Oxidative stress and DNA damage caused by the urban air pollutant 3-NBA and its isomer 2-NBA in human lung cells analyzed with three independent methods.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe air pollutant 3-nitrobenzanthrone (3-NBA), emitted in diesel exhaust, is a potent mutagen and genotoxin. 3-NBA can isomerise to 2-nitrobenzanthrone (2-NBA), which can become more than 70-fold higher in concentration in ambient air. In this study, three independent methods have been employed to evaluate the oxidative stress and genotoxicity of 2-NBA compared to 3-NBA in the human A549 lung cell line. HPLC-EC/UV was applied for measurements of oxidative damage in the form of 8-oxo-2'-deoxyguanosine (8-oxodG), (32)P-HPLC for measurements of lipophilic DNA-adducts, and the Comet assay to measure a variety of DNA lesions, including oxidative stress. No significant oxidative damage from either isomer was found regarding formation of 8-oxodG analysed using HPLC-EC/UV. However, the Comet assay (with FPG-treatment), which is more sensitive and detects more types of damages compared to HPLC-EC/UV, showed a significant effect from both 3-NBA and 2-NBA. (32)P-HPLC revealed a strong DNA-adduct formation from both 3-NBA and 2-NBA, and also a significant difference between both isomers compared to negative control. These results clearly show that 2-NBA has a genotoxic potential. Even if the DNA-adduct forming capacity and the amount of DNA lesions measured with the (32)P-HPLC and Comet assay is about one third of 3-NBA, the high abundance of 2-NBA in ambient air calls for further investigation and evaluation of its health hazard.
CitationJ. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2005, 827(1):94-103
SponsorsThis project was supported by the Swedish Environmental Protection Agency. The authors of this paper are partners of the EU network of ECNIS (European Cancer Risk, Nutrition and Individual Susceptibility).
- DNA adduct formation and oxidative stress from the carcinogenic urban air pollutant 3-nitrobenzanthrone and its isomer 2-nitrobenzanthrone, in vitro and in vivo.
- Authors: Nagy E, Adachi S, Takamura-Enya T, Zeisig M, Möller L
- Issue date: 2007 Mar
- Mutagenicity and DNA adduct formation by the urban air pollutant 2-nitrobenzanthrone.
- Authors: Arlt VM, Glatt H, Gamboa da Costa G, Reynisson J, Takamura-Enya T, Phillips DH
- Issue date: 2007 Aug
- Activation and detoxification metabolism of urban air pollutants 2-nitrobenzanthrone and carcinogenic 3-nitrobenzanthrone by rat and mouse hepatic microsomes.
- Authors: Stiborova M, Cechova T, Borek-Dohalska L, Moserova M, Frei E, Schmeiser HH, Paca J, Arlt VM
- Issue date: 2012
- Mechanisms of the different DNA adduct forming potentials of the urban air pollutants 2-nitrobenzanthrone and carcinogenic 3-nitrobenzanthrone.
- Authors: Stiborová M, Martínek V, Svobodová M, Sístková J, Dvorák Z, Ulrichová J, Simánek V, Frei E, Schmeiser HH, Phillips DH, Arlt VM
- Issue date: 2010 Jul 19
- Identification of three major DNA adducts formed by the carcinogenic air pollutant 3-nitrobenzanthrone in rat lung at the C8 and N2 position of guanine and at the N6 position of adenine.
- Authors: Arlt VM, Schmeiser HH, Osborne MR, Kawanishi M, Kanno T, Yagi T, Phillips DH, Takamura-Enya T
- Issue date: 2006 May 1