2.50
Hdl Handle:
http://hdl.handle.net/10146/77962
Title:
Biomarkers of oxidative damage to DNA and repair.
Authors:
Loft, Steffen; Hogh Danielsen, Pernille; Mikkelsen, Lone; Risom, Lotte; Forchhammer, Lykke; Moller, Peter
Abstract:
Oxidative-stress-induced damage to DNA includes a multitude of lesions, many of which are mutagenic and have multiple roles in cancer and aging. Many lesions have been characterized by MS-based methods after extraction and digestion of DNA. These preparation steps may cause spurious base oxidation, which is less likely to occur with methods such as the comet assay, which are based on nicking of the DNA strand at modified bases, but offer less specificity. The European Standards Committee on Oxidative DNA Damage has concluded that the true levels of the most widely studied lesion, 8-oxodG (8-oxo-7,8-dihydro-2'-deoxyguanosine), in cellular DNA is between 0.5 and 5 lesions per 10(6) dG bases. Base excision repair of oxidative damage to DNA can be assessed by nicking assays based on oligonucleotides with lesions or the comet assay, by mRNA expression levels or, in the case of, e.g., OGG1 (8-oxoguanine DNA glycosylase 1), responsible for repair of 8-oxodG, by genotyping. Products of repair in DNA or the nucleotide pool, such as 8-oxodG, excreted into the urine can be assessed by MS-based methods and generally reflects the rate of damage. Experimental and population-based studies indicate that many environmental factors, including particulate air pollution, cause oxidative damage to DNA, whereas diets rich in fruit and vegetables or antioxidant supplements may reduce the levels and enhance repair. Urinary excretion of 8-oxodG, genotype and expression of OGG1 have been associated with risk of cancer in cohort settings, whereas altered levels of damage, repair or urinary excretion in case-control settings may be a consequence rather than the cause of the disease.
Citation:
Biochem. Soc. Trans. 2008, 36 (Pt 5):1071-1076
Journal:
Biochemical Society transactions
Issue Date:
Oct-2008
URI:
http://hdl.handle.net/10146/77962
DOI:
10.1042/BST0361071
PubMed ID:
18793191
Additional Links:
http://www.biochemsoctrans.org/bst/036/bst0361071.htm
Type:
Article
Language:
en
ISSN:
0300-5127
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorLoft, Steffen-
dc.contributor.authorHogh Danielsen, Pernille-
dc.contributor.authorMikkelsen, Lone-
dc.contributor.authorRisom, Lotte-
dc.contributor.authorForchhammer, Lykke-
dc.contributor.authorMoller, Peter-
dc.date.accessioned2009-08-20T11:23:12Z-
dc.date.available2009-08-20T11:23:12Z-
dc.date.issued2008-10-
dc.identifier.citationBiochem. Soc. Trans. 2008, 36 (Pt 5):1071-1076en
dc.identifier.issn0300-5127-
dc.identifier.pmid18793191-
dc.identifier.doi10.1042/BST0361071-
dc.identifier.urihttp://hdl.handle.net/10146/77962-
dc.description.abstractOxidative-stress-induced damage to DNA includes a multitude of lesions, many of which are mutagenic and have multiple roles in cancer and aging. Many lesions have been characterized by MS-based methods after extraction and digestion of DNA. These preparation steps may cause spurious base oxidation, which is less likely to occur with methods such as the comet assay, which are based on nicking of the DNA strand at modified bases, but offer less specificity. The European Standards Committee on Oxidative DNA Damage has concluded that the true levels of the most widely studied lesion, 8-oxodG (8-oxo-7,8-dihydro-2'-deoxyguanosine), in cellular DNA is between 0.5 and 5 lesions per 10(6) dG bases. Base excision repair of oxidative damage to DNA can be assessed by nicking assays based on oligonucleotides with lesions or the comet assay, by mRNA expression levels or, in the case of, e.g., OGG1 (8-oxoguanine DNA glycosylase 1), responsible for repair of 8-oxodG, by genotyping. Products of repair in DNA or the nucleotide pool, such as 8-oxodG, excreted into the urine can be assessed by MS-based methods and generally reflects the rate of damage. Experimental and population-based studies indicate that many environmental factors, including particulate air pollution, cause oxidative damage to DNA, whereas diets rich in fruit and vegetables or antioxidant supplements may reduce the levels and enhance repair. Urinary excretion of 8-oxodG, genotype and expression of OGG1 have been associated with risk of cancer in cohort settings, whereas altered levels of damage, repair or urinary excretion in case-control settings may be a consequence rather than the cause of the disease.en
dc.language.isoenen
dc.relation.urlhttp://www.biochemsoctrans.org/bst/036/bst0361071.htmen
dc.subjectbase excision repairen
dc.subjectbiomarkeren
dc.subjectDNA damageen
dc.subjectDNA repairen
dc.subjectnucleotide excision repairen
dc.subjectoxidative damageen
dc.subject.meshBiological Markers-
dc.subject.meshDNA Damage-
dc.subject.meshDNA Repair-
dc.subject.meshDiet-
dc.subject.meshDietary Supplements-
dc.subject.meshEnvironment-
dc.subject.meshHumans-
dc.subject.meshMolecular Structure-
dc.subject.meshNeoplasms-
dc.subject.meshOxidation-Reduction-
dc.subject.meshOxidative Stress-
dc.titleBiomarkers of oxidative damage to DNA and repair.en
dc.typeArticleen
dc.identifier.journalBiochemical Society transactionsen

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