Biomarkers of selenium status and antioxidant effect in workers occupationally exposed to mercury.

2.50
Hdl Handle:
http://hdl.handle.net/10146/618215
Title:
Biomarkers of selenium status and antioxidant effect in workers occupationally exposed to mercury.
Authors:
Kuras, Renata; Reszka, Edyta; Wieczorek, Edyta; Jablonska, Ewa; Gromadzinska, Jolanta; Malachowska, Beata; Kozlowska, Lucyna; Stanislawska, Magdalena; Janasik, Beata; Wasowicz, Wojciech
Abstract:
The present observation based research was designed to evaluate the influence of occupational human exposure to metallic mercury (Hg°) vapor on the biomarkers of selenium status involved in the antioxidant defense system. For this purpose we determined Hg and selenium (Se) concentrations in body fluids, the markers of antioxidant effect measured as an activity of Se-dependent enzymes (red blood cell and plasma glutathione peroxidase: GPx1-RBC and GPx3-P), concentration of selenoprotein P in the plasma (SeP-P) and total antioxidant activity in the plasma (TAA-P) in 131 male workers from a chloralkali plant exposed to Hg° and 67 non-exposed males (control group). The mRNA expression levels of glutathione peroxidases (GPX1, GPX3), selenoprotein P (SEPP1), thioredoxin reductase 1 (TRXR1), thioredoxin 1 (TRX1), peroxiredoxins (PRDX1, PRDX2) were also examined in the leukocytes of peripheral blood. Hg concentration in the blood (Hg-B) and urine (Hg-U) samples was determined using the thermal decomposition amalgamation/atomic absorption spectrometry (TDA-AAS) method and Se concentrations in plasma (Se-P) and urine (Se-U) using the inductively coupled plasma mass spectrometry (ICP-MS) method. Activities of GPx1-RBC, GPx3-P and TAA-P were determined using the kinetic and spectrophotometric method, respectively. Gene expression analysis was performed using the quantitative Real-Time PCR. The results showed significant higher Hg levels among the Hg°-exposed workers in comparison to control group (12-times higher median for Hg-B and almost 74-times higher median for Hg-U concentration in chloralkali workers). Se-P was also significantly higher (Me (median): 82.85 μg/L (IQR (interquartile range) 72.03-90.28 μg/L) for chloralkali workers vs. Me: 72.74 μg/L (IQR 66.25-80.14 μg/L) for control group; p = 0.0001) but interestingly correlated inversely with Hg-U in chloralkali workers suggesting depletion of the Se protection among the workers with the highest Hg-U concentration. The mRNA level for GPX1, PRXD1 were markedly but significantly higher in the workers compared to the control group. Moreover, concentrations of Hg-B and Hg-U among the workers were significantly positively correlated with the levels of selenoprotein P at both the mRNA and selenoprotein levels. In the multivariate model, after adjusting to cofounders (dental amalgam fillings, age, BMI, job seniority time, smoking), we confirmed that Hg-U concentration was inversely correlated with genes expression of TRXR1. This is the first comprehensive assessment of the impact of occupational exposure of workers to Hg° at both the mRNA and selenoprotein levels, with investigation of fish intake obtained by means of a questionnaire. These findings suggest that exposure to Hg° alters gene expression of the antioxidant enzymes and the level of Se-containing selenoproteins.
Affiliation:
Nofer Institute of Occupational Medicine
Citation:
J Trace Elem Med Biol 2018, 49:43-50
Journal:
Journal of Trace Elements in Medicine and Biology : organ of the Society for Minerals and Trace Elements (GMS)
Issue Date:
Sep-2018
URI:
http://hdl.handle.net/10146/618215
DOI:
10.1016/j.jtemb.2018.04.032
PubMed ID:
29895371
Additional Links:
https://www.sciencedirect.com/science/article/pii/S0946672X17309860?via%3Dihub
Type:
Article
Language:
en
ISSN:
1878-3252
Sponsors:
This work was supported by Nofer Institute of Occupational Medicine (internal grant no. 1.31/2014) and the Ministry of Science and Higher Education in Poland (grant no. 2013/11/B/NZ7/04934).
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DC FieldValue Language
dc.contributor.authorKuras, Renataen
dc.contributor.authorReszka, Edytaen
dc.contributor.authorWieczorek, Edytaen
dc.contributor.authorJablonska, Ewaen
dc.contributor.authorGromadzinska, Jolantaen
dc.contributor.authorMalachowska, Beataen
dc.contributor.authorKozlowska, Lucynaen
dc.contributor.authorStanislawska, Magdalenaen
dc.contributor.authorJanasik, Beataen
dc.contributor.authorWasowicz, Wojciechen
dc.date.accessioned2018-12-03T09:41:02Z-
dc.date.available2018-12-03T09:41:02Z-
dc.date.issued2018-09-
dc.identifier.citationJ Trace Elem Med Biol 2018, 49:43-50en
dc.identifier.issn1878-3252-
dc.identifier.pmid29895371-
dc.identifier.doi10.1016/j.jtemb.2018.04.032-
dc.identifier.urihttp://hdl.handle.net/10146/618215-
dc.description.abstractThe present observation based research was designed to evaluate the influence of occupational human exposure to metallic mercury (Hg°) vapor on the biomarkers of selenium status involved in the antioxidant defense system. For this purpose we determined Hg and selenium (Se) concentrations in body fluids, the markers of antioxidant effect measured as an activity of Se-dependent enzymes (red blood cell and plasma glutathione peroxidase: GPx1-RBC and GPx3-P), concentration of selenoprotein P in the plasma (SeP-P) and total antioxidant activity in the plasma (TAA-P) in 131 male workers from a chloralkali plant exposed to Hg° and 67 non-exposed males (control group). The mRNA expression levels of glutathione peroxidases (GPX1, GPX3), selenoprotein P (SEPP1), thioredoxin reductase 1 (TRXR1), thioredoxin 1 (TRX1), peroxiredoxins (PRDX1, PRDX2) were also examined in the leukocytes of peripheral blood. Hg concentration in the blood (Hg-B) and urine (Hg-U) samples was determined using the thermal decomposition amalgamation/atomic absorption spectrometry (TDA-AAS) method and Se concentrations in plasma (Se-P) and urine (Se-U) using the inductively coupled plasma mass spectrometry (ICP-MS) method. Activities of GPx1-RBC, GPx3-P and TAA-P were determined using the kinetic and spectrophotometric method, respectively. Gene expression analysis was performed using the quantitative Real-Time PCR. The results showed significant higher Hg levels among the Hg°-exposed workers in comparison to control group (12-times higher median for Hg-B and almost 74-times higher median for Hg-U concentration in chloralkali workers). Se-P was also significantly higher (Me (median): 82.85 μg/L (IQR (interquartile range) 72.03-90.28 μg/L) for chloralkali workers vs. Me: 72.74 μg/L (IQR 66.25-80.14 μg/L) for control group; p = 0.0001) but interestingly correlated inversely with Hg-U in chloralkali workers suggesting depletion of the Se protection among the workers with the highest Hg-U concentration. The mRNA level for GPX1, PRXD1 were markedly but significantly higher in the workers compared to the control group. Moreover, concentrations of Hg-B and Hg-U among the workers were significantly positively correlated with the levels of selenoprotein P at both the mRNA and selenoprotein levels. In the multivariate model, after adjusting to cofounders (dental amalgam fillings, age, BMI, job seniority time, smoking), we confirmed that Hg-U concentration was inversely correlated with genes expression of TRXR1. This is the first comprehensive assessment of the impact of occupational exposure of workers to Hg° at both the mRNA and selenoprotein levels, with investigation of fish intake obtained by means of a questionnaire. These findings suggest that exposure to Hg° alters gene expression of the antioxidant enzymes and the level of Se-containing selenoproteins.en
dc.description.sponsorshipThis work was supported by Nofer Institute of Occupational Medicine (internal grant no. 1.31/2014) and the Ministry of Science and Higher Education in Poland (grant no. 2013/11/B/NZ7/04934).en
dc.language.isoenen
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0946672X17309860?via%3Dihuben
dc.rightsArchived with thanks to Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectgene expressionen
dc.subjectMercuryen
dc.subjectoccupational exposureen
dc.subjectseleniumen
dc.subjectselenoproteinsen
dc.subject.meshAdult-
dc.subject.meshAntioxidants-
dc.subject.meshGlutathione Peroxidase-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMercury-
dc.subject.meshMiddle Aged-
dc.subject.meshPeroxiredoxins-
dc.subject.meshReal-Time Polymerase Chain Reaction-
dc.subject.meshSelenium-
dc.subject.meshSelenoprotein P-
dc.subject.meshSelenoproteins-
dc.subject.meshThioredoxin Reductase 1-
dc.subject.meshThioredoxins-
dc.titleBiomarkers of selenium status and antioxidant effect in workers occupationally exposed to mercury.en
dc.typeArticleen
dc.contributor.departmentNofer Institute of Occupational Medicineen
dc.identifier.journalJournal of Trace Elements in Medicine and Biology : organ of the Society for Minerals and Trace Elements (GMS)en
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