Detection of acetaldehyde derived N(2)-ethyl-2'-deoxyguanosine in human leukocyte DNA following alcohol consumption.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Farmer, Peter B.
MetadataShow full item record
AbstractEpidemiological studies have shown an association between alcohol (ethanol) consumption and increased cancer risk. The effect of alcohol consumption on the levels and persistence of N(2)-ethylidene-2'-deoxyguanosine (N(2)-ethylidene-dG) formed by acetaldehyde, the oxidative metabolite of ethanol, in human leukocyte DNA was investigated. DNA was isolated from venous blood samples obtained from 30 male non-smoking individuals before consumption of alcohol (0h) and subsequently at 3-5h following the consumption of 150mL of vodka (containing 42% pure ethanol). Additional samples were collected 24h and 48h post-alcohol consumption. The levels of N(2)-ethyl-2'-deoxyguanosine (N(2)-ethyl-dG) in the DNA were determined following reduction of N(2)-ethylidene-dG with sodium cyanoborohydride using a liquid chromatography-tandem mass spectrometry selected reaction monitoring method. A slight time-dependent trend showing an increase and decrease in the levels of N(2)-ethyl-dG was observed following consumption of alcohol compared to time 0h, however, the differences were not statistically significant. The average levels of N(2)-ethyl-dG observed at 0h, 3-5h, 24h and 48h time points following ingestion of alcohol were 34.6±21.9, 35.1±21.0, 36.8±20.7 and 35.6±21.1 per 10(8) 2'-deoxynucleosides, respectively. In conclusion, alcohol consumption that could be encountered under social drinking conditions, does not significantly alter the levels of the acetaldehyde derived DNA adduct, N(2)-ethyl-dG in human leukocyte DNA from healthy individuals.
CitationMutat. Res. 2012, 737 (1-2):8-11
SponsorsThe European Union Network of Excellence, Environmental Cancer, Nutrition and Individual Susceptibility (ECNIS, Contract No. FOOD-CT-2005- 513943).
- Evaluation of the DNA damaging potential of cannabis cigarette smoke by the determination of acetaldehyde derived N2-ethyl-2'-deoxyguanosine adducts.
- Authors: Singh R, Sandhu J, Kaur B, Juren T, Steward WP, Segerbäck D, Farmer PB
- Issue date: 2009 Jun
- Sequence distribution of acetaldehyde-derived N2-ethyl-dG adducts along duplex DNA.
- Authors: Matter B, Guza R, Zhao J, Li ZZ, Jones R, Tretyakova N
- Issue date: 2007 Oct
- Kinetics of DNA adduct formation in the oral cavity after drinking alcohol.
- Authors: Balbo S, Meng L, Bliss RL, Jensen JA, Hatsukami DK, Hecht SS
- Issue date: 2012 Apr
- Stability of acetaldehyde-derived DNA adduct in vitro.
- Authors: Hori K, Miyamoto S, Yukawa Y, Muto M, Chiba T, Matsuda T
- Issue date: 2012 Jul 13
- N2-ethyldeoxyguanosine as a potential biomarker for assessing effects of alcohol consumption on DNA.
- Authors: Balbo S, Hashibe M, Gundy S, Brennan P, Canova C, Simonato L, Merletti F, Richiardi L, Agudo A, Castellsagué X, Znaor A, Talamini R, Bencko V, Holcátová I, Wang M, Hecht SS, Boffetta P
- Issue date: 2008 Nov