Circadian gene methylation in rotating-shift nurses: a cross-sectional study.

2.50
Hdl Handle:
http://hdl.handle.net/10146/618216
Title:
Circadian gene methylation in rotating-shift nurses: a cross-sectional study.
Authors:
Reszka, Edyta; Wieczorek, Edyta; Przybek, Monika; Jabłońska, Ewa; Kałużny, Paweł; Bukowska-Damska, Agnieszka; Zienolddiny, Shanbeh; Pepłońska, Beata
Abstract:
Investigating the methylation status of the circadian genes may contribute to a better understanding of the shift work-related circadian disruption in individuals exposed to artificial light at night. In the present study, we determined the methylation status of the circadian genes associated with a shift work pattern among nurses and midwives participating in a cross-sectional study in Lodz, Poland. Quantitative methylation polymerase chain reaction assays were used to assess promoter CpG methylation in PER1, PER2, PER3, CRY1, CRY2, BMAL1, CLOCK, and NPAS2 in genomic DNA from whole blood of 347 women having a rotating-shift work schedule and 363 women working days only. The percentage of methylated reference (PMR) was assessed using fluorescent probes for PER1, PER2, PER3, CRY1, and NPAS2, and the percentage of gene methylation, as the methylation index (MI), using two sets of primers for BMAL1, CLOCK, and CRY2. We tested the possible association between current and lifetime rotating night-shift work characteristics and circadian gene methylation by using proportional odds regression model with blood DNA methylation, categorized into tertiles, and adjusted for age, current smoking status, folate intake and blood collection time. The findings indicated that CpG methylation in PER2 promoter was significantly decreased (P < 0.004) among nurses and midwives currently working rotating shifts, as compared with day-working nurses and midwives. The lower percentage of PER2 methylation was associated with a higher monthly frequency of current night duties (2-7 night shifts, and eight or more night shifts per month) (P = 0.012) and was associated at borderline significance (P = 0.092) with the lifetime duration of shift work (>10 ≤ 20 years and >20 ≤ 43 years of rotating-shift work) among nurses and midwives (N = 710). Moreover, women with a longer lifetime duration of shift work presented a lower status of PER1 methylation (P = 0.040) than did the women with up to 10 years of rotating-shift work. Long lifetime duration of shift work (> 10 years) among current rotating night-shift workers (N =  347) was associated with BMAL1 hypomethylation (P = 0.013). Among eight of the investigated circadian genes, only PER1, PER2, and BMAL1 showed differential methylation attributable to the rotating-shift work of nurses and midwives. The findings on blood-based DNA methylation in the circadian genes may provide a better insight into the mechanistic principles underlying the possible health effects of night-shift work but these should be verified in further studies recruiting larger populations of shift workers.
Affiliation:
Nofer Institute of Occupational Medicine
Citation:
Chronobiol. Int. 2018, 35 (1):111-121
Journal:
Chronobiology International
Issue Date:
2018
URI:
http://hdl.handle.net/10146/618216
DOI:
10.1080/07420528.2017.1388252
PubMed ID:
29144171
Additional Links:
https://www.tandfonline.com/doi/abs/10.1080/07420528.2017.1388252?journalCode=icbi20
Type:
Article
Language:
en
ISSN:
1525-6073
Sponsors:
This work was supported by the Nofer Institute of Occupational Medicine [IMP10.32] and the Polish-Norwegian Research Program carried out by the National Centre for Research and Development [Pol-Nor/196940/22/2013 - CLOCKSHIFT].
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorReszka, Edytaen
dc.contributor.authorWieczorek, Edytaen
dc.contributor.authorPrzybek, Monikaen
dc.contributor.authorJabłońska, Ewaen
dc.contributor.authorKałużny, Pawełen
dc.contributor.authorBukowska-Damska, Agnieszkaen
dc.contributor.authorZienolddiny, Shanbehen
dc.contributor.authorPepłońska, Beataen
dc.date.accessioned2018-12-03T09:46:45Z-
dc.date.available2018-12-03T09:46:45Z-
dc.date.issued2018-
dc.identifier.citationChronobiol. Int. 2018, 35 (1):111-121en
dc.identifier.issn1525-6073-
dc.identifier.pmid29144171-
dc.identifier.doi10.1080/07420528.2017.1388252-
dc.identifier.urihttp://hdl.handle.net/10146/618216-
dc.description.abstractInvestigating the methylation status of the circadian genes may contribute to a better understanding of the shift work-related circadian disruption in individuals exposed to artificial light at night. In the present study, we determined the methylation status of the circadian genes associated with a shift work pattern among nurses and midwives participating in a cross-sectional study in Lodz, Poland. Quantitative methylation polymerase chain reaction assays were used to assess promoter CpG methylation in PER1, PER2, PER3, CRY1, CRY2, BMAL1, CLOCK, and NPAS2 in genomic DNA from whole blood of 347 women having a rotating-shift work schedule and 363 women working days only. The percentage of methylated reference (PMR) was assessed using fluorescent probes for PER1, PER2, PER3, CRY1, and NPAS2, and the percentage of gene methylation, as the methylation index (MI), using two sets of primers for BMAL1, CLOCK, and CRY2. We tested the possible association between current and lifetime rotating night-shift work characteristics and circadian gene methylation by using proportional odds regression model with blood DNA methylation, categorized into tertiles, and adjusted for age, current smoking status, folate intake and blood collection time. The findings indicated that CpG methylation in PER2 promoter was significantly decreased (P < 0.004) among nurses and midwives currently working rotating shifts, as compared with day-working nurses and midwives. The lower percentage of PER2 methylation was associated with a higher monthly frequency of current night duties (2-7 night shifts, and eight or more night shifts per month) (P = 0.012) and was associated at borderline significance (P = 0.092) with the lifetime duration of shift work (>10 ≤ 20 years and >20 ≤ 43 years of rotating-shift work) among nurses and midwives (N = 710). Moreover, women with a longer lifetime duration of shift work presented a lower status of PER1 methylation (P = 0.040) than did the women with up to 10 years of rotating-shift work. Long lifetime duration of shift work (> 10 years) among current rotating night-shift workers (N =  347) was associated with BMAL1 hypomethylation (P = 0.013). Among eight of the investigated circadian genes, only PER1, PER2, and BMAL1 showed differential methylation attributable to the rotating-shift work of nurses and midwives. The findings on blood-based DNA methylation in the circadian genes may provide a better insight into the mechanistic principles underlying the possible health effects of night-shift work but these should be verified in further studies recruiting larger populations of shift workers.en
dc.description.sponsorshipThis work was supported by the Nofer Institute of Occupational Medicine [IMP10.32] and the Polish-Norwegian Research Program carried out by the National Centre for Research and Development [Pol-Nor/196940/22/2013 - CLOCKSHIFT].en
dc.language.isoenen
dc.relation.urlhttps://www.tandfonline.com/doi/abs/10.1080/07420528.2017.1388252?journalCode=icbi20en
dc.rightsArchived with thanks to Chronobiology internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectcircadian genesen
dc.subjectDNA methylationen
dc.subjectblooden
dc.subjectnursesen
dc.subjectRotating shiften
dc.titleCircadian gene methylation in rotating-shift nurses: a cross-sectional study.en
dc.typeArticleen
dc.contributor.departmentNofer Institute of Occupational Medicineen
dc.identifier.journalChronobiology Internationalen

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