Reference genes for gene expression studies on non-small cell lung cancer.
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AbstractSTUDY OBJECTIVE: The aim of this study was to test a panel of 6 reference genes in order to identify and validate the most suitable reference genes for expression studies in paired healthy and non-small cell lung cancer tissues. METHOD: Quantitative real-time PCR followed by the NormFinder- and geNorm-based analysis was employed. The study involved 21 non-small cell lung cancer patients. RESULTS: The analysis of experimental data revealed HPRT1 as the most stable gene followed by RPLP0 and ESD. In contrast, GAPDH was found to be the least stable gene. HPRT1 together with ESD was revealed as the pair of genes introducing the least systematic error into data normalization. Validation by bootstrap random sampling technique and by normalizing exemplary gene expression data confirmed the results. CONCLUSION: Although HPRT1 and ESD may by recommended for data normalization in gene expression studies on non-small cell lung cancer, the suitability of selected reference genes must be unconditionally validated prior to each study.
CitationActa Biochim. Pol. 2009, 56 (2):307-316
JournalActa Biochimica Polonica
SponsorsThis work was partially supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No. 513943). Dr. Peter Gresner is a fellow of EPITOK (Transfer of Knowledge in Molecular Biology and Epidemiology of Occupational and Environmental Cancer), a Marie Curie Action within the European Union 6th Framework Program (No. MTKDCT- 2004–509829).
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