Down-regulation of oxidative DNA lesions in human mononuclear cells after antioxidant supplementation correlates to increase of gamma-tocopherol.

2.50
Hdl Handle:
http://hdl.handle.net/10146/76777
Title:
Down-regulation of oxidative DNA lesions in human mononuclear cells after antioxidant supplementation correlates to increase of gamma-tocopherol.
Authors:
Johansson, Clara; Rytter, Elisabet; Nygren, Jonas; Vessby, Bengt; Basu, Samar; Moller, Lennart
Abstract:
The protective effect of vitamin E supplements has been questioned, possibly because they often contain only alpha-tocopherol, and recent studies indicate that gamma-tocopherol also has important properties. The aim of this study was to investigate whether the levels of DNA lesions in middle-aged, overweight males could be reduced by consumption of low doses of an antioxidant supplement for six weeks, designed to imitate a balanced diet. The participants (n=60) were randomly divided into: placebo, single-, and double-dose groups. Genotoxic and oxidative DNA lesions in mononuclear cells were measured with the Comet assay, before and after supplement administration. Furthermore, a cell study was performed to investigate if pre-incubation of a human lung cell line (A549) with alpha- and gamma-tocopherol (5 and 50 microM for 23 hours) could protect against induced oxidative DNA lesions as measured by the Comet assay. The level of oxidative DNA lesions in the double-dose group was significantly lower than in the control group. Oxidative DNA lesions correlated only to changes in serum gamma-tocopherol, and not alpha-tocopherol. In the cell study, only gamma-tocopherol protected cells against induced oxidative DNA lesions. We therefore hypothesize that gamma-tocopheol rather than alpha-tocopherol is involved in reducing oxidative DNA lesions.
Citation:
Int. J. Vitam. Nutr. Res. 2008, 78 (4-5):183-194
Journal:
International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition
Issue Date:
10-Aug-2009
URI:
http://hdl.handle.net/10146/76777
DOI:
10.1024/0300-9831.78.45.183
PubMed ID:
19326341
Additional Links:
http://www.verlag-hanshuber.com/zeitschriften/journal.php?abbrev=VIT&show=abstract&abstract=24885
Type:
Article
Language:
en
ISSN:
0300-9831
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorJohansson, Clara-
dc.contributor.authorRytter, Elisabet-
dc.contributor.authorNygren, Jonas-
dc.contributor.authorVessby, Bengt-
dc.contributor.authorBasu, Samar-
dc.contributor.authorMoller, Lennart-
dc.date.accessioned2009-08-10T09:12:28Z-
dc.date.available2009-08-10T09:12:28Z-
dc.date.issued2009-08-10T09:12:28Z-
dc.identifier.citationInt. J. Vitam. Nutr. Res. 2008, 78 (4-5):183-194en
dc.identifier.issn0300-9831-
dc.identifier.pmid19326341-
dc.identifier.doi10.1024/0300-9831.78.45.183-
dc.identifier.urihttp://hdl.handle.net/10146/76777-
dc.description.abstractThe protective effect of vitamin E supplements has been questioned, possibly because they often contain only alpha-tocopherol, and recent studies indicate that gamma-tocopherol also has important properties. The aim of this study was to investigate whether the levels of DNA lesions in middle-aged, overweight males could be reduced by consumption of low doses of an antioxidant supplement for six weeks, designed to imitate a balanced diet. The participants (n=60) were randomly divided into: placebo, single-, and double-dose groups. Genotoxic and oxidative DNA lesions in mononuclear cells were measured with the Comet assay, before and after supplement administration. Furthermore, a cell study was performed to investigate if pre-incubation of a human lung cell line (A549) with alpha- and gamma-tocopherol (5 and 50 microM for 23 hours) could protect against induced oxidative DNA lesions as measured by the Comet assay. The level of oxidative DNA lesions in the double-dose group was significantly lower than in the control group. Oxidative DNA lesions correlated only to changes in serum gamma-tocopherol, and not alpha-tocopherol. In the cell study, only gamma-tocopherol protected cells against induced oxidative DNA lesions. We therefore hypothesize that gamma-tocopheol rather than alpha-tocopherol is involved in reducing oxidative DNA lesions.en
dc.language.isoenen
dc.relation.urlhttp://www.verlag-hanshuber.com/zeitschriften/journal.php?abbrev=VIT&show=abstract&abstract=24885en
dc.subjectantioxidant supplementsen
dc.subjectComet assayen
dc.subjectDNA damageen
dc.subjectDNA lesionsen
dc.subjectoxidative stressen
dc.subjecttocopherolen
dc.subjectVitamin Een
dc.subject.meshAdult-
dc.subject.meshAngiosperms-
dc.subject.meshAntioxidants-
dc.subject.meshCell Line-
dc.subject.meshComet Assay-
dc.subject.meshDNA Damage-
dc.subject.meshDietary Supplements-
dc.subject.meshDown-Regulation-
dc.subject.meshFruit-
dc.subject.meshHumans-
dc.subject.meshLeukocytes, Mononuclear-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshOverweight-
dc.subject.meshPhytotherapy-
dc.subject.meshPlant Extracts-
dc.subject.meshVegetables-
dc.subject.meshalpha-Tocopherol-
dc.subject.meshgamma-Tocopherol-
dc.titleDown-regulation of oxidative DNA lesions in human mononuclear cells after antioxidant supplementation correlates to increase of gamma-tocopherol.en
dc.typeArticleen
dc.identifier.journalInternational journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutritionen

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