indicates that the development of HNSCCs is mediated by similar biological pathways regardless of differences related to race, ethnicity, lifestyle, and/or exposure to environmental carcinogens. Of particular interest, however, was the valuable association of gene expression signature found with toombak use and anatomic site of the tumors.
dc.language.isoenen
dc.relation.urlhttp://clincancerres.aacrjournals.org/cgi/content/full/12/4/1109en
dc.subject.meshAfrican Continental Ancestry Group
dc.subject.meshAged
dc.subject.meshAntigens, Nuclear
dc.subject.meshCarcinoma, Squamous Cell
dc.subject.meshChemotactic Factors
dc.subject.meshCluster Analysis
dc.subject.meshDNA-Binding Proteins
dc.subject.meshEuropean Continental Ancestry Group
dc.subject.meshFemale
dc.subject.meshFibronectins
dc.subject.meshGene Expression Profiling
dc.subject.meshGene Expression Regulation, Neoplastic
dc.subject.meshHead and Neck Neoplasms
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshKeratins
dc.subject.meshLife Style
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshNorway
dc.subject.meshRNA, Messenger
dc.subject.meshS100 Proteins
dc.subject.meshSudan
dc.titleGene expression profiles of head and neck carcinomas from Sudanese and Norwegian patients reveal common biological pathways regardless of race and lifestyle.en
dc.typeArticleen
dc.identifier.journalClinical cancer research : an official journal of the American Association for Cancer Researchen
html.description.abstractPURPOSE: To explore possible range of gene expression profiles in head and neck squamous cell carcinomas (HNSCC) and pairwised normal controls from Sudanese (n = 72) and Norwegian (n = 45) patients using a 15K cDNA microarray and to correlate the findings with clinicopathologic variables. EXPERIMENTAL DESIGN: Samples from Sudan were grouped according to anatomic location/patients' habit of toombak (snuff) use, and 37 pools of 2 to 11 tumors matched to 37 pools of their normal controls from the same patients, respectively, were prepared. For Norway, eight pools of 3 to 11 tumors matched to eight pools of their normal controls from the same patients, respectively, were prepared according to anatomic location. Pools (n = 45) were hybridized to microarrays. For controls, 33 of the pools were hybridized against Human Reference RNA. Scanned array images were recorded, and data analysis was done in groups. For verification, results for selected genes were analyzed using quantitative real-time PCR/immunohistochemistry. RESULTS: We identified 136 genes from Sudan and 154 from Norway as differentially expressed between tumors and controls. Changes of the genes found were confirmed in >70% of the pools by hybridization against Reference RNA. Seventy-three genes and three main pathways (signal transduction, cell communication, and ligand-receptor interaction) were of relevance to the HNSCCs from both countries. Hierarchical clustering of the 73 genes identified subclasses of mixed tumors from the two populations, two independent subgroups for Norwegian tumors by their anatomic sites, and five subgroups for Sudanese tumors by their toombak habits. Quantitative real-time PCR/immunohistochemistry validated the microarray-based data. CONCLUSIONS: Differences in gene expression between tumor and nontumor tissues were identified in HNSCCs. Analysis of the two population groups revealed a common set of 73 genes within three main biological pathways. This indicates that the development of HNSCCs is mediated by similar biological pathways regardless of differences related to race, ethnicity, lifestyle, and/or exposure to environmental carcinogens. Of particular interest, however, was the valuable association of gene expression signature found with toombak use and anatomic site of the tumors.


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