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dc.contributor.authorSingh, Rajinder
dc.contributor.authorGromadzinska, Jolanta
dc.contributor.authorMistry, Yogita
dc.contributor.authorCordell, Rebecca
dc.contributor.authorJuren, Tina
dc.contributor.authorSegerback, Dan
dc.contributor.authorFarmer, Peter B.
dc.date.accessioned2012-11-26T12:32:36Z
dc.date.available2012-11-26T12:32:36Z
dc.date.issued2012-09-01
dc.identifier.citationMutat. Res. 2012, 737 (1-2):8-11en_GB
dc.identifier.issn0027-5107
dc.identifier.pmid22824164
dc.identifier.doi10.1016/j.mrfmmm.2012.07.001
dc.identifier.urihttp://hdl.handle.net/10146/253333
dc.description.abstractEpidemiological studies have shown an association between alcohol (ethanol) consumption and increased cancer risk. The effect of alcohol consumption on the levels and persistence of N(2)-ethylidene-2'-deoxyguanosine (N(2)-ethylidene-dG) formed by acetaldehyde, the oxidative metabolite of ethanol, in human leukocyte DNA was investigated. DNA was isolated from venous blood samples obtained from 30 male non-smoking individuals before consumption of alcohol (0h) and subsequently at 3-5h following the consumption of 150mL of vodka (containing 42% pure ethanol). Additional samples were collected 24h and 48h post-alcohol consumption. The levels of N(2)-ethyl-2'-deoxyguanosine (N(2)-ethyl-dG) in the DNA were determined following reduction of N(2)-ethylidene-dG with sodium cyanoborohydride using a liquid chromatography-tandem mass spectrometry selected reaction monitoring method. A slight time-dependent trend showing an increase and decrease in the levels of N(2)-ethyl-dG was observed following consumption of alcohol compared to time 0h, however, the differences were not statistically significant. The average levels of N(2)-ethyl-dG observed at 0h, 3-5h, 24h and 48h time points following ingestion of alcohol were 34.6±21.9, 35.1±21.0, 36.8±20.7 and 35.6±21.1 per 10(8) 2'-deoxynucleosides, respectively. In conclusion, alcohol consumption that could be encountered under social drinking conditions, does not significantly alter the levels of the acetaldehyde derived DNA adduct, N(2)-ethyl-dG in human leukocyte DNA from healthy individuals.
dc.description.sponsorshipThe European Union Network of Excellence, Environmental Cancer, Nutrition and Individual Susceptibility (ECNIS, Contract No. FOOD-CT-2005- 513943).en_GB
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0027510712001698#en_GB
dc.rightsArchived with thanks to Mutation researchen_GB
dc.subjectAlcohol consumptionen_GB
dc.subjectAcetaldehydeen_GB
dc.subjectDNA adductsen_GB
dc.subjectN2-ethyl-2′-deoxyguanosineen_GB
dc.subjectHumanen_GB
dc.subjectLeukocytesen_GB
dc.subjectLiquid chromatographyen_GB
dc.subjectMass spectrometryen_GB
dc.subject.meshAcetaldehydeen
dc.subject.meshAdulten
dc.subject.meshAlcohol Drinkingen
dc.subject.meshChromatography, Liquiden
dc.subject.meshDNAen
dc.subject.meshDNA Adductsen
dc.subject.meshDeoxyguanosineen
dc.subject.meshHumansen
dc.subject.meshLeukocytesen
dc.subject.meshMaleen
dc.subject.meshSpectrometry, Mass, Electrospray Ionizationen
dc.subject.meshTandem Mass Spectrometryen
dc.subject.meshTime Factorsen
dc.subject.meshYoung Adulten
dc.titleDetection of acetaldehyde derived N(2)-ethyl-2'-deoxyguanosine in human leukocyte DNA following alcohol consumption.en
dc.typeArticleen
dc.identifier.journalMutation Researchen_GB
html.description.abstractEpidemiological studies have shown an association between alcohol (ethanol) consumption and increased cancer risk. The effect of alcohol consumption on the levels and persistence of N(2)-ethylidene-2'-deoxyguanosine (N(2)-ethylidene-dG) formed by acetaldehyde, the oxidative metabolite of ethanol, in human leukocyte DNA was investigated. DNA was isolated from venous blood samples obtained from 30 male non-smoking individuals before consumption of alcohol (0h) and subsequently at 3-5h following the consumption of 150mL of vodka (containing 42% pure ethanol). Additional samples were collected 24h and 48h post-alcohol consumption. The levels of N(2)-ethyl-2'-deoxyguanosine (N(2)-ethyl-dG) in the DNA were determined following reduction of N(2)-ethylidene-dG with sodium cyanoborohydride using a liquid chromatography-tandem mass spectrometry selected reaction monitoring method. A slight time-dependent trend showing an increase and decrease in the levels of N(2)-ethyl-dG was observed following consumption of alcohol compared to time 0h, however, the differences were not statistically significant. The average levels of N(2)-ethyl-dG observed at 0h, 3-5h, 24h and 48h time points following ingestion of alcohol were 34.6±21.9, 35.1±21.0, 36.8±20.7 and 35.6±21.1 per 10(8) 2'-deoxynucleosides, respectively. In conclusion, alcohol consumption that could be encountered under social drinking conditions, does not significantly alter the levels of the acetaldehyde derived DNA adduct, N(2)-ethyl-dG in human leukocyte DNA from healthy individuals.


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