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dc.contributor.authorJohansson-Persson, Anna
dc.contributor.authorBarri, Thaer
dc.contributor.authorUlmius, Matilda
dc.contributor.authorOnning, Gunilla
dc.contributor.authorDragsted, Lars Ove
dc.date.accessioned2013-05-07T07:30:58Z
dc.date.available2013-05-07T07:30:58Z
dc.date.issued2013-05
dc.identifier.citationAnal Bioanal Chem 2013, 405 (14):4799-4809en_GB
dc.identifier.issn1618-2650
dc.identifier.pmid23535740
dc.identifier.doi10.1007/s00216-013-6874-5
dc.identifier.urihttp://hdl.handle.net/10146/288554
dc.description.abstractThe objective was to investigate the alterations of plasma metabolome profiles to identify exposure and effect markers of dietary fiber intake. Subjects (n = 25) aged 58.6 (1.1) years (mean and SD) with a body mass index of 26.6 (0.5) kg/m(2) were given a high fiber (HF) and a low fiber (LF) diet, in a 5-week randomized controlled crossover intervention. The HF diet consisted of oat bran, rye bran, and sugar beet fiber incorporated into test food products, whereas the LF diet was made of equivalent food products to the HF diet, but without adding fibers. Blood plasma samples were collected at the start and end of each intervention period and analyzed by LC-QTOF/MS. In total, 6 features in positive mode and 14 features in negative mode were significantly different between the HF and the LF diet (p < 0.01, q < 0.05). Two markers, 2,6-dihydroxybenzoic acid and 2-aminophenol sulfate, were increased after HF diet, along with a tentatively identified saponin derived from oat avenacosides. The untargeted metabolomics approach enabled the identification of two new markers of dietary fiber intake in human plasma. Further studies will be needed to verify if these markers could serve as compliance markers of fiber intake.
dc.description.sponsorshipThis work has been supported by Nordic Centre of Excellence SYSDIET (Systems biology in controlled dietary interventions and cohort studies, no. 070014), OPUS (Optimal wellbeing, development and health for Danish children through a healthy New Nordic Diet, supported by a grant from the Nordea Foundation), the European Network of Excellence NuGO (The European Nutrigenomics Organization), the EU Network of Excellence ECNIS2 (Environmental Cancer Risk , Nutrition and Individual Susceptibility) and a VINNOVA grant (project number 2004–02285). Biomedical Nutrition, Lund University and the Department of Nutrition, Exercise and Sports, University of Copenhagen are members of the NuGO Association.en_GB
dc.language.isoenen
dc.relation.urlhttp://link.springer.com/article/10.1007%2Fs00216-013-6874-5en_GB
dc.rightsArchived with thanks to Analytical and bioanalytical chemistryen_GB
dc.subjectDietary fiberen_GB
dc.subjectOaten_GB
dc.subjectRyeen_GB
dc.subjectSugar beeten_GB
dc.subjectHumanen_GB
dc.subjectFooden_GB
dc.subjectMetabolomicsen_GB
dc.subjectBlood plasmaen_GB
dc.subjectBiomarkersen_GB
dc.subject2,6-dihydroxybenzoic aciden_GB
dc.subject2-aminophenol sulfateen_GB
dc.subjectLC-QTOF/MSen_GB
dc.titleLC-QTOF/MS metabolomic profiles in human plasma after a 5-week high dietary fiber intake.en
dc.typeArticleen
dc.identifier.journalAnalytical and Bioanalytical Chemistryen_GB
html.description.abstractThe objective was to investigate the alterations of plasma metabolome profiles to identify exposure and effect markers of dietary fiber intake. Subjects (n = 25) aged 58.6 (1.1) years (mean and SD) with a body mass index of 26.6 (0.5) kg/m(2) were given a high fiber (HF) and a low fiber (LF) diet, in a 5-week randomized controlled crossover intervention. The HF diet consisted of oat bran, rye bran, and sugar beet fiber incorporated into test food products, whereas the LF diet was made of equivalent food products to the HF diet, but without adding fibers. Blood plasma samples were collected at the start and end of each intervention period and analyzed by LC-QTOF/MS. In total, 6 features in positive mode and 14 features in negative mode were significantly different between the HF and the LF diet (p < 0.01, q < 0.05). Two markers, 2,6-dihydroxybenzoic acid and 2-aminophenol sulfate, were increased after HF diet, along with a tentatively identified saponin derived from oat avenacosides. The untargeted metabolomics approach enabled the identification of two new markers of dietary fiber intake in human plasma. Further studies will be needed to verify if these markers could serve as compliance markers of fiber intake.


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