Clove (Syzygium aromaticum L.), a potential chemopreventive agent for lung cancer.
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AffiliationDepartment of Cancer Chemoprevention, Chittarajan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700026, India.
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AbstractSpices and flavoring plants part rich in supposedly health-promoting phytochemicals are currently receiving much attention as a possible source of cancer chemopreventive compounds. Clove, the sun-dried unopened flower bud from the plant Syzygium aromaticum L. is a commonly used spice and food flavor. In the present work we assess the chemopreventive potential of aqueous infusion of clove during benzo[a]pyrene (BP)-induced lung carcinogenesis in strain A mice. Incidence of hyperplasia, dysplasia and carcinoma in situ evident in the carcinogen control group on the 8th, 17th and 26th weeks, respectively, were effectively reduced after treatment with clove infusion. Significant reduction in the number of proliferating cells and an increased number of apoptotic cells was also noted in these BP-induced lung lesions following clove treatment. Western blotting analysis revealed that clove infusion upregulates the expression of pro-apoptotic proteins p53 and Bax, and downregulates the expression of anti-apoptotic protein Bcl-2 in the precancerous stages. Expression of caspase 3 and its activation by clove infusion were evident from a very early stage of carcinogenesis (eighth week). Clove infusion was also found to downregulate the expression of some growth-promoting proteins, viz, COX-2, cMyc, Hras. The observations signify the chemopreventive potential of clove in view of its apoptogenic and anti-proliferative properties.
CitationCarcinogenesis 2006, 27 (8):1645-1654
Description. Dietary modulation of cancer & cancer biomarkers Dietary item or component studied:clove (syzygium aromaticum L)Outcome studied (cancer or cancer biomarker): lung cancerStudy type (in vitro, animals, humans): strain A miceTissue/biological material/sample size: 4 μm serial lung sectionsMode of exposure (if in vivo):100μl/mouse/day at the sub-scapular region (single dose)Impact on outcome (including dose-response):hyperplasia was reduced by 12.5%, P<0.01Dysplasia was reduced by 28.57%, P<0.01Carcinoma in situ was reduced by 85.71%, P<0.001Dietary modulation of carcinogenesis-related pathwaysDietary item or component studied:clove (syzygium aromaticum L)Pathways studied:apoptosisStudy type (in vitro, animals, humans): strain A miceTissue/biological material/sample size:4 μm serial lung sectionsMode of exposure (if in vivo) (acute, chronic, root of exposure):100μl/mouse/day at the sub-scapular region (single dose)Impact on pathway (including dose-response):proliferation: 64.29% reduction,P<0.001)Inhibition of Apoptosis: 1.56+/-0.25 in the exposed group, 1.72+/-0.21 after the treatmentP53: 14.12% increase on the 17th week and 46.53% on the 26th week. BAX: 16.11% on the 17th week and 53.88% on the 26th weekBcl-2: 47.29 % and 56.15% reduction on the 17th and 26th week respectivelyCOX: a 13.49 and 55.93% reduction on the 17th and 26th week respectivelycMyc: a 15.16% reduction on the 26th week Hras: a 45.52% reduction on the 26th week. KEYWORDS CLASSIFICATION: analysis;Animals;Animals,Newborn;Apoptosis;bcl-2-Associated X Protein;Benzo(a)pyrene;chemistry;Carcinoma in Situ;Cell Proliferation;Chemoprevention;Cyclooxygenase 2;dietary modulation of cancer & cancer biomarkers;dietary modulation of carcinogenesis-related pathways;drug effects;drug therapy;Eugenia;Female;Genes,ras;Hyperplasia;Incidence;India;Lung Neoplasms;metabolism;Mice;pharmacology;physiology;prevention & control;Plant Extracts;Proteins;Proto-Oncogene Proteins;Proto-Oncogene Proteins c-bcl-2;Research;Spices;toxicity;Tumor Suppressor Protein p53.