Stability and reproducibility of simultaneously detected plasma and serum cytokine levels in asymptomatic subjects.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsSaberi Hosnijeh, Fatemeh
Krop, Esmeralda J. M.
Rabkin, Charles S.
MetadataShow full item record
AbstractBlood levels of cyto- and chemokines might reflect immune deregulations which might be related to lymphomagenesis. Potential utility of stored blood samples of a prospective cohort was evaluated by the effect of different blood sample types and freeze-thaw cycles on analyte levels. Bead-based immunoassays were performed on two fresh samples (serum, citrate and heparin plasma) of 10 asymptomatic adults collected 14 days apart and on aliquots of the first samples which were put through one to three freeze-thaw cycles to measure 11 cytokines, four chemokines and two adhesion molecules. Median coefficients of variation (CVs) of the measured analytes were 20%, 24% and 32% in serum, citrate and heparin plasma, respectively. Strong correlations (rank correlation coefficient 0.74-0.98) were observed between sample types, although small differences in analyte levels were observed for most analytes. Freeze-thaw cycles did not markedly change analyte levels. Our study supports the use of this assay among asymptomatic subjects in epidemiological studies.
CitationBiomarkers 2010, 15 (2):140-148
SponsorsThis work was supported by the ECNIS Network of Excellence (Environmental Cancer Risk, Nutrition and Individual Susceptibility), operating within the European Union 6th Framework Program, Priority 5: ‘Food Quality and Safety (FOOD-CT-2005- 513943). The first author also acknowledges the Iranian Ministry of Health and Medical Education for support of a PhD programme at Utrecht University.
- Influence of pre-analytical and analytical factors on soluble CD40L measurements.
- Authors: Varo N, Nuzzo R, Natal C, Libby P, Schönbeck U
- Issue date: 2006 Nov
- Immunoassay and antibody microarray analysis of the HUPO Plasma Proteome Project reference specimens: systematic variation between sample types and calibration of mass spectrometry data.
- Authors: Haab BB, Geierstanger BH, Michailidis G, Vitzthum F, Forrester S, Okon R, Saviranta P, Brinker A, Sorette M, Perlee L, Suresh S, Drwal G, Adkins JN, Omenn GS
- Issue date: 2005 Aug
- Influence of blood sampling procedure on plasma concentrations of matrix metalloproteinases and their tissue inhibitors.
- Authors: Rossignol P, Cambillau M, Bissery A, Mouradian D, Benetos A, Michel JB, Plouin PF, Chatellier G, Jacob MP
- Issue date: 2008 Apr
- Clinical evaluation of the levels of 12 cytokines in serum/plasma under various storage conditions using evidence biochip arrays.
- Authors: Guo GH, Dong J, Yuan XH, Dong ZN, Tian YP
- Issue date: 2013 Mar
- Effects of sample handling on the stability of interleukin 6, tumour necrosis factor-alpha and leptin.
- Authors: Flower L, Ahuja RH, Humphries SE, Mohamed-Ali V
- Issue date: 2000 Nov