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dc.contributor.authorLoft, Steffen
dc.contributor.authorDanielsen, Pernille
dc.contributor.authorLøhr, Mille
dc.contributor.authorJantzen, Kim
dc.contributor.authorHemmingsen, Jette G.
dc.contributor.authorRoursgaard, Martin
dc.contributor.authorKarotki, Dorina Gabriela
dc.contributor.authorMøller, Peter
dc.date.accessioned2013-07-22T06:56:28Z
dc.date.available2013-07-22T06:56:28Z
dc.date.issued2012-02-15
dc.identifier.citationArch. Biochem. Biophys. 2012, 518 (2):142-150en_GB
dc.identifier.issn1096-0384
dc.identifier.pmid22239988
dc.identifier.doi10.1016/j.abb.2011.12.026
dc.identifier.urihttp://hdl.handle.net/10146/296742
dc.description.abstractOxidatively damaged DNA may be important in carcinogenesis. 8-Oxo-7,8-dihydroguanine (8-oxoGua) is an abundant and mutagenic lesion excised by oxoguanine DNA glycosylase 1 (OGG1) and measurable in urine or plasma by chromatographic methods with electrochemical or mass spectrometric detectors, reflecting the rate of damage in steady state. A common genetic OGG1 variant may affect the activity and was associated with increased levels of oxidized purines in leukocytes without apparent effect on 8-oxoGua excretion or major change in cancer risk. 8-OxoGua excretion has been associated with exposure to air pollution, toxic metals, tobacco smoke and low plasma antioxidant levels, whereas fruit and vegetable intake or dietary interventions showed no association. In rodent studies some types of feed may be source of 8-oxoGua in collected urine. Of cancer therapies, cisplatin increased 8-oxoGua excretion, whereas radiotherapy only showed such effects in experimental animals. Case-control studies found high excretion of 8-oxoGua in relation to cancer, dementia and celiac disease but not hemochromatosis, although associations could be a consequence rather than reflecting causality of disease. One prospective study found increased risk of developing lung cancer among non-smokers associated with high excretion of 8-oxoGua. Urinary excretion of 8-oxoGua is a promising biomarker of oxidatively damaged DNA.
dc.description.sponsorshipThe authors are supported by the Danish Research Councils, Center for Indoor Air and Health in Dwellings (CISBO supported by Realdania), the Ingeborg and Leo Dannin Foundation and ECNIS2, a coordination and support action within the European FP7.en_GB
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0003986111004516en_GB
dc.rightsArchived with thanks to Archives of biochemistry and biophysicsen_GB
dc.subjectCancer risken_GB
dc.subjectBiomarkersen_GB
dc.subjectDNA damageen_GB
dc.subjectReactive oxygen speciesen_GB
dc.subjectDNA Glycosylasesen_GB
dc.subjectUrinary excretionen_GB
dc.subjectAnimalsen_GB
dc.subjectHumansen_GB
dc.subjectDNA repairen_GB
dc.subject.meshAnimals
dc.subject.meshBiological Markers
dc.subject.meshCeliac Disease
dc.subject.meshDNA Damage
dc.subject.meshDNA Glycosylases
dc.subject.meshDementia
dc.subject.meshHumans
dc.subject.meshNeoplasms
dc.subject.meshOxidation-Reduction
dc.subject.meshRisk Factors
dc.subject.meshTumor Markers, Biological
dc.titleUrinary excretion of 8-oxo-7,8-dihydroguanine as biomarker of oxidative damage to DNA.en
dc.typeArticleen
dc.identifier.journalArchives of Biochemistry and Biophysicsen_GB
html.description.abstractOxidatively damaged DNA may be important in carcinogenesis. 8-Oxo-7,8-dihydroguanine (8-oxoGua) is an abundant and mutagenic lesion excised by oxoguanine DNA glycosylase 1 (OGG1) and measurable in urine or plasma by chromatographic methods with electrochemical or mass spectrometric detectors, reflecting the rate of damage in steady state. A common genetic OGG1 variant may affect the activity and was associated with increased levels of oxidized purines in leukocytes without apparent effect on 8-oxoGua excretion or major change in cancer risk. 8-OxoGua excretion has been associated with exposure to air pollution, toxic metals, tobacco smoke and low plasma antioxidant levels, whereas fruit and vegetable intake or dietary interventions showed no association. In rodent studies some types of feed may be source of 8-oxoGua in collected urine. Of cancer therapies, cisplatin increased 8-oxoGua excretion, whereas radiotherapy only showed such effects in experimental animals. Case-control studies found high excretion of 8-oxoGua in relation to cancer, dementia and celiac disease but not hemochromatosis, although associations could be a consequence rather than reflecting causality of disease. One prospective study found increased risk of developing lung cancer among non-smokers associated with high excretion of 8-oxoGua. Urinary excretion of 8-oxoGua is a promising biomarker of oxidatively damaged DNA.


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