Validation of biomarkers for the study of environmental carcinogens: a review.

2.50
Hdl Handle:
http://hdl.handle.net/10146/76633
Title:
Validation of biomarkers for the study of environmental carcinogens: a review.
Authors:
Gallo, Valentina; Khan, Aneire; Gonzales, Carlos; Phillips, David H.; Schoket, Bernadette; Gyorffy, Erika; Anna, Livia; Kovacs, Katalin; Moller, Peter; Loft, Steffen; Kyrtopoulos, Soterios; Matullo, Giuseppe; Vineis, Paolo
Abstract:
There is a need for validation of biomarkers. Our aim is to review published work on the validation of selected biomarkers: bulky DNA adducts, N-nitroso compounds, 1-hydroxypyrene, and oxidative damage to DNA. A systematic literature search in PubMed was performed. Information on the variability and reliability of the laboratory tests used for biomarkers measurements was collected. For the evaluation of the evidence on validation we referred to the ACCE criteria. Little is known about intraindividual variation of DNA adduct measurements, but measurements have a good repeatability irrespective of the technique used for their identification; reproducibility improved after the correction for a laboratory factor. A high-sensitivity method is available for the measurement of 1-hydroxypyrene in urine. There is consensus on validation of biomarkers of oxidative damage DNA based on the comet assay and chromatographic measurement in blood while urinary measurements by chromatographic assays are well validated, and ELISA-based assays appear to lack specificity. Immunoassays for the quantification of adducts of N-nitroso compounds are useful for large epidemiological studies, given their sensitivity, the small amount of DNA required and their potential for rapid and high-throughput analysis.
Citation:
Biomarkers 2008, 13 (5):505-534
Journal:
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals
Issue Date:
Aug-2008
URI:
http://hdl.handle.net/10146/76633
PubMed ID:
18979642
Additional Links:
http://www.informaworld.com/smpp/content~db=all~content=a792165638
Type:
Article
Language:
en
ISSN:
1354-750X
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorGallo, Valentina-
dc.contributor.authorKhan, Aneire-
dc.contributor.authorGonzales, Carlos-
dc.contributor.authorPhillips, David H.-
dc.contributor.authorSchoket, Bernadette-
dc.contributor.authorGyorffy, Erika-
dc.contributor.authorAnna, Livia-
dc.contributor.authorKovacs, Katalin-
dc.contributor.authorMoller, Peter-
dc.contributor.authorLoft, Steffen-
dc.contributor.authorKyrtopoulos, Soterios-
dc.contributor.authorMatullo, Giuseppe-
dc.contributor.authorVineis, Paolo-
dc.date.accessioned2009-08-07T07:45:14Z-
dc.date.available2009-08-07T07:45:14Z-
dc.date.issued2008-08-
dc.identifier.citationBiomarkers 2008, 13 (5):505-534en
dc.identifier.issn1354-750X-
dc.identifier.pmid18979642-
dc.identifier.urihttp://hdl.handle.net/10146/76633-
dc.description.abstractThere is a need for validation of biomarkers. Our aim is to review published work on the validation of selected biomarkers: bulky DNA adducts, N-nitroso compounds, 1-hydroxypyrene, and oxidative damage to DNA. A systematic literature search in PubMed was performed. Information on the variability and reliability of the laboratory tests used for biomarkers measurements was collected. For the evaluation of the evidence on validation we referred to the ACCE criteria. Little is known about intraindividual variation of DNA adduct measurements, but measurements have a good repeatability irrespective of the technique used for their identification; reproducibility improved after the correction for a laboratory factor. A high-sensitivity method is available for the measurement of 1-hydroxypyrene in urine. There is consensus on validation of biomarkers of oxidative damage DNA based on the comet assay and chromatographic measurement in blood while urinary measurements by chromatographic assays are well validated, and ELISA-based assays appear to lack specificity. Immunoassays for the quantification of adducts of N-nitroso compounds are useful for large epidemiological studies, given their sensitivity, the small amount of DNA required and their potential for rapid and high-throughput analysis.en
dc.language.isoenen
dc.relation.urlhttp://www.informaworld.com/smpp/content~db=all~content=a792165638en
dc.subjectValidationen
dc.subjectDNA adductsen
dc.subject1-hydroxypyreneen
dc.subjectoxidative damage to DNAen
dc.subjectN-nitroso compoundsen
dc.subjectenvironmental carcinogenesisen
dc.subject.meshBiological Markers-
dc.subject.meshCarcinogens, Environmental-
dc.subject.meshDNA Adducts-
dc.subject.meshDNA Damage-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshEnzyme-Linked Immunosorbent Assay-
dc.subject.meshHumans-
dc.subject.meshOxidative Stress-
dc.titleValidation of biomarkers for the study of environmental carcinogens: a review.en
dc.typeArticleen
dc.identifier.journalBiomarkers : biochemical indicators of exposure, response, and susceptibility to chemicalsen
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