Influence of dispersion medium on nanomaterial-induced pulmonary inflammation and DNA strand breaks: investigation of carbon black, carbon nanotubes and three titanium dioxide nanoparticles.

2.50
Hdl Handle:
http://hdl.handle.net/10146/618201
Title:
Influence of dispersion medium on nanomaterial-induced pulmonary inflammation and DNA strand breaks: investigation of carbon black, carbon nanotubes and three titanium dioxide nanoparticles.
Authors:
Hadrup, Niels; Bengtson, Stefan; Jacobsen, Nicklas R; Jackson, Petra; Nocun, Marek ( 0000-0002-3160-3977 ) ; Saber, Anne T; Jensen, Keld A; Wallin, Håkan; Vogel, Ulla
Abstract:
Intratracheal instillation serves as a model for inhalation exposure. However, for this, materials are dispersed in appropriate media that may influence toxicity. We tested whether different intratracheal instillation dispersion media influence the pulmonary toxicity of different nanomaterials. Rodents were intratracheally instilled with 162 µg/mouse/1620 µg/rat carbon black (CB), 67 µg/mouse titanium dioxide nanoparticles (TiO2) or 54 µg/mouse carbon nanotubes (CNT). The dispersion media were as follows: water (CB, TiO2); 2% serum in water (CB, CNT, TiO2); 0.05% serum albumin in water (CB, CNT, TiO2); 10% bronchoalveolar lavage fluid in 0.9% NaCl (CB), 10% bronchoalveolar lavage (BAL) fluid in water (CB) or 0.1% Tween-80 in water (CB). Inflammation was measured as pulmonary influx of neutrophils into bronchoalveolar fluid, and DNA damage as DNA strand breaks in BAL cells by comet assay. Inflammation was observed for all nanomaterials (except 38-nm TiO2) in all dispersion media. For CB, inflammation was dispersion medium dependent. Increased levels of DNA strand breaks for CB were observed only in water, 2% serum and 10% BAL fluid in 0.9% NaCl. No dispersion medium-dependent effects on genotoxicity were observed for TiO2, whereas CNT in 2% serum induced higher DNA strand break levels than in 0.05% serum albumin. In conclusion, the dispersion medium was a determinant of CB-induced inflammation and genotoxicity. Water seemed to be the best dispersion medium to mimic CB inhalation, exhibiting DNA strand breaks with only limited inflammation. The influence of dispersion media on nanomaterial toxicity should be considered in the planning of intratracheal investigations.
Affiliation:
Nofer Institute of Occupational Medicine, Lodz, Poland
Citation:
Mutagenesis 2017, 32 (6):581-597
Journal:
Mutagenesis
Issue Date:
31-Dec-2017
URI:
http://hdl.handle.net/10146/618201
DOI:
10.1093/mutage/gex042
PubMed ID:
29301028
Additional Links:
https://academic.oup.com/mutage/article/32/6/581/4782222
Type:
Article
Language:
en
ISSN:
1464-3804
Sponsors:
This work was supported by the Danish Centre for Nanosafety II; the European Union project: NANOGENOTOX (grant number n°2009 21 01) and the European Union Seventh Framework Programme (FP7/2007–2013) GLADIATOR (grant number FP7-604000).
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Full metadata record

DC FieldValue Language
dc.contributor.authorHadrup, Nielsen
dc.contributor.authorBengtson, Stefanen
dc.contributor.authorJacobsen, Nicklas Ren
dc.contributor.authorJackson, Petraen
dc.contributor.authorNocun, Mareken
dc.contributor.authorSaber, Anne Ten
dc.contributor.authorJensen, Keld Aen
dc.contributor.authorWallin, Håkanen
dc.contributor.authorVogel, Ullaen
dc.date.accessioned2018-02-21T11:01:18Z-
dc.date.available2018-02-21T11:01:18Z-
dc.date.issued2017-12-31-
dc.identifier.citationMutagenesis 2017, 32 (6):581-597en
dc.identifier.issn1464-3804-
dc.identifier.pmid29301028-
dc.identifier.doi10.1093/mutage/gex042-
dc.identifier.urihttp://hdl.handle.net/10146/618201-
dc.description.abstractIntratracheal instillation serves as a model for inhalation exposure. However, for this, materials are dispersed in appropriate media that may influence toxicity. We tested whether different intratracheal instillation dispersion media influence the pulmonary toxicity of different nanomaterials. Rodents were intratracheally instilled with 162 µg/mouse/1620 µg/rat carbon black (CB), 67 µg/mouse titanium dioxide nanoparticles (TiO2) or 54 µg/mouse carbon nanotubes (CNT). The dispersion media were as follows: water (CB, TiO2); 2% serum in water (CB, CNT, TiO2); 0.05% serum albumin in water (CB, CNT, TiO2); 10% bronchoalveolar lavage fluid in 0.9% NaCl (CB), 10% bronchoalveolar lavage (BAL) fluid in water (CB) or 0.1% Tween-80 in water (CB). Inflammation was measured as pulmonary influx of neutrophils into bronchoalveolar fluid, and DNA damage as DNA strand breaks in BAL cells by comet assay. Inflammation was observed for all nanomaterials (except 38-nm TiO2) in all dispersion media. For CB, inflammation was dispersion medium dependent. Increased levels of DNA strand breaks for CB were observed only in water, 2% serum and 10% BAL fluid in 0.9% NaCl. No dispersion medium-dependent effects on genotoxicity were observed for TiO2, whereas CNT in 2% serum induced higher DNA strand break levels than in 0.05% serum albumin. In conclusion, the dispersion medium was a determinant of CB-induced inflammation and genotoxicity. Water seemed to be the best dispersion medium to mimic CB inhalation, exhibiting DNA strand breaks with only limited inflammation. The influence of dispersion media on nanomaterial toxicity should be considered in the planning of intratracheal investigations.en
dc.description.sponsorshipThis work was supported by the Danish Centre for Nanosafety II; the European Union project: NANOGENOTOX (grant number n°2009 21 01) and the European Union Seventh Framework Programme (FP7/2007–2013) GLADIATOR (grant number FP7-604000).en
dc.language.isoenen
dc.relation.urlhttps://academic.oup.com/mutage/article/32/6/581/4782222en
dc.rightsArchived with thanks to Mutagenesisen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectcarbon nanotubesen
dc.subjectnanoparticlesen
dc.subjectDNA damageen
dc.subjectinflammationen
dc.titleInfluence of dispersion medium on nanomaterial-induced pulmonary inflammation and DNA strand breaks: investigation of carbon black, carbon nanotubes and three titanium dioxide nanoparticles.en
dc.typeArticleen
dc.contributor.departmentNofer Institute of Occupational Medicine, Lodz, Polanden
dc.identifier.journalMutagenesisen

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