Expression of selenoprotein-coding genes SEPP1, SEP15 and hGPX1 in non-small cell lung cancer.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractAim of the study was to investigate the mRNA expression level of selenoprotein P (SEPP1), 15-kDa selenoprotein (SEP15) and glutathione peroxidase 1 (hGPX1) in paired malignant and non-malignant tissue. To achieve this goal, the quantitative real-time PCR technique was utilized in paired tissue samples from 33 non-small cell lung cancer (NSCLC) patients. Simultaneously, the activity of glutathione peroxidases (GPX) and the level of thiobarbituric acid-reactive species (TBARS) in paired tissue specimens and the blood plasma selenium level was measured. We found significant down-regulation of SEPP1 expression level in tumorous lung tissue (2.732-fold; p<0.001). The expression of hGPX1 and SEP15 in tumorous tissue remained unchanged compared to healthy tissue. The level of TBARS in malignant tissue was significantly increased (p<0.005) and negatively correlated with SEPP1 expression level (R(S)=-0.3238; p<0.05). The activity of GPX in malignant tissue was significantly increased compared to the non-malignant one (p<0.005) and negatively correlated with the expression level of SEPP1. It seems possible, that the down-regulation of SEPP1 expression may lead to an increased oxidative stress possibly resulting in lung carcinogenesis. Increased activity of GPX in tumorous lung tissue seems to be a feedback mechanism.
CitationLung Cancer 2009, 65 (1):34-40
SponsorsThis work was partially supported by ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No 513943). Dr Peter Gresner is a fellow of EPITOK (Transfer of Knowledge in Molecular Biology and Epidemiology of Occupational and Environmental Cancer), a Marie Curie Action within the European Union 6th Framework Program (No MTKDCT-2004-509829).
- The influence of selenium-enriched milk proteins and selenium yeast on plasma selenium levels and rectal selenoprotein gene expression in human subjects.
- Authors: Hu Y, McIntosh GH, Le Leu RK, Upton JM, Woodman RJ, Young GP
- Issue date: 2011 Aug
- Down-regulation of Gadd45 expression is associated with tumor differentiation in non-small cell lung cancer.
- Authors: Higashi H, Vallböhmer D, Warnecke-Eberz U, Hokita S, Xi H, Brabender J, Metzger R, Baldus SE, Natsugoe S, Aikou T, Hölscher AH, Schneider PM
- Issue date: 2006 May-Jun
- Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue.
- Authors: Bruzelius K, Hoac T, Sundler R, Onning G, Akesson B
- Issue date: 2007 Feb
- Variation in the selenoenzyme genes and risk of advanced distal colorectal adenoma.
- Authors: Peters U, Chatterjee N, Hayes RB, Schoen RE, Wang Y, Chanock SJ, Foster CB
- Issue date: 2008 May
- Expression profiling and genetic alterations of the selenoproteins GI-GPx and SePP in colorectal carcinogenesis.
- Authors: Al-Taie OH, Uceyler N, Eubner U, Jakob F, Mörk H, Scheurlen M, Brigelius-Flohe R, Schöttker K, Abel J, Thalheimer A, Katzenberger T, Illert B, Melcher R, Köhrle J
- Issue date: 2004