Effect of sonication and serum proteins on copper release from copper nanoparticles and the toxicity towards lung epithelial cells.
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Karlsson, Hanna L.
Wallinder, Inger Odnevall
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AbstractAbstract Different methodological settings can influence particle characteristics and toxicity in nanotoxicology. The aim of this study was to investigate how serum proteins and sonication of Cu nanoparticle suspensions influence the properties of the nanoparticles and toxicological responses on human lung epithelial cells. This was investigated by using methods for particle characterization (photon correlation spectroscopy and TEM) and Cu release (atomic absorption spectroscopy) in combination with assays for analyzing cell toxicity (MTT-, trypan blue- and Comet assay). The results showed that sonication of Cu nanoparticles caused decreased cell viability and increased Cu release compared to non-sonicated particles. Furthermore, serum in the cell medium resulted in less particle agglomeration and increased Cu release compared with medium without serum, but no clear difference in toxicity was detected. Few cells showed intracellular Cu nanoparticles due to fast release/dissolution processes of Cu. In conclusion; sonication can affect the toxicity of nanoparticles.
CitationNanotoxicology 2011, 5 (2):269-281
SponsorsThis work was partly supported by the Swedish Research Council for Environment, Agricultural Sciences, and Spatial Planning (FORMAS), AForsk (Angpanneföreningen’s Foundation for Research and Development), and Environmental Cancer Risk, Nutrition, and Individual Susceptibility (ECNIS), a Network of Excellence operating within the European Union 6th Framework Program, Priority 5: “Food, Quality and Safety” (Contract No. 513943).
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