Determination of 8-oxo-2'-deoxyguanosine and creatinine in murine and human urine by liquid chromatography/tandem mass spectrometry: application to chemoprevention studies.

2.50
Hdl Handle:
http://hdl.handle.net/10146/83713
Title:
Determination of 8-oxo-2'-deoxyguanosine and creatinine in murine and human urine by liquid chromatography/tandem mass spectrometry: application to chemoprevention studies.
Authors:
Teichert, Friederike; Verschoyle, Richard D.; Greaves, Peter; Thorpe, James F.; Mellon, J. Kilian; Steward, William P.; Farmer, Peter B.; Gescher, Andreas J.; Singh, Rajinder
Abstract:
Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) represents a non-invasive biomarker for oxidative stress and may be useful for monitoring chemotherapeutic and chemopreventive interventions associated with cancer-related alterations in oxidative stress. We describe the development and validation of two separate liquid chromatography/tandem mass spectrometry (LC/MS/MS) selected reaction monitoring (SRM) methods for the determination of 8-oxodG and creatinine in both murine and human urine using stable isotope labelled internal standards. Levels of 8-oxodG were normalised to creatinine. The LC/MS/MS methods were applied to two chemoprevention studies utilising tea polyphenols in humans and TRAMP (TRansgenic Adenocarcinoma of the Mouse Prostate) mice. Patients with benign prostatic hyperplasia received 1 g/day of green tea polyphenols (GTP), 1 g/day of black tea theaflavins (BTT) or no treatment for 4 weeks. TRAMP mice received GTP (0.05% in drinking water) for 4 or 25 weeks. Prostate pathology in TRAMP mice was not affected by GTP. Levels of 8-oxodG were not altered by tea polyphenols in either mice or humans. In TRAMP mice, urinary 8-oxodG levels were elevated with increasing age (p < 0.0001) but not changed by the presence of prostate tumours. In conclusion, the LC/MS/MS SRM methods described here are ideally suited for the accurate determination of 8-oxodG and creatinine in urine samples from both clinical and pre-clinical studies.
Citation:
Rapid Commun. Mass Spectrom. 2009, 23 (2):258-266
Journal:
Rapid communications in mass spectrometry : RCM
Issue Date:
Jan-2009
URI:
http://hdl.handle.net/10146/83713
DOI:
10.1002/rcm.3873
PubMed ID:
19072863
Additional Links:
http://www3.interscience.wiley.com/journal/121561145/abstract?CRETRY=1&SRETRY=0
Type:
Article
Language:
en
ISSN:
0951-4198
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorTeichert, Friederikeen
dc.contributor.authorVerschoyle, Richard D.en
dc.contributor.authorGreaves, Peteren
dc.contributor.authorThorpe, James F.en
dc.contributor.authorMellon, J. Kilianen
dc.contributor.authorSteward, William P.en
dc.contributor.authorFarmer, Peter B.en
dc.contributor.authorGescher, Andreas J.en
dc.contributor.authorSingh, Rajinderen
dc.date.accessioned2009-10-07T07:25:25Z-
dc.date.available2009-10-07T07:25:25Z-
dc.date.issued2009-01-
dc.identifier.citationRapid Commun. Mass Spectrom. 2009, 23 (2):258-266en
dc.identifier.issn0951-4198-
dc.identifier.pmid19072863-
dc.identifier.doi10.1002/rcm.3873-
dc.identifier.urihttp://hdl.handle.net/10146/83713-
dc.description.abstractUrinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) represents a non-invasive biomarker for oxidative stress and may be useful for monitoring chemotherapeutic and chemopreventive interventions associated with cancer-related alterations in oxidative stress. We describe the development and validation of two separate liquid chromatography/tandem mass spectrometry (LC/MS/MS) selected reaction monitoring (SRM) methods for the determination of 8-oxodG and creatinine in both murine and human urine using stable isotope labelled internal standards. Levels of 8-oxodG were normalised to creatinine. The LC/MS/MS methods were applied to two chemoprevention studies utilising tea polyphenols in humans and TRAMP (TRansgenic Adenocarcinoma of the Mouse Prostate) mice. Patients with benign prostatic hyperplasia received 1 g/day of green tea polyphenols (GTP), 1 g/day of black tea theaflavins (BTT) or no treatment for 4 weeks. TRAMP mice received GTP (0.05% in drinking water) for 4 or 25 weeks. Prostate pathology in TRAMP mice was not affected by GTP. Levels of 8-oxodG were not altered by tea polyphenols in either mice or humans. In TRAMP mice, urinary 8-oxodG levels were elevated with increasing age (p < 0.0001) but not changed by the presence of prostate tumours. In conclusion, the LC/MS/MS SRM methods described here are ideally suited for the accurate determination of 8-oxodG and creatinine in urine samples from both clinical and pre-clinical studies.en
dc.language.isoenen
dc.relation.urlhttp://www3.interscience.wiley.com/journal/121561145/abstract?CRETRY=1&SRETRY=0en
dc.subjectAdenocarcinomaen
dc.subjectAntineoplastic Agentsen
dc.subjectChromatography, High Pressure Liquiden
dc.subjectCreatinineen
dc.subjectDeoxyguanosineen
dc.subjectPlant Extractsen
dc.subjectProstatic Neoplasmsen
dc.subjectSpectrometryen
dc.subjectTeaen
dc.subject.meshAdenocarcinoma-
dc.subject.meshAnimals-
dc.subject.meshAntineoplastic Agents-
dc.subject.meshCell Line, Tumor-
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshCreatinine-
dc.subject.meshDeoxyguanosine-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred C57BL-
dc.subject.meshPlant Extracts-
dc.subject.meshProstatic Neoplasms-
dc.subject.meshReproducibility of Results-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshSpectrometry, Mass, Electrospray Ionization-
dc.subject.meshTea-
dc.subject.meshUrinalysis-
dc.titleDetermination of 8-oxo-2'-deoxyguanosine and creatinine in murine and human urine by liquid chromatography/tandem mass spectrometry: application to chemoprevention studies.en
dc.typeArticleen
dc.identifier.journalRapid communications in mass spectrometry : RCMen
All Items in ECNIS-NIOM are protected by copyright, with all rights reserved, unless otherwise indicated.