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dc.contributor.authorMoller, Peter
dc.contributor.authorCooke, Marcus S.
dc.contributor.authorCollins, Andrew
dc.contributor.authorOlinski, Ryszard
dc.contributor.authorRozalski, Rafal
dc.contributor.authorLoft, Steffen
dc.date.accessioned2012-07-04T10:01:17Z
dc.date.available2012-07-04T10:01:17Z
dc.date.issued2012-04
dc.identifier.citationFree Radic. Res. 2012, 46 (4):541-553en_GB
dc.identifier.issn1029-2470
dc.identifier.pmid22117555
dc.identifier.doi10.3109/10715762.2011.644241
dc.identifier.urihttp://hdl.handle.net/10146/232054
dc.description.abstractLevels of oxidatively damaged cellular DNA and urinary excretion of damaged 2'-deoxyribonuclosides are widely measured in biomonitoring studies examining the role of oxidative stress induced by environmental exposures, lifestyle factors and development of disease. This has promoted efforts to harmonise measurements of oxidised guanine nucleobases by the variety of analytical approaches for DNA and urinary levels of damage, in multi-laboratory trials that are centred in Europe. The large inter-laboratory variation reported of values of oxidatively damaged DNA is reduced by harmonising assay protocols. Recent attention on optimal conditions for the comet assay may lead to better understanding of the most critical steps in procedure, which generate variation in DNA damage levels between laboratories. Measurements of urinary excretion of oxidatively generated 8-oxo-7,8-dihydro-2'-deoxyguanosine also show large differences between different methods, where chromatographic techniques generally show more reliable results than antibody-based methods. In this case, standardising calibrants is aimed at improving within technique agreement.
dc.description.sponsorshipThis work was 752 supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th 753 Framework Program, Priority 5: 754 “Food Quality and Safety” (contract 513943) and “ECNIS: towards ECNIS Centre for Research and 755 Education on Cancer, Environment and Food” (grant agreement no: 266198)”. Peter Møller and 756 Steffen Loft are supported by grants from the Centre for Indoor air and Health in Dwellings 757 (Realdania Research) and The Centre for Pharmaceutical Nanotechnology and Nanotoxicology 758 (Danish Council for Strategic Research).en_GB
dc.language.isoenen
dc.relation.urlhttp://informahealthcare.com/doi/abs/10.3109/10715762.2011.644241en_GB
dc.rightsArchived with thanks to Free radical researchen_GB
dc.subjectBiological Markersen_GB
dc.subjectDeoxyadenosinesen_GB
dc.subjectDNA damageen_GB
dc.subjectDNAen_GB
dc.subjectUrineen_GB
dc.subjectAnimalsen_GB
dc.subjectHumansen_GB
dc.subjectEnzyme-Linked Immunosorbent Assayen_GB
dc.subject.meshAnimals
dc.subject.meshBiological Markers
dc.subject.meshDNA
dc.subject.meshDNA Damage
dc.subject.meshDeoxyadenosines
dc.subject.meshEnzyme-Linked Immunosorbent Assay
dc.subject.meshHumans
dc.subject.meshOxidation-Reduction
dc.titleHarmonising measurements of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA and urine.en
dc.typeArticleen
dc.identifier.journalFree Radical Researchen_GB
html.description.abstractLevels of oxidatively damaged cellular DNA and urinary excretion of damaged 2'-deoxyribonuclosides are widely measured in biomonitoring studies examining the role of oxidative stress induced by environmental exposures, lifestyle factors and development of disease. This has promoted efforts to harmonise measurements of oxidised guanine nucleobases by the variety of analytical approaches for DNA and urinary levels of damage, in multi-laboratory trials that are centred in Europe. The large inter-laboratory variation reported of values of oxidatively damaged DNA is reduced by harmonising assay protocols. Recent attention on optimal conditions for the comet assay may lead to better understanding of the most critical steps in procedure, which generate variation in DNA damage levels between laboratories. Measurements of urinary excretion of oxidatively generated 8-oxo-7,8-dihydro-2'-deoxyguanosine also show large differences between different methods, where chromatographic techniques generally show more reliable results than antibody-based methods. In this case, standardising calibrants is aimed at improving within technique agreement.


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