Oxidatively damaged DNA in rats exposed by oral gavage to C60 fullerenes and single-walled carbon nanotubes.

4.00
Hdl Handle:
http://hdl.handle.net/10146/76615
Title:
Oxidatively damaged DNA in rats exposed by oral gavage to C60 fullerenes and single-walled carbon nanotubes.
Authors:
Folkmann, Janne K.; Risom, Lotte; Jacobsen, Nicklas R.; Wallin, Hakan; Loft, Steffen; Moller, Peter
Abstract:
BACKGROUND: C60 fullerenes and single-walled carbon nanotubes (SWCNT) are projected to be used in medicine and consumer products with potential human exposure. The hazardous effects of these particles are expected to involve oxidative stress with generation of oxidatively damaged DNA that might be the initiating event in the development of cancer. OBJECTIVE: In this study we investigated the effect of a single oral administration of C60 fullerenes and SWCNT. METHODS: We measured the level of oxidative damage to DNA as the premutagenic 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in the colon mucosa, liver, and lung of rats after intragastric administration of pristine C60 fullerenes or SWCNT (0.064 or 0.64 mg/kg body weight) suspended in saline solution or corn oil. We investigated the regulation of DNA repair systems toward 8-oxodG in liver and lung tissue. RESULTS: Both doses of SWCNT increased the levels of 8-oxodG in liver and lung. Administration of C60 fullerenes increased the hepatic level of 8-oxodG, whereas only the high dose generated 8-oxodG in the lung. We detected no effects on 8-oxodG in colon mucosa. Suspension of particles in saline solution or corn oil yielded a similar extent of genotoxicity, whereas corn oil per se generated more genotoxicity than the particles. Although there was increased mRNA expression of 8-oxoguanine DNA glycosylase in the liver of C60 fullerene-treated rats, we found no significant increase in repair activity. CONCLUSIONS: Oral exposure to low doses of C60 fullerenes and SWCNT is associated with elevated levels of 8-oxodG in the liver and lung, which is likely to be caused by a direct genotoxic ability rather than an inhibition of the DNA repair system.
Citation:
Environ. Health Perspect. 2009, 117 (5):703-708
Journal:
Environmental health perspectives
Issue Date:
May-2009
URI:
http://hdl.handle.net/10146/76615
DOI:
10.1289/ehp.11922
PubMed ID:
19479010
Additional Links:
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=19479010; http://ukpmc.ac.uk/articlerender.cgi?tool=pubmed&pubmedid=19479010
Type:
Article
Language:
en
ISSN:
1552-9924
Sponsors:
This study was supported by grants from the Research Centre for Environmental Health, the Danish Research Councils, and the European Union (grant FP6-012912, NEST, Particle Risk) and ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility) a network of excellence operating within the European Union Sixth Framework Program, Priority 5: Food Quality and Safety (contract 513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorFolkmann, Janne K.-
dc.contributor.authorRisom, Lotte-
dc.contributor.authorJacobsen, Nicklas R.-
dc.contributor.authorWallin, Hakan-
dc.contributor.authorLoft, Steffen-
dc.contributor.authorMoller, Peter-
dc.date.accessioned2009-08-07T07:15:14Z-
dc.date.available2009-08-07T07:15:14Z-
dc.date.issued2009-05-
dc.identifier.citationEnviron. Health Perspect. 2009, 117 (5):703-708en
dc.identifier.issn1552-9924-
dc.identifier.pmid19479010-
dc.identifier.doi10.1289/ehp.11922-
dc.identifier.urihttp://hdl.handle.net/10146/76615-
dc.description.abstractBACKGROUND: C60 fullerenes and single-walled carbon nanotubes (SWCNT) are projected to be used in medicine and consumer products with potential human exposure. The hazardous effects of these particles are expected to involve oxidative stress with generation of oxidatively damaged DNA that might be the initiating event in the development of cancer. OBJECTIVE: In this study we investigated the effect of a single oral administration of C60 fullerenes and SWCNT. METHODS: We measured the level of oxidative damage to DNA as the premutagenic 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in the colon mucosa, liver, and lung of rats after intragastric administration of pristine C60 fullerenes or SWCNT (0.064 or 0.64 mg/kg body weight) suspended in saline solution or corn oil. We investigated the regulation of DNA repair systems toward 8-oxodG in liver and lung tissue. RESULTS: Both doses of SWCNT increased the levels of 8-oxodG in liver and lung. Administration of C60 fullerenes increased the hepatic level of 8-oxodG, whereas only the high dose generated 8-oxodG in the lung. We detected no effects on 8-oxodG in colon mucosa. Suspension of particles in saline solution or corn oil yielded a similar extent of genotoxicity, whereas corn oil per se generated more genotoxicity than the particles. Although there was increased mRNA expression of 8-oxoguanine DNA glycosylase in the liver of C60 fullerene-treated rats, we found no significant increase in repair activity. CONCLUSIONS: Oral exposure to low doses of C60 fullerenes and SWCNT is associated with elevated levels of 8-oxodG in the liver and lung, which is likely to be caused by a direct genotoxic ability rather than an inhibition of the DNA repair system.en
dc.description.sponsorshipThis study was supported by grants from the Research Centre for Environmental Health, the Danish Research Councils, and the European Union (grant FP6-012912, NEST, Particle Risk) and ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility) a network of excellence operating within the European Union Sixth Framework Program, Priority 5: Food Quality and Safety (contract 513943).en
dc.language.isoenen
dc.relation.urlhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=19479010en
dc.relation.urlhttp://ukpmc.ac.uk/articlerender.cgi?tool=pubmed&pubmedid=19479010en
dc.subjectcanceren
dc.subjectDNA damageen
dc.subjectoxidative stressen
dc.subjectDNA repairen
dc.subjectnanoparticleen
dc.subject.meshAnimalsen
dc.subject.meshColonen
dc.subject.meshDNA Damageen
dc.subject.meshDNA Glycosylasesen
dc.subject.meshDeoxyguanosineen
dc.subject.meshDrug Carriersen
dc.subject.meshFemaleen
dc.subject.meshFullerenesen
dc.subject.meshLiveren
dc.subject.meshLungen
dc.subject.meshNanotubes, Carbonen
dc.subject.meshOxidation-Reductionen
dc.subject.meshOxidative Stressen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshRatsen
dc.titleOxidatively damaged DNA in rats exposed by oral gavage to C60 fullerenes and single-walled carbon nanotubes.en
dc.typeArticleen
dc.identifier.journalEnvironmental health perspectivesen

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