Acute hypoxia and reoxygenation-induced DNA oxidation in human mononuclear blood cells.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Thomsen, Jonas Juhl
MetadataShow full item record
AbstractResearch indicates that exposure to hypoxia is associated with oxidative stress. In this investigation, healthy subjects were exposed to hypoxia by inhalation of 10% oxygen for 2h (corresponding to 5500m above sea level). The levels of strand breaks and oxidatively damaged purine bases, measured by the comet assay, and the expression of genes involved in DNA repair of oxidatively damaged DNA were investigated in mononuclear blood cells (MNBC) at baseline, after 2h of hypoxia, 2h of reoxygenation, and 1 day and 8 days after the exposure. The level of strand breaks and oxidized purine bases in MNBC increased following both the 2h of hypoxia and the 2h reoxygenation period, whereas this effect was not observed in unexposed subjects. The expressions of oxoguanine DNA glycosylase 1 (OGG1), nucleoside diphosphate linked moiety X-type motif 1 (NUDT1), nei endonuclease VIII-like 1 (NEIL1), and mutY homolog (MUTYH) were unaltered throughout the experiment in both groups of subjects, indicating that DNA repair genes are not up-regulated by the hypoxia and reoxygenation treatment. Taken together, this report shows that inhalation of 10% oxygen for 2h is associated with increased number of oxidized DNA lesions in MNBC, but acute hypoxia may not inflict upon the regulation of genes involved in repair of oxidized DNA.
CitationMutat. Res. 2007, 625 (1-2):125-133
SponsorsThis study was supported by the Danish Research Agency. The authors (L.R., L.M., S.L., G.F., and P.M.) of this paper are partners of ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943).
- Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity.
- Authors: Jensen A, Løhr M, Eriksen L, Grønbæk M, Dorry E, Loft S, Møller P
- Issue date: 2012 Jan 1
- Exposure to ultrafine particles from ambient air and oxidative stress-induced DNA damage.
- Authors: Bräuner EV, Forchhammer L, Møller P, Simonsen J, Glasius M, Wåhlin P, Raaschou-Nielsen O, Loft S
- Issue date: 2007 Aug
- Expression of human oxoguanine glycosylase 1 or formamidopyrimidine glycosylase in human embryonic kidney 293 cells exacerbates methylmercury toxicity in vitro.
- Authors: Ondovcik SL, Preston TJ, McCallum GP, Wells PG
- Issue date: 2013 Aug 15
- No effect of 600 grams fruit and vegetables per day on oxidative DNA damage and repair in healthy nonsmokers.
- Authors: Møller P, Vogel U, Pedersen A, Dragsted LO, Sandström B, Loft S
- Issue date: 2003 Oct
- DNA damage and repair activity after broccoli intake in young healthy smokers.
- Authors: Riso P, Martini D, Møller P, Loft S, Bonacina G, Moro M, Porrini M
- Issue date: 2010 Nov