Regulation of selenoprotein mRNA expression by hormones and retinoic acid in bovine mammary cells.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractSelenium is essential for maintaining many body functions through the actions of selenoproteins. To find factors regulating selenoprotein biosynthesis in the bovine mammary cell line MAC-T, the effects of supplementation with selenite and also with retinoic acid, insulin, hydrocortisone and prolactin on the mRNA expression of a number of selenoproteins were investigated. It was found that MAC-T cells express glutathione peroxidase (GPx) 1 and 4, thioredoxin reductase 1 and selenoprotein P, but not GPx 3, which is interesting considering that GPx 3 is one of the only few selenoproteins detected in milk so far. Addition of selenite to the cell culture resulted in a large increase in GPx 1 expression and an increase in selenoprotein P expression, which is similar to the findings made in other systems investigated. Increased mRNA levels of GPx 1 were also observed in cells treated with insulin and hydrocortisone or with retinoic acid. The expression of thioredoxin reductase 1 was increased in cells treated with retinoic acid, whereas that of selenoprotein P was decreased in cells exposed to insulin. The results indicate that several hormones, selenium, and retinoic acid regulate the biosynthesis of various selenoproteins differently in the bovine mammary cell. The possible implications of the findings for processes related to milk formation and mammary carcinogenesis will need additional investigation. Further study of the detailed mechanisms involved is also necessary.
CitationJ. Trace Elem. Med. Biol. 2010, 24 (4):251-256
SponsorsThese studies were supported by the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas), the Lund University Hospital, and the Påhlsson Foundation. Biomedical Nutrition is a member of the EU NoE, The European Nutrigenomics Organisation (NuGO, no. 505944) and the NoE, Environmental Carcinogenesis, Nutrition and Individual Susceptibility (ECNIS, no. 513943), which partly supported the study.
- Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue.
- Authors: Bruzelius K, Hoac T, Sundler R, Onning G, Akesson B
- Issue date: 2007 Feb
- The influence of selenium-enriched milk proteins and selenium yeast on plasma selenium levels and rectal selenoprotein gene expression in human subjects.
- Authors: Hu Y, McIntosh GH, Le Leu RK, Upton JM, Woodman RJ, Young GP
- Issue date: 2011 Aug
- Biosynthesis of selenoproteins in cultured bovine mammary cells.
- Authors: Bruzelius K, Purup S, James P, Onning G, Akesson B
- Issue date: 2008
- Growth hormone and lactogenic hormones can reduce the leptin mRNA expression in bovine mammary epithelial cells.
- Authors: Yonekura S, Sakamoto K, Komatsu T, Hagino A, Katoh K, Obara Y
- Issue date: 2006 Jul
- Selenoproteins in bladder cancer.
- Authors: Reszka E
- Issue date: 2012 May 18