Urinary measurement of 8-OxodG, 8-OxoGua, and 5HMUra: a noninvasive assessment of oxidative damage to DNA.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Cooke, Marcus S.
MetadataShow full item record
AbstractNumerous DNA repair pathways exist to prevent the persistence of damage, and are integral to the maintenance of genome stability, and hence prevention of disease. Excised lesions arising from repair may ultimately appear in the urine where their measurement has been acknowledged to be reflective of overall oxidative stress. The development of reliable assays to measure urinary DNA lesions, such as HPLC prepurification followed by gas chromatography/mass spectrometry, offers the potential to assess whole body oxidative DNA damage. However, some studies suggest a possibility that confounding factors may contribute to urinary levels of 7,8-dihydro-8-oxoguanine (8-oxoGua) and 7,8-dihydro-8-oxo-2 -deoxyguanosine (8-oxodG). This article considers several possible sources of urinary lesions: (a) the repair of oxidatively damaged DNA; (b) a possible dietary influence; and (c) cell death. The authors conclude that data from their laboratories, along with a number of literature reports, form an argument against a contribution from cell death and diet. In the absence of these confounding factors, urinary measurements may be attributed entirely to the repair of DNA damage and suggests their possible use in studying associations between DNA repair and disease.
CitationAntioxid. Redox Signal. 2006, 8 (5-6):1011-1019
JournalAntioxidants & Redox Signaling
- DNA repair is responsible for the presence of oxidatively damaged DNA lesions in urine.
- Authors: Cooke MS, Evans MD, Dove R, Rozalski R, Gackowski D, Siomek A, Lunec J, Olinski R
- Issue date: 2005 Jul 1
- Small field radiotherapy of head and neck cancer patients is responsible for oxidatively damaged DNA/oxidative stress on the level of a whole organism.
- Authors: Roszkowski K, Gackowski D, Rozalski R, Dziaman T, Siomek A, Guz J, Szpila A, Foksinski M, Olinski R
- Issue date: 2008 Oct 15
- Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer.
- Authors: Roszkowski K, Jozwicki W, Blaszczyk P, Mucha-Malecka A, Siomek A
- Issue date: 2011 Jun
- Diet is not responsible for the presence of several oxidatively damaged DNA lesions in mouse urine.
- Authors: Rozalski R, Siomek A, Gackowski D, Foksinski M, Gran C, Klungland A, Olinski R
- Issue date: 2004 Nov
- Effects of basal level of antioxidants on oxidative DNA damage in humans.
- Authors: Foksinski M, Gackowski D, Rozalski R, Siomek A, Guz J, Szpila A, Dziaman T, Olinski R
- Issue date: 2007 Apr