Measurement and meaning of oxidatively modified DNA lesions in urine.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractBACKGROUND: Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety of diseases. The noninvasive assessment of such damage, i.e., in urine, and application to large-scale human studies are vital to understanding this role and devising intervention strategies. METHODS: We have reviewed the literature to establish the status quo with regard to the methods and meaning of measuring DNA oxidation products in urine. RESULTS: Most of the literature focus upon 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), and whereas a large number of these reports concern clinical conditions, there remains (a) lack of consensus between methods, (b) possible contribution from diet and/or cell death, (c) no definitive DNA repair source of urinary 2'-deoxyribonucleoside lesions, and (d) no reference ranges for healthy or diseased individuals. CONCLUSIONS: The origin of 8-oxodG is not identified; however, recent cell culture studies suggest that the action of Nudix hydrolase(s) on oxidative modification of the nucleotide pool is a likely candidate for the 8-oxodG found in urine and, potentially, of other oxidized 2'-deoxyribonucleoside lesions. Literature reports suggest that diet and cell death have minimal, if any, influence upon urinary levels of 8-oxodG and 8-oxo-7,8-dihydroguanine, although this should be assessed on a lesion-by-lesion basis. Broadly speaking, there is consensus between chromatographic techniques; however, ELISA approaches continue to overestimate 8-oxodG levels and is not sufficiently specific for accurate quantification. With increasing numbers of lesions being studied, it is vital that these fundamental issues are addressed. We report the formation of the European Standards Committee on Urinary (DNA) Lesion Analysis whose primary goal is to achieve consensus between methods and establish reference ranges in health and disease.
CitationCancer Epidemiol. Biomarkers Prev. 2008, 17 (1):3-14
JournalCancer Epidemiology, Biomarkers & Prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
- DNA repair and the origins of urinary oxidized 2'-deoxyribonucleosides.
- Authors: Evans MD, Saparbaev M, Cooke MS
- Issue date: 2010 Sep
- DNA repair is responsible for the presence of oxidatively damaged DNA lesions in urine.
- Authors: Cooke MS, Evans MD, Dove R, Rozalski R, Gackowski D, Siomek A, Lunec J, Olinski R
- Issue date: 2005 Jul 1
- Biomarkers of oxidative damage to DNA and repair.
- Authors: Loft S, Høgh Danielsen P, Mikkelsen L, Risom L, Forchhammer L, Møller P
- Issue date: 2008 Oct
- Evidence for attenuated cellular 8-oxo-7,8-dihydro-2'-deoxyguanosine removal in cancer patients.
- Authors: Cooke MS, Rozalski R, Dove R, Gackowski D, Siomek A, Evans MD, Olinski R
- Issue date: 2006 Apr
- Interpretation of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine is adversely affected by methodological inaccuracies when using a commercial ELISA.
- Authors: Garratt LW, Mistry V, Singh R, Sandhu JK, Sheil B, Cooke MS, Sly PD, ARESTCF.
- Issue date: 2010 Jun 1