Protection against esophageal cancer in rodents with lyophilized berries: potential mechanisms.
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AbstractFor several years, our laboratory has been evaluating the ability of lyophilized (freeze-dried) black raspberries (Rubus occidentalis, BRBs), blackberries (R. fructicosus, BBs), and strawberries (Fragaria ananasia, STRWs) to inhibit carcinogen-induced cancer in the rodent esophagus. To assure "standardized" berry preparations for study, each berry type is of the same cultivar, picked at about the same degree of ripeness, washed and frozen within 2-4 h of the time of picking, and freeze-dried under conditions that preserve the components in the berries. Some of the known chemopreventive agents in berries include vitamins A, C, and E and folic acid; calcium and selenium; beta-carotene, alpha-carotene, and lutein; polyphenols such as ellagic acid, ferulic acid, p-coumaric acid, quercetin, and several anthocyanins; and phytosterols such as beta-sitosterol, stigmasterol, and kaempferol. In initial bioassays, freeze-dried STRW, BRB, and BB powders were mixed into AIN-76A synthetic diet at concentrations of 5% and 10% and fed to Fischer 344 rats before, during, and after treatment with the esophageal carcinogen N-nitrosomethylbenzylamine (NMBA). At 25 wk of the bioassay, all three berry types were found to inhibit the number of esophageal tumors (papillomas) in NMBA-treated animals by 24-56% relative to NMBA controls. This inhibition correlated with reductions in the formation of the NMBA-induced O6-methylguanine adduct in esophageal DNA, suggesting that the berries influenced the metabolism of NMBA leading to reduced DNA damage. Studies are ongoing to determine the mechanisms by which berries influence NMBA metabolism and DNA adduct formation. BRBs and STRWs were also tested in a postinitiation scheme and were found to inhibit NMBA-induced esophageal tumorigenesis by 31-64% when administered in the diet following treatment of the animals with NMBA. Berries, therefore, inhibit tumor promotion and progression events as well as tumor initiation. In vivo mechanistic studies with BRBs indicate that they reduce the growth rate of premalignant esophageal cells, in part, through down-regulation of cyclooxygenase-2 leading to reduced prostaglandin production and of inducible nitric oxide synthase leading to reduced nitrate/nitrite levels in the esophagus. Based upon the preclinical data on rodents, we have initiated prevention trials in humans to determine if berries might exhibit chemopreventive effects in the esophagus.
CitationNutr. Cancer 2006, 54 (1):33-46
JournalNutrition and Cancer
DescriptionDietary modulation of cancer & cancer biomarkers; Dietary modulation of carcinogenesis-related pathways. Dietary item or component studied: black raspberries (Rubus occidentalis, BRBs); blackberries (R. fructicosus, BBs); strawberries (Fragaria ananasia, STRWs)Outcome studied: esophageal tumors (papillomas); DNA damage. Study type: Fischer 344 rats. Tissue/biological material/sample size: esophagus. Mode of exposure: dietary. Impact on outcome (including dose-response): At 25 wk of the bioassay, all three berry types were found to inhibit the number of esophageal tumors (papillomas) in NMBA-treated animals by 24-56% relative to NMBA controls. This inhibition correlated with reductions in the formation of the NMBA-induced O6-methylguanine adduct in esophageal DNA. BRBs and STRWs were also tested in a postinitiation scheme and were found to inhibit NMBA-induced esophageal tumorigenesis by 31-64% when administered in the diet following treatment of the animals with NMBA. KEYWORDS - CLASSIFICATION: administration & dosage;analogs & derivatives;analysis;Animals;antagonists & inhibitors;Anticarcinogenic Agents;chemically induced;chemistry;Cyclooxygenase 2;Diet;dietary modulation of cancer & cancer biomarkers;dietary modulation of carcinogenesis-related pathways;Dimethylnitrosamine;Dinoprostone;DNA Adducts;drug effects;Esophageal Neoplasms;Esophagus;Food Preservation;Fragaria;Freeze Drying;Fruit;Gene Expression;genetics;Guanosine;humans;mechanisms of carcinogenesis;metabolism;Nitric Oxide Synthase Type II;Nitrites;Phytotherapy;prevention & control;Proliferating Cell Nuclear Antigen;Rats;Rats,Inbred F344;Research;Rosaceae;Weight Gain;
- Mechanistic basis for the chemopreventive effects of black raspberries at a late stage of rat esophageal carcinogenesis.
- Authors: Wang LS, Dombkowski AA, Seguin C, Rocha C, Cukovic D, Mukundan A, Henry C, Stoner GD
- Issue date: 2011 Apr
- Chemopreventive properties of black raspberries in N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis: down-regulation of cyclooxygenase-2, inducible nitric oxide synthase, and c-Jun.
- Authors: Chen T, Hwang H, Rose ME, Nines RG, Stoner GD
- Issue date: 2006 Mar 1
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- Authors: Kresty LA, Morse MA, Morgan C, Carlton PS, Lu J, Gupta A, Blackwood M, Stoner GD
- Issue date: 2001 Aug 15
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