Evidence for attenuated cellular 8-oxo-7,8-dihydro-2'-deoxyguanosine removal in cancer patients.
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AuthorsCooke, Marcus S.
Evans, Mark D.
MetadataShow full item record
AbstractMeasurement of the products of oxidatively damaged DNA in urine is a frequently used means by which oxidative stress may be assessed non-invasively. We believe that urinary DNA lesions, in addition to being biomarkers of oxidative stress, can potentially provide more specific information, for example, a reflection of repair activity. We used high-performance liquid chromatography prepurification, with gas chromatography-mass spectrometry (LC-GC-MS) and ELISA to the analysis of a number of oxidative [e.g., 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydro-guanine, 5-(hydroxymethyl)uracil], non-oxidative (cyclobutane thymine dimers) and oligomeric DNA products in urine. We analysed spot urine samples from 20 healthy subjects, and 20 age- and sex-matched cancer patients. Mononuclear cell DNA 8-oxodG levels were assessed by LC-EC. The data support our proposal that urinary DNA lesion products are predominantly derived from DNA repair. Furthermore, analysis of DNA and urinary 8-oxodG in cancer patients and controls suggested reduced repair activity towards this lesion marker in these patients.
CitationBiol. Chem. 2006, 387 (4):393-400
SponsorsMSC and MDE would like to acknowledge financial support from the UK Food Standards Agency, and Arthritis Research Campaign. RO acknowledges partial financial support by a grant from the Committee for Science Research (No. PBZ-KBN-091/ PO5/55). The authors also acknowledge financial support from the EU NoE ‘ECNIS’ grant FOOD-CT-2005-513943. The authors acknowledge the assistance of the Department of Chemical Pathology, Leicester Royal Infirmary, UK, for performing the analysis of urinary uric acid.
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