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dc.contributor.authorDziaman, Tomasz
dc.contributor.authorBanaszkiewicz, Zbigniew
dc.contributor.authorRoszkowski, Krzysztof
dc.contributor.authorGackowski, Daniel
dc.contributor.authorWisniewska, Ewa
dc.contributor.authorRozalski, Rafał
dc.contributor.authorFoksinski, Marek
dc.contributor.authorSiomek, Agnieszka
dc.contributor.authorSpeina, Elzbieta
dc.contributor.authorWinczura, Alicja
dc.contributor.authorMarszalek, Andrzej
dc.contributor.authorTudek, Barbara
dc.contributor.authorOlinski, Ryszard
dc.date.accessioned2013-09-04T08:07:14Z
dc.date.available2013-09-04T08:07:14Z
dc.date.issued2013-07-05
dc.identifier.citationInt. J. Cancer 2013:en_GB
dc.identifier.issn1097-0215
dc.identifier.pmid23832862
dc.identifier.doi10.1002/ijc.28374
dc.identifier.urihttp://hdl.handle.net/10146/300741
dc.description.abstractThe aim of this work was to answer the question whether the broad range of parameters which describe oxidative stress and oxidatively damaged DNA and repair are appropriate prognosis factors of colon cancer (CRC) patients survival? The following parameters were analyzed for 89 CRC patients: concentration of uric acid and vitamins A, E, C in plasma; levels of 8-oxodGuo (8-oxo-7,8-dihydro-2'-deoxyguanosine) in DNA of leukocyte and colon tissues; urinary excretion rates of 8-oxodGuo and 8-oxoGua (8-oxo-7,8-dihydroguanine); the activity and mRNA or protein level of repair enzymes OGG1, APE1, ANPG, TDG and PARP1. All DNA modifications and plasma antioxidants were analyzed using high performance liquid chromatography (HPLC) or HPLC/gas chromatography-mass spectrometry techniques. Expression of repair proteins was analyzed by QPCR, Western or immunohistochemistry methods. Longer survival coincided with low levels of 8-oxodGuo/8oxoGua in urine and 8-oxodGuo in DNA as well as with high concentration of uric acid plasma level. In contrast to expectations, longer survival coincided with lower mRNA level in normal colon tissue of the main 8-oxoGua DNA glycosylase, OGG1, but no association was found for PARP-1 expression. When analyzing simultaneously two parameters the discriminating power increased significantly. Combination of low level of urinary 8-oxoGua together with low level of 8-oxodGuo in leukocyte (both below median value) or high concentration of plasma uric acid (above median value) have the best prediction power. Since prediction value of these parameters seems to be comparable to conventional staging procedure, they could possibly be used as markers to predict clinical success in CRC treatment.
dc.description.sponsorshipThe authors of this article (R.O., T.D., R.R., D.G., A.S., M.F.) are partners of ECNIS and ECNIS2 (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a Network of Excellence operating within the European Union 6th and 7th Framework Program (contract numbers: 513943 and 266198, respectively).en_GB
dc.languageENG
dc.language.isoenen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1002/ijc.28374/abstract;jsessionid=009F3E8C2F08F7917F993B304636FFE8.d02t04en_GB
dc.rightsArchived with thanks to International journal of cancer. Journal international du canceren_GB
dc.subjectColon canceren_GB
dc.subjectSurvivalen_GB
dc.subjectDNA damageen_GB
dc.subjectOxidative stressen_GB
dc.subjectDNA repairen_GB
dc.subjectUric aciden_GB
dc.subject8-oxoGuaen_GB
dc.subjectBiological markersen_GB
dc.title8-Oxo-7,8-dihydroguanine and uric acid as efficient predictors of survival in colon cancer patients.en
dc.typeArticleen
dc.identifier.journalInternational Journal of Cancer. Journal international du canceren_GB
html.description.abstractThe aim of this work was to answer the question whether the broad range of parameters which describe oxidative stress and oxidatively damaged DNA and repair are appropriate prognosis factors of colon cancer (CRC) patients survival? The following parameters were analyzed for 89 CRC patients: concentration of uric acid and vitamins A, E, C in plasma; levels of 8-oxodGuo (8-oxo-7,8-dihydro-2'-deoxyguanosine) in DNA of leukocyte and colon tissues; urinary excretion rates of 8-oxodGuo and 8-oxoGua (8-oxo-7,8-dihydroguanine); the activity and mRNA or protein level of repair enzymes OGG1, APE1, ANPG, TDG and PARP1. All DNA modifications and plasma antioxidants were analyzed using high performance liquid chromatography (HPLC) or HPLC/gas chromatography-mass spectrometry techniques. Expression of repair proteins was analyzed by QPCR, Western or immunohistochemistry methods. Longer survival coincided with low levels of 8-oxodGuo/8oxoGua in urine and 8-oxodGuo in DNA as well as with high concentration of uric acid plasma level. In contrast to expectations, longer survival coincided with lower mRNA level in normal colon tissue of the main 8-oxoGua DNA glycosylase, OGG1, but no association was found for PARP-1 expression. When analyzing simultaneously two parameters the discriminating power increased significantly. Combination of low level of urinary 8-oxoGua together with low level of 8-oxodGuo in leukocyte (both below median value) or high concentration of plasma uric acid (above median value) have the best prediction power. Since prediction value of these parameters seems to be comparable to conventional staging procedure, they could possibly be used as markers to predict clinical success in CRC treatment.


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