A short-term intervention trial with selenate, selenium-enriched yeast and selenium-enriched milk: effects on oxidative defence regulation.

2.50
Hdl Handle:
http://hdl.handle.net/10146/82639
Title:
A short-term intervention trial with selenate, selenium-enriched yeast and selenium-enriched milk: effects on oxidative defence regulation.
Authors:
Ravn-Haren, Gitte; Bugel, Susanne; Krath, Britta N.; Hoac, Tien; Stagsted, Jan; Jorgensen, Karina; Bresson, June R.; Larsen, Erik H.; Dragsted, Lars O.
Abstract:
Increased Se intakes have been associated with decreased risk of cancer and CVD. Several mechanisms have been proposed, including antioxidant effects through selenoproteins, induction of carcinogen metabolism and effects on the blood lipid profile. In a 4 x 1 week randomised, double-blind cross-over study, healthy young men supplemented their usual diet with selenate, Se-enriched yeast, Se-enriched milk or placebo (Se dose was 300 microg/d for selenate and Se-enriched yeast, and about 480 microg/d for Se-enriched milk) followed by 8-week washout periods. All Se sources increased serum Se levels after supplementation for 1 week. The effect of the organic forms did not differ significantly and both increased serum Se more than selenate. Conversely, thrombocyte glutathione peroxidase (GPX) was increased in the periods where subjects were supplemented with selenate but not in those where they were given Se-enriched yeast or Se-enriched milk. We found no effect on plasma lipid resistance to oxidation, total cholesterol, TAG, HDL- and LDL-cholesterol, GPX, glutathione reductase (GR) and glutathione S-transferase (GST) activities measured in erythrocytes, GPX and GR activities determined in plasma, or GR and GST activities in thrombocytes. Leucocyte expression of genes encoding selenoproteins (GPX1, TrR1 and SelP), and of electrophile response element-regulated genes (GCLC, Fra1 and NQO1) were likewise unaffected at all time points following intervention. We conclude that thrombocyte GPX is specifically increased by short-term selenate supplementation, but not by short-term supplementation with organic Se. Short-term Se supplementation does not seem to affect blood lipid markers or expression and activity of selected enzymes and a transcription factor involved in glutathione-mediated detoxification and antioxidation.
Citation:
Br. J. Nutr. 2008, 99 (4):883-892
Journal:
The British journal of nutrition
Issue Date:
Apr-2008
URI:
http://hdl.handle.net/10146/82639
DOI:
10.1017/S0007114507825153
PubMed ID:
17888202
Additional Links:
http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1789632
Type:
Article
Language:
en
ISSN:
0007-1145
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorRavn-Haren, Gitteen
dc.contributor.authorBugel, Susanneen
dc.contributor.authorKrath, Britta N.en
dc.contributor.authorHoac, Tienen
dc.contributor.authorStagsted, Janen
dc.contributor.authorJorgensen, Karinaen
dc.contributor.authorBresson, June R.en
dc.contributor.authorLarsen, Erik H.en
dc.contributor.authorDragsted, Lars O.en
dc.date.accessioned2009-09-25T08:30:34Z-
dc.date.available2009-09-25T08:30:34Z-
dc.date.issued2008-04-
dc.identifier.citationBr. J. Nutr. 2008, 99 (4):883-892en
dc.identifier.issn0007-1145-
dc.identifier.pmid17888202-
dc.identifier.doi10.1017/S0007114507825153-
dc.identifier.urihttp://hdl.handle.net/10146/82639-
dc.description.abstractIncreased Se intakes have been associated with decreased risk of cancer and CVD. Several mechanisms have been proposed, including antioxidant effects through selenoproteins, induction of carcinogen metabolism and effects on the blood lipid profile. In a 4 x 1 week randomised, double-blind cross-over study, healthy young men supplemented their usual diet with selenate, Se-enriched yeast, Se-enriched milk or placebo (Se dose was 300 microg/d for selenate and Se-enriched yeast, and about 480 microg/d for Se-enriched milk) followed by 8-week washout periods. All Se sources increased serum Se levels after supplementation for 1 week. The effect of the organic forms did not differ significantly and both increased serum Se more than selenate. Conversely, thrombocyte glutathione peroxidase (GPX) was increased in the periods where subjects were supplemented with selenate but not in those where they were given Se-enriched yeast or Se-enriched milk. We found no effect on plasma lipid resistance to oxidation, total cholesterol, TAG, HDL- and LDL-cholesterol, GPX, glutathione reductase (GR) and glutathione S-transferase (GST) activities measured in erythrocytes, GPX and GR activities determined in plasma, or GR and GST activities in thrombocytes. Leucocyte expression of genes encoding selenoproteins (GPX1, TrR1 and SelP), and of electrophile response element-regulated genes (GCLC, Fra1 and NQO1) were likewise unaffected at all time points following intervention. We conclude that thrombocyte GPX is specifically increased by short-term selenate supplementation, but not by short-term supplementation with organic Se. Short-term Se supplementation does not seem to affect blood lipid markers or expression and activity of selected enzymes and a transcription factor involved in glutathione-mediated detoxification and antioxidation.en
dc.language.isoenen
dc.relation.urlhttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1789632en
dc.subjectSeleniumen
dc.subjectOxidative defenceen
dc.subjectElectrophile response elementsen
dc.subjectGlutathione peroxidaseen
dc.subjectBlood lipidsen
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAnimals-
dc.subject.meshAntioxidants-
dc.subject.meshBase Sequence-
dc.subject.meshBiological Markers-
dc.subject.meshC-Reactive Protein-
dc.subject.meshCholesterol-
dc.subject.meshCholesterol, HDL-
dc.subject.meshCholesterol, LDL-
dc.subject.meshCross-Over Studies-
dc.subject.meshDNA Primers-
dc.subject.meshDietary Supplements-
dc.subject.meshDouble-Blind Method-
dc.subject.meshFood, Fortified-
dc.subject.meshGene Expression-
dc.subject.meshHumans-
dc.subject.meshLeukocytes-
dc.subject.meshLipids-
dc.subject.meshMale-
dc.subject.meshMilk-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshOxidation-Reduction-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshSelenium-
dc.subject.meshYeast, Dried-
dc.titleA short-term intervention trial with selenate, selenium-enriched yeast and selenium-enriched milk: effects on oxidative defence regulation.en
dc.typeArticleen
dc.identifier.journalThe British journal of nutritionen
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