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dc.contributor.authorLee, Kyoung-Ho
dc.contributor.authorBartsch, Helmut
dc.contributor.authorNair, Jagadeesan
dc.contributor.authorYoo, Dong-Ho
dc.contributor.authorHong, Yun-Chul
dc.contributor.authorCho, Soo-Hun
dc.contributor.authorKang, Daehee
dc.date.accessioned2009-03-26T08:45:23Z
dc.date.available2009-03-26T08:45:23Z
dc.date.issued2006-07
dc.identifier.citationCarcinogenesis 2006, 27 (7):1398-1403en
dc.identifier.issn0143-3334
dc.identifier.pmid16401636
dc.identifier.doi10.1093/carcin/bgi337
dc.identifier.urihttp://hdl.handle.net/10146/57215
dc.descriptionLifestyle modulation of cancer & cancer biomarkersLifestyle element evaluated:short-term fastingOutcome studied (cancer or cancer biomarker ): urinary biomarkers related to oxidative stress: malondialdehyde (MDA), 8-isoprostaglandin F2a (8-isoPGF), 8-hydroxydeoxyguanosine (8-OHdG) and 1,N6-ethenodeoxyadenosine (edA)Method of biomarker analysis: HPLC-UV, HPLC-fluorescence, ELISAStudy type (in vitro, animals, humans): humansStudy design (if human): cross-sectionalStudy size (if human): 52 healthy women Description of cohort(s) studied (if human):women with mean age 28, range 15-48 years old who provided urine samples both before and after the fasting program (average 7.2, range: 3-11 days).Confounders controlled for: BMIImpact on outcome: Urinary MDA levels after fasting (0.18 ± 1.10 mg/g creatinine) were lower than before fasting (0.37 ± 1.11) (n Ό 51, P < 0.01 by paired t-test) Urinary 8-isoPGF also was significantly reduced after fasting (n Ό 47, P < 0.01)The plasma leptin levels after fasting (5.89 ± 1.10 ng/ml) were significantly lower than before fasting (6.91 ± 1.13 ng/ml) (n Ό 27, P Ό 0.05 by paired t-test. KEYWORDS CLASSIFICATION: analogs & derivatives;Adolescent;Adult;blood;Chromatography,High Pressure Liquid;dietary modulation of cancer & cancer biomarkers;Deoxyadenosines;Deoxyguanosine;dietary modulation of carcinogenesis-related pathways;DNA Damage;Enzyme-Linked Immunosorbent Assay;Fasting;Female;history;Humans;Korea;Leptin;Lipid Peroxidation;Malondialdehyde;Middle Aged;Oxidative Stress;physiology;Plasma;Prostaglandins A;Research;Time Factors;urine.en
dc.description.abstractThe goal of this study was to determine whether short-term fasting changes in urinary biomarkers related to oxidative stress: malondialdehyde (MDA), 8-isoprostaglandin F2alpha (8-isoPGF), 8-hydroxydeoxy-guanosine (8-OHdG) and 1,N6-ethenodeoxyadenosine (epsilondA) among female volunteers participating in the short-term fasting program in South Korea. The study subjects were 52 healthy women (mean age 28, range 15-48 years old) who provided urine samples both before and after the fasting program (average 7.2, range: 3-11 days). Urinary MDA was measured by HPLC-UV and epsilondA levels were measured by immuno-affinity purification followed by HPLC-fluorescence detection. Urinary 8-OHdG and 8-isoPGF concentrations were determined by ELISA. Plasma leptin levels were also measured by radioimmunoassay. Information on demographic characteristics, personal habits (smoking and alcohol consumption) and previous medical history were collected by a self-administered questionnaire. Percent loss of body weight (average 6.3%, 4.28 +/- 0.25 kg) was significantly correlated with fasting duration (r = 0.70, n = 52, P < 0.01). The plasma leptin levels after fasting (5.89 +/- 1.10 ng/ml) were significantly lower than before fasting (6.91 +/- 1.13 ng/ml) (n = 27, P = 0.05). Urinary MDA levels after fasting (0.18 +/- 1.10 mg/g creatinine) were significantly lower than before fasting (0.37 +/- 1.11) (n = 51, P < 0.01). Urinary 8-isoPGF also were significantly reduced after fasting (n = 47, P < 0.01). However, there was no significant difference in 8-OHdG or epsilondA. There was a statistically significant correlation between % change of urinary MDA level with % change of 8-isoPGF level (partial correlation coefficient r = 0.57, n = 46, P = 0.01). The correlations between % change of 8-OHdG and plasma leptin was also significant (partial correlation coefficient r = 0.51, n = 27, P = 0.02). Our results demonstrate that the short-term fasting reduces lipid peroxidation products but does not affect oxidative stress-induced DNA damage.
dc.language.isoenen
dc.relation.urlhttp://carcin.oxfordjournals.org/cgi/content/full/27/7/1398en
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshChromatography, High Pressure Liquid
dc.subject.meshDNA Damage
dc.subject.meshDeoxyadenosines
dc.subject.meshDeoxyguanosine
dc.subject.meshEnzyme-Linked Immunosorbent Assay
dc.subject.meshFasting
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshLeptin
dc.subject.meshLipid Peroxidation
dc.subject.meshMalondialdehyde
dc.subject.meshMiddle Aged
dc.subject.meshOxidative Stress
dc.subject.meshProstaglandins A
dc.subject.meshTime Factors
dc.titleEffect of short-term fasting on urinary excretion of primary lipid peroxidation products and on markers of oxidative DNA damage in healthy women.en
dc.typeArticleen
dc.identifier.journalCarcinogenesisen
refterms.dateFOA2018-12-17T17:45:02Z
html.description.abstractThe goal of this study was to determine whether short-term fasting changes in urinary biomarkers related to oxidative stress: malondialdehyde (MDA), 8-isoprostaglandin F2alpha (8-isoPGF), 8-hydroxydeoxy-guanosine (8-OHdG) and 1,N6-ethenodeoxyadenosine (epsilondA) among female volunteers participating in the short-term fasting program in South Korea. The study subjects were 52 healthy women (mean age 28, range 15-48 years old) who provided urine samples both before and after the fasting program (average 7.2, range: 3-11 days). Urinary MDA was measured by HPLC-UV and epsilondA levels were measured by immuno-affinity purification followed by HPLC-fluorescence detection. Urinary 8-OHdG and 8-isoPGF concentrations were determined by ELISA. Plasma leptin levels were also measured by radioimmunoassay. Information on demographic characteristics, personal habits (smoking and alcohol consumption) and previous medical history were collected by a self-administered questionnaire. Percent loss of body weight (average 6.3%, 4.28 +/- 0.25 kg) was significantly correlated with fasting duration (r = 0.70, n = 52, P < 0.01). The plasma leptin levels after fasting (5.89 +/- 1.10 ng/ml) were significantly lower than before fasting (6.91 +/- 1.13 ng/ml) (n = 27, P = 0.05). Urinary MDA levels after fasting (0.18 +/- 1.10 mg/g creatinine) were significantly lower than before fasting (0.37 +/- 1.11) (n = 51, P < 0.01). Urinary 8-isoPGF also were significantly reduced after fasting (n = 47, P < 0.01). However, there was no significant difference in 8-OHdG or epsilondA. There was a statistically significant correlation between % change of urinary MDA level with % change of 8-isoPGF level (partial correlation coefficient r = 0.57, n = 46, P = 0.01). The correlations between % change of 8-OHdG and plasma leptin was also significant (partial correlation coefficient r = 0.51, n = 27, P = 0.02). Our results demonstrate that the short-term fasting reduces lipid peroxidation products but does not affect oxidative stress-induced DNA damage.


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