Small field radiotherapy of head and neck cancer patients is responsible for oxidatively damaged DNA/oxidative stress on the level of a whole organism.

2.50
Hdl Handle:
http://hdl.handle.net/10146/83613
Title:
Small field radiotherapy of head and neck cancer patients is responsible for oxidatively damaged DNA/oxidative stress on the level of a whole organism.
Authors:
Roszkowski, Krzysztof; Gackowski, Daniel; Rozalski, Rafal; Dziaman, Tomasz; Siomek, Agnieszka; Guz, Jolanta; Szpila, Anna; Foksinski, Marek; Olinski, Ryszard
Abstract:
It is possible that oxidatively damaged DNA which arises as a result of radiotherapy may be involved in the therapeutic effect of the ionizing radiation and in the side effects. Therefore, for the first time, the broad spectrum of oxidatively damaged DNA biomarkers: urinary excretion of 8-oxodG (8-oxo-7,8-dihydro-2'-deoxyguanosine), 8-oxoGua (8-oxo-7,8-dihydroguanine) as well as the level of oxidatively damaged DNA in leukocytes, was analyzed in head and neck cancer patients (n = 27) undergoing fractionated radiotherapy using methodologies which involve HPLC (high-performance liquid chromatography) prepurification followed by gas chromatography with isotope dilution mass spectrometry detection and HPLC/EC. Of all the analyzed parameters in the majority of patients, only urinary excretion of the modified nucleoside significantly increased over the initial level in the samples collected 24 hr after the last fraction. However, for the distinct subpopulation of 10 patients, a significant increase in the level of 8-oxodG in cellular DNA and a simultaneous drop in urinary 8-oxoGua (the repair product of oxidative DNA damage) were detected after completion of the therapy. Because 8-oxoGua is a repair product of the DNA damage, there is a possibility that, at least in the case of some patients with the lowest activity of OGG1 (8-oxo-7,8-dihydroguanine glycosylase), the combination of lower OGG1 repair efficacy and irradiation was associated with increased background level of 8-oxoGua in cellular DNA. Apparently reduced DNA repair is unable to cope with the radiation-induced, and the extra amount of 8-oxoGua leading to an increase of potentially mutagenic/carcinogenic lesions.
Citation:
Int. J. Cancer 2008, 123 (8):1964-1967
Journal:
International journal of cancer. Journal international du cancer
Issue Date:
15-Oct-2008
URI:
http://hdl.handle.net/10146/83613
DOI:
10.1002/ijc.23700
PubMed ID:
18688851
Additional Links:
http://www3.interscience.wiley.com/journal/121359650/abstract?CRETRY=1&SRETRY=0
Type:
Article
Language:
en
ISSN:
1097-0215
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorRoszkowski, Krzysztofen
dc.contributor.authorGackowski, Danielen
dc.contributor.authorRozalski, Rafalen
dc.contributor.authorDziaman, Tomaszen
dc.contributor.authorSiomek, Agnieszkaen
dc.contributor.authorGuz, Jolantaen
dc.contributor.authorSzpila, Annaen
dc.contributor.authorFoksinski, Mareken
dc.contributor.authorOlinski, Ryszarden
dc.date.accessioned2009-10-06T10:35:49Z-
dc.date.available2009-10-06T10:35:49Z-
dc.date.issued2008-10-15-
dc.identifier.citationInt. J. Cancer 2008, 123 (8):1964-1967en
dc.identifier.issn1097-0215-
dc.identifier.pmid18688851-
dc.identifier.doi10.1002/ijc.23700-
dc.identifier.urihttp://hdl.handle.net/10146/83613-
dc.description.abstractIt is possible that oxidatively damaged DNA which arises as a result of radiotherapy may be involved in the therapeutic effect of the ionizing radiation and in the side effects. Therefore, for the first time, the broad spectrum of oxidatively damaged DNA biomarkers: urinary excretion of 8-oxodG (8-oxo-7,8-dihydro-2'-deoxyguanosine), 8-oxoGua (8-oxo-7,8-dihydroguanine) as well as the level of oxidatively damaged DNA in leukocytes, was analyzed in head and neck cancer patients (n = 27) undergoing fractionated radiotherapy using methodologies which involve HPLC (high-performance liquid chromatography) prepurification followed by gas chromatography with isotope dilution mass spectrometry detection and HPLC/EC. Of all the analyzed parameters in the majority of patients, only urinary excretion of the modified nucleoside significantly increased over the initial level in the samples collected 24 hr after the last fraction. However, for the distinct subpopulation of 10 patients, a significant increase in the level of 8-oxodG in cellular DNA and a simultaneous drop in urinary 8-oxoGua (the repair product of oxidative DNA damage) were detected after completion of the therapy. Because 8-oxoGua is a repair product of the DNA damage, there is a possibility that, at least in the case of some patients with the lowest activity of OGG1 (8-oxo-7,8-dihydroguanine glycosylase), the combination of lower OGG1 repair efficacy and irradiation was associated with increased background level of 8-oxoGua in cellular DNA. Apparently reduced DNA repair is unable to cope with the radiation-induced, and the extra amount of 8-oxoGua leading to an increase of potentially mutagenic/carcinogenic lesions.en
dc.language.isoenen
dc.relation.urlhttp://www3.interscience.wiley.com/journal/121359650/abstract?CRETRY=1&SRETRY=0en
dc.subjectradiotherapyen
dc.subjectoxidatively damaged DNAen
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshDNA Damage-
dc.subject.meshDNA, Neoplasm-
dc.subject.meshDeoxyguanosine-
dc.subject.meshDose Fractionation-
dc.subject.meshGas Chromatography-Mass Spectrometry-
dc.subject.meshGuanine-
dc.subject.meshHead and Neck Neoplasms-
dc.subject.meshHumans-
dc.subject.meshLeukocytes-
dc.subject.meshOxidative Stress-
dc.subject.meshRadiation Injuries-
dc.subject.meshUric Acid-
dc.titleSmall field radiotherapy of head and neck cancer patients is responsible for oxidatively damaged DNA/oxidative stress on the level of a whole organism.en
dc.typeArticleen
dc.identifier.journalInternational journal of cancer. Journal international du canceren

Related articles on PubMed

All Items in ECNIS-NIOM are protected by copyright, with all rights reserved, unless otherwise indicated.