Altered circadian genes expression in breast cancer tissue according to the clinical characteristics

2.50
Hdl Handle:
http://hdl.handle.net/10146/618213
Title:
Altered circadian genes expression in breast cancer tissue according to the clinical characteristics
Authors:
Lesicka, Monika; Jabłońska, Ewa; Wieczorek, Edyta; Seroczyńska, Barbara; Siekierzycka, Anna; Skokowski, Jarosław; Kalinowski, Leszek; Wąsowicz, Wojciech; Reszka, Edyta
Abstract:
Breast cancer has a multifactorial etiology. One of the supposed and novel mechanisms is an alteration of circadian gene expression. Circadian genes play a crucial role in many physiological processes. These processes, such as genomic stability, DNA repair mechanism and apoptosis, are frequently disrupted in breast tumors. To assess the significance of circadian gene expression in breast cancer, we carried out an analysis of CLOCK, BMAL1, NPAS2, PER1, PER2, PER3 and CRY1, CRY2, TIMELESS, CSNK1E expression by the use of the quantitative Real-Time PCR technique in tumor tissue and non-tumor adjacent normal tissue sampled from 107 women with a newly diagnosed disease. The obtained data were compared to the clinical and histopathological features. PER1, PER2, PER3, CRY2 were found to be significantly down-expressed, while CLOCK, TIMELESS were over-expressed in the studied tumor samples compared to the non-tumor samples. Only gene expression of CRY1 was significantly down-regulated with progression according to the TNM classification. We found significantly decreased expression of CRY2, PER1, PER2 genes in the ER/PR negative breast tumors compared to the ER/PR positive tumors. Additionally, expression of CRY2, NPAS2 genes had a decreased level in the poorly differentiated tumors in comparison with the well and moderately differentiated ones. Our results indicate that circadian gene expression is altered in breast cancer tissue, which confirms previous observations from various animal and in vitro studies.
Affiliation:
Nofer Institute of Occupational Medicine
Citation:
PLoS ONE 2018, 13 (6):e0199622
Journal:
PloS one
Issue Date:
2018
URI:
http://hdl.handle.net/10146/618213
DOI:
10.1371/journal.pone.0199622
PubMed ID:
29958276
Additional Links:
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0199622
Type:
Article
Language:
en
ISSN:
1932-6203
Sponsors:
This work was supported by the National Science Center (Grant No. 2014/15/N/NZ5/01671) (to ML), https://www.ncn.gov.pl/?language=en, and partially supported by Ministry of Science and Higher Education Poland DIR/WK/2017/01 (to LK), http://www.nauka.gov.pl/en/, and Internal grant for statutory activity at Nofer Institute of Occupational Medicine IMP 14.4/2018. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Full metadata record

DC FieldValue Language
dc.contributor.authorLesicka, Monikaen
dc.contributor.authorJabłońska, Ewaen
dc.contributor.authorWieczorek, Edytaen
dc.contributor.authorSeroczyńska, Barbaraen
dc.contributor.authorSiekierzycka, Annaen
dc.contributor.authorSkokowski, Jarosławen
dc.contributor.authorKalinowski, Leszeken
dc.contributor.authorWąsowicz, Wojciechen
dc.contributor.authorReszka, Edytaen
dc.date.accessioned2018-12-03T09:23:49Z-
dc.date.available2018-12-03T09:23:49Z-
dc.date.issued2018-
dc.identifier.citationPLoS ONE 2018, 13 (6):e0199622en
dc.identifier.issn1932-6203-
dc.identifier.pmid29958276-
dc.identifier.doi10.1371/journal.pone.0199622-
dc.identifier.urihttp://hdl.handle.net/10146/618213-
dc.description.abstractBreast cancer has a multifactorial etiology. One of the supposed and novel mechanisms is an alteration of circadian gene expression. Circadian genes play a crucial role in many physiological processes. These processes, such as genomic stability, DNA repair mechanism and apoptosis, are frequently disrupted in breast tumors. To assess the significance of circadian gene expression in breast cancer, we carried out an analysis of CLOCK, BMAL1, NPAS2, PER1, PER2, PER3 and CRY1, CRY2, TIMELESS, CSNK1E expression by the use of the quantitative Real-Time PCR technique in tumor tissue and non-tumor adjacent normal tissue sampled from 107 women with a newly diagnosed disease. The obtained data were compared to the clinical and histopathological features. PER1, PER2, PER3, CRY2 were found to be significantly down-expressed, while CLOCK, TIMELESS were over-expressed in the studied tumor samples compared to the non-tumor samples. Only gene expression of CRY1 was significantly down-regulated with progression according to the TNM classification. We found significantly decreased expression of CRY2, PER1, PER2 genes in the ER/PR negative breast tumors compared to the ER/PR positive tumors. Additionally, expression of CRY2, NPAS2 genes had a decreased level in the poorly differentiated tumors in comparison with the well and moderately differentiated ones. Our results indicate that circadian gene expression is altered in breast cancer tissue, which confirms previous observations from various animal and in vitro studies.en
dc.description.sponsorshipThis work was supported by the National Science Center (Grant No. 2014/15/N/NZ5/01671) (to ML), https://www.ncn.gov.pl/?language=en, and partially supported by Ministry of Science and Higher Education Poland DIR/WK/2017/01 (to LK), http://www.nauka.gov.pl/en/, and Internal grant for statutory activity at Nofer Institute of Occupational Medicine IMP 14.4/2018. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.language.isoenen
dc.relation.urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0199622en
dc.rightsArchived with thanks to PloS oneen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectcircadianen
dc.subjectbreast canceren
dc.titleAltered circadian genes expression in breast cancer tissue according to the clinical characteristicsen
dc.typeArticleen
dc.contributor.departmentNofer Institute of Occupational Medicineen
dc.identifier.journalPloS oneen
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