Clara cells protein, prolactin and transcription factors of protein NF-ĸB and c-Jun/AP-1 levels in rats inhaled to stainless steel welding dust and its soluble form.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AffiliationNofer Institute of Occupational Medicine
MetadataShow full item record
AbstractWelding processes that generate fumes containing toxic metals, such as hexavalent chromium (Cr(VI)), manganese, and nickel (Ni), have been implicated in lung injury, inflammation, and lung tumor promotion in animal models. Bronchiolar epithelium Clara cells/club cells, coordinate these inflammatory responses. Clara cells secretory protein (CC16) with ant-inflammatory role. The pulmonary toxicity of welding dust (WD) was assessed for Wistar rats exposed to 60 mg/m<sup>3</sup> of respirable-size welding dust (mean diameter 1.17 μm for 1 and 2 weeks (6 h/day, 5 days/week)) or the aerosols of soluble form (SWD) in the nose-only exposure chambers. Additionally the effect of antiinflammatory betaine supplementation was assessed. Clara cells secretory protein, differential cell counts, total protein concentrations and cellular enzyme (lactate dehydrogenase - LDH) activities were determined in bronchoalveolar lavage fluid, and corticosterone and thiobarbituric acid reactive substances (TBARS) and prolactin concentrations were assessed in serum. Histopathology examination of lung, brain, liver, kidney, spleen was done. Additionally slices of brain and lung were exanimated in laser ablation inductively coupled plasma mass spectrometry. Both WD and SWD exposure evoked large bronchiolar infiltration shoved in histopathology examination. In this study, TBARS inversely correlated with a significant decrease of CC16 concentration that occurred after instillation of both WD and SWD indicating decreased anti- inflammatory potential in the lung. In WD exposed rats prolactin correlated with nuclear factor-kappa B (NF-κB), LDH, TBARS and serum levels Cr, Ni and inversely with c-Jun. In SWD exposed rats prolactin correlated with CC16 indicated effect of prolactin on the population of epithelial cells. In the current study, deleterious effects of repeated inhalation stainless steel welding dust form on club (Clara) cell secretory protein (CC16) were demonstrated. Clara cells secretory protein relation with prolactin in exposed rats to welding dust were shown and explored whether the NF-κB and c-Jun/activator protein 1 related pathway was involved. Int J Occup Med Environ Health 2018;31(5):613-632.
CitationInt J Occup Med Environ Health 2018;31(5):613–632
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial 3.0 United States
- The time-dependent health and biochemical effects in rats exposed to stainless steel welding dust and its soluble form.
- Authors: Halatek T, Stanislawska M, Kaminska I, Cieslak M, Swiercz R, Wasowicz W
- Issue date: 2017 Feb 23
- Effects of repeated Cr(VI) intratracheal instillation on club (Clara) cells and activation of nuclear factor-kappa B pathway via oxidative stress.
- Authors: Zhao L, Song Y, Pu J, Guo J, Wang Y, Chen Z, Chen T, Gu Y, Jia G
- Issue date: 2014 Nov 18
- Persistence of deposited metals in the lungs after stainless steel and mild steel welding fume inhalation in rats.
- Authors: Antonini JM, Roberts JR, Stone S, Chen BT, Schwegler-Berry D, Chapman R, Zeidler-Erdely PC, Andrews RN, Frazer DG
- Issue date: 2011 May
- Effect of short-term stainless steel welding fume inhalation exposure on lung inflammation, injury, and defense responses in rats.
- Authors: Antonini JM, Stone S, Roberts JR, Chen B, Schwegler-Berry D, Afshari AA, Frazer DG
- Issue date: 2007 Sep 15
- A comparison of cytotoxicity and oxidative stress from welding fumes generated with a new nickel-, copper-based consumable versus mild and stainless steel-based welding in RAW 264.7 mouse macrophages.
- Authors: Badding MA, Fix NR, Antonini JM, Leonard SS
- Issue date: 2014