• Login
    View Item 
    •   Home
    • ECNIS annotated biomarkers bibliographic database - test version
    • Articles with annotation
    • View Item
    •   Home
    • ECNIS annotated biomarkers bibliographic database - test version
    • Articles with annotation
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ECNIS-NIOMCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsSubject (MeSH)PublisherJournalDepartmentThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsSubject (MeSH)PublisherJournalDepartment

    My Account

    LoginRegister

    External Links

    NIOMNIOMProjectsSherpa/RoMEO

    Statistics

    Display statistics

    Phenylethyl isothiocyanate and its N-acetylcysteine conjugate suppress the metastasis of SK-Hep1 human hepatoma cells.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Hwang, Eun-Sun
    Lee, Hyong Joo
    Issue Date
    2006-12
    
    Metadata
    Show full item record
    Abstract
    Phenylethyl isothiocyanate (PEITC), a hydrolysis compound of gluconasturtiin, is metabolized to N-acetylcysteine (NAC)-PEITC in the body after the consumption of cruciferous vegetables. We observed an inhibitory effect of PEITC and its metabolite NAC-PEITC on cancer cell proliferation, adhesion, invasion, migration and metastasis in SK-Hep1 human hepatoma cells. PEITC and NAC-PEITC suppressed SK-Hep1 cell proliferation in a dose-dependent manner, and exposure to 10 microM PEITC or NAC-PEITC reduced cell proliferation by 25% and 30%, respectively. NAC-PEITC inhibited cancer cell adhesion, invasion and migration to a similar or to an even larger degree than PEITC. The expression of matrix metalloproteinase (MMP) 2, MMP-9 and membrane type 1 matrix metalloproteinase (MT1-MMP) is a known risk factor for metastatic disease. Gelatin zymography analysis revealed a significant downregulation of MMP-2/MMP-9 protein expression in SK-Hep1 cells treated with 0.1-5 microM PEITC or NAC-PEITC. PEITC and NAC-PEITC treatment caused dose-dependent decreases in MMP-2/MMP-9 and MT1-MMP mRNA levels, as determined by reverse transcription polymerase chain reaction. PEITC and NAC-PEITC also increased the mRNA levels of tissue inhibitors of matrix metalloproteinase (TIMPs) 1 and 2. Our data suggest that this inhibition is mediated by downregulation of MMP and upregulation of TIMPs.
    Citation
    J. Nutr. Biochem. 2006, 17 (12):837-846
    Journal
    The Journal of nutritional biochemistry
    URI
    http://hdl.handle.net/10146/54093
    DOI
    10.1016/j.jnutbio.2006.02.004
    PubMed ID
    16563723
    Additional Links
    http://www.jnutbio.com/article/S0955-2863(06)00048-9/abstract
    http://patient-research.elsevier.com/patientresearch/displayAbs?key=S0955286306000489&referrer=http%253A%252F%252Fwww.ncbi.nlm.nih.gov%252Fsites%252Fentrez
    http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T8P-4JJGB3G-7&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=800ab93dc78506319f4b77d4fbbffd59
    Type
    Article
    Language
    en
    Description
    Dietary modulation of carcinogenesis-related pathwaysDietary item or component studied:Phenylethyl isothiocyanate (PEITC), N-acetylcysteine (NAC)-PEITC Pathways studied:inhibition of MMP-2 and MMP-9 enzyme activities, influence metastasis (cell viability, adhesion, invasion) and migration.Study type (in vitro, animals, humans): SK-Hep1 human hepatocellular carcinoma cellsTissue/biological material/sample size:(5103/well) in 96-well plates, 24-well plates, six-well plateImpact on pathway (including dose-response):PEITC inhibited cell proliferation by 9.6-69%, cell numbers decreased by42% and 65% at 5 and 10 AM NAC-PEITC, cellular motility was controlled in a time-dependent manner, decreased MMP-9 expression by 21% and 30% with 1 and 5 AM PEITC, NACPEITC, treatment at 0.1-5 AM did not show any inhibitory effect on MMP-9 activity, The inhibition of MMP-2 mRNA expression was greater than that of MMP-9 mRNA, with 40% and 80% inhibition by 5 AM AITC and NAC-AITC, respectively. KEYWORDS CLASSIFICATION: analysis;Acetylcysteine;Agriculture;Antineoplastic Agents;Biotechnology;chemistry;Carcinoma,Hepatocellular;Cell Adhesion;Cell Movement;drug effects;drug therapy;dietary modulation of carcinogenesis-related pathways;Drug Screening Assays,Antitumor;Food;genetics;Humans;Isothiocyanates;Korea;Liver Neoplasms;metabolism;Matrix Metalloproteinase 14;Matrix Metalloproteinase 2;Matrix Metalloproteinase 9;Neoplasm Invasiveness;Neoplasm Metastasis;pathology;pharmacology;Research;Tissue Inhibitor of Metalloproteinase-1;Tumor Cells,Cultured.
    ISSN
    0955-2863
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.jnutbio.2006.02.004
    Scopus Count
    Collections
    Articles with annotation

    entitlement

    Related articles

    • Benzyl isothiocyanate inhibits metalloproteinase-2/-9 expression by suppressing the mitogen-activated protein kinase in SK-Hep1 human hepatoma cells.
    • Authors: Hwang ES, Lee HJ
    • Issue date: 2008 Jul
    • Allyl isothiocyanate and its N-acetylcysteine conjugate suppress metastasis via inhibition of invasion, migration, and matrix metalloproteinase-2/-9 activities in SK-Hep 1 human hepatoma cells.
    • Authors: Hwang ES, Lee HJ
    • Issue date: 2006 Apr
    • Effects of phenylethyl isothiocyanate and its metabolite on cell-cycle arrest and apoptosis in LNCaP human prostate cancer cells.
    • Authors: Hwang ES, Lee HJ
    • Issue date: 2010 May
    • Association of a high activity of matrix metalloproteinase-9 to low levels of tissue inhibitors of metalloproteinase-1 and -3 in human hepatitis B-viral hepatoma cells.
    • Authors: Kim JR, Kim CH
    • Issue date: 2004 Nov
    • Effects of laver extracts on adhesion, invasion, and migration in SK-Hep1 human hepatoma cancer cells.
    • Authors: Do Thi N, Hwang ES
    • Issue date: 2014

    DSpace software (copyright © 2002 - 2019)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.