Effect of urban traffic, individual habits, and genetic polymorphisms on background urinary 1-hydroxypyrene excretion.
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Moore, Patrick S.
Ennas, Maria G.
Tocco, Maria G.
MetadataShow full item record
AbstractPURPOSE: Potential sources of exposure to polycyclic aromatic hydrocarbons (PAHs) and genetic polymorphisms were investigated in relation to their contribution to interindividual variation in baseline levels of urinary 1-hydroxypyrene (1-OHP) excretion in subjects without occupational exposure to PAHs. METHODS: Urinary excretion of 1-OHP was measured in 114 subjects, including 48 women and 66 men. Questionnaire information was collected on possible environmental and individual sources of PAH exposure. A subset of 70 individuals also was evaluated for a single-nucleotide polymorphism (Ex7+295C-->T) in the cytochrome P-450 1A2 (CYP1A2) gene, and 61 of these also were evaluated for the glutathione transferase T1 (GSTT1) gene polymorphism. RESULTS: 1-OHP values did not show a significant seasonal variability and were unaffected by age; education; body mass index; smoking status, including passive smoking; or the C-->T base substitution in position 295 of exon 7 of the CYP1A2 gene. After reciprocal adjustment with logistic regression, living in a heavily trafficked urban area (odds ratio, 4.9; 95% confidence interval, 1.0-24.9), and frequent intake of grilled meat (odds ratio, 6.9; 95% confidence interval, 1.1-43.5) were significant predictors of background urinary 1-OHP levels of 0.50 microg/g creatinine or greater. Elevated risks also were associated with daily alcohol intake greater than 65 g and the nonnull GSTT1 genotype. CONCLUSION: Our study shows that exposure to urban traffic, dietary habits, and the nonnull GSTT1 genotype may contribute to interindividual variation in background levels of 1-OHP urinary excretion in subjects without occupational exposure to PAHs.
CitationAnn Epidemiol 2007, 17 (1):1-8
JournalAnnals of epidemiology
DescriptionBiomarkers of individual susceptibility: field studiesBiomarker (including alleles if genetic): (Ex7C295C/T) in (CYP1A2) gene, GSTT1, GSTM1Effect studied (phenotype/pathology): PAH metabolismMethod of analysis: HPLC, PCRStudy design: case-controlStudy size: 114 (66 men, 48 women)Impact on outcome (including dose-response): 1-OHP urinary excretion did not vary according to the C/T base substitution in position 295 of exon 7 of the CYP1A2 gene (P=0.0004, for CYP 1A2)Men with the null GSTT1 genotype had significantly greater median levels of 1-OHP excretion (P=0.016)Correlation with other biomarkers: 1-OHPLifestyle modulation of cancer & cancer biomarkersLifestyle element evaluated: smoking, alchohol, age, BMI, grilled meatOutcome studied (cancer or cancer biomarker): 1-OHP excretionImpact on outcome: Median 1-OHP excretion did not change by smoking status No effect of ETS exposure in nonsmokers (Mann-Whitney test Z 0.61; p Z 0.54)Neither alcohol consumption nor vehicular traffic showed an association with elevated 1-OHP excretion (Mann-Whitney test: alcohol Z 1.50; p Z 0.13; vehicular traffic Z 0.96; p Z 0.34).1-OHP urinary excretion increased significantly grilled meat (Mann- Whitney testZ3.52; pZ0.0004). KEYWORDS CLASSIFICATION: analysis;Adult;Aged;Alcohol Drinking;biomarkers of exposure & effect: field studies;biomarkers of individual susceptibility: field studies;Case-Control Studies;Cytochrome P-450 CYP1A2;Diet;education;Environmental Exposure;Female;genetics;Glutathione;Glutathione Transferase;Habits;Humans;Italy;Life Style;Lymphoma;metabolism;Male;Middle Aged;Polymerase Chain Reaction;Polymorphism,Genetic;Pyrenes;Research;Risk Factors;Smoking;urine;Urban Health;Vehicle Emissions.