Oxidative damage to DNA and repair induced by Norwegian wood smoke particles in human A549 and THP-1 cell lines.

2.50
Hdl Handle:
http://hdl.handle.net/10146/76317
Title:
Oxidative damage to DNA and repair induced by Norwegian wood smoke particles in human A549 and THP-1 cell lines.
Authors:
Danielsen, Pernille Hogh; Loft, Steffen; Kocbach, Anette; Schwarze, Per E.; Moller, Peter
Abstract:
Genotoxic effects of traffic-generated particulate matter (PM) are well described, whereas little data are available on PM from combustion of biomass and wood, which contributes substantially to air pollution world wide. The aim of this study was to compare the genotoxicity of wood smoke particulate matter (WSPM), authentic traffic-generated particles, mineral PM and standard reference material (SRM2975) of diesel exhaust particles in human A549 lung epithelial and THP-1 monocytic cell lines. DNA damage was measured as strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sites by the comet assay, whereas cell cytotoxicity was determined as lactate dehydrogenase release. The exposure to WSPM generated SB and FPG sites in both cell lines at concentrations from 2.5 or 25 microg/ml, which were not cytotoxic. Compared to all other studied particles, WSPM generated greater responses in terms of both SB and FPG sites. Organic extracts of WSPM and SRM2975 elicited higher levels of SB than native and washed PM at 25 and 100 microg/ml, whereas assay saturation precluded reliable assessment of FPG sites. During a 6h post-exposure period, in which the medium with PM had been replaced by fresh medium, 60% of the DNA lesions generated by WSPM were removed. In conclusion, WSPM generated more DNA damage than traffic-generated PM per unit mass in human cell lines, possibly due to the high level of polycyclic aromatic hydrocarbons in WSPM. This suggests that exposure to WSPM might be more hazardous than PM collected from vehicle exhaust with respect to development of lung cancer.
Citation:
Mutat. Res. 2009, 674 (1-2):116-122
Journal:
Mutation Research
Issue Date:
31-Mar-2009
URI:
http://hdl.handle.net/10146/76317
DOI:
10.1016/j.mrgentox.2008.10.014
PubMed ID:
19041418
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2D-4TWVX48-2&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=6c5559a5c30056e7044870c33b74a85e
Type:
Article
Language:
en
ISSN:
0027-5107
Sponsors:
Pernille Høgh Danielsen, Peter Møller and Steffen Loft are partners in ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No 513943).
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorDanielsen, Pernille Hogh-
dc.contributor.authorLoft, Steffen-
dc.contributor.authorKocbach, Anette-
dc.contributor.authorSchwarze, Per E.-
dc.contributor.authorMoller, Peter-
dc.date.accessioned2009-08-05T07:58:32Z-
dc.date.available2009-08-05T07:58:32Z-
dc.date.issued2009-03-31-
dc.identifier.citationMutat. Res. 2009, 674 (1-2):116-122en
dc.identifier.issn0027-5107-
dc.identifier.pmid19041418-
dc.identifier.doi10.1016/j.mrgentox.2008.10.014-
dc.identifier.urihttp://hdl.handle.net/10146/76317-
dc.description.abstractGenotoxic effects of traffic-generated particulate matter (PM) are well described, whereas little data are available on PM from combustion of biomass and wood, which contributes substantially to air pollution world wide. The aim of this study was to compare the genotoxicity of wood smoke particulate matter (WSPM), authentic traffic-generated particles, mineral PM and standard reference material (SRM2975) of diesel exhaust particles in human A549 lung epithelial and THP-1 monocytic cell lines. DNA damage was measured as strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sites by the comet assay, whereas cell cytotoxicity was determined as lactate dehydrogenase release. The exposure to WSPM generated SB and FPG sites in both cell lines at concentrations from 2.5 or 25 microg/ml, which were not cytotoxic. Compared to all other studied particles, WSPM generated greater responses in terms of both SB and FPG sites. Organic extracts of WSPM and SRM2975 elicited higher levels of SB than native and washed PM at 25 and 100 microg/ml, whereas assay saturation precluded reliable assessment of FPG sites. During a 6h post-exposure period, in which the medium with PM had been replaced by fresh medium, 60% of the DNA lesions generated by WSPM were removed. In conclusion, WSPM generated more DNA damage than traffic-generated PM per unit mass in human cell lines, possibly due to the high level of polycyclic aromatic hydrocarbons in WSPM. This suggests that exposure to WSPM might be more hazardous than PM collected from vehicle exhaust with respect to development of lung cancer.en
dc.description.sponsorshipPernille Høgh Danielsen, Peter Møller and Steffen Loft are partners in ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th Framework Program, Priority 5: “Food Quality and Safety” (Contract No 513943).en
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2D-4TWVX48-2&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=6c5559a5c30056e7044870c33b74a85een
dc.subjectComet assayen
dc.subjectDNA damageen
dc.subjectDNA repairen
dc.subjectOrganic extracten
dc.subjectOxidative stressen
dc.subjectStrand breaksen
dc.subjectWood smokeen
dc.subject.meshCell Line-
dc.subject.meshChemical Fractionation-
dc.subject.meshDNA Damage-
dc.subject.meshDNA Repair-
dc.subject.meshHumans-
dc.subject.meshL-Lactate Dehydrogenase-
dc.subject.meshOxidative Stress-
dc.subject.meshParticulate Matter-
dc.subject.meshSmoke-
dc.subject.meshWood-
dc.titleOxidative damage to DNA and repair induced by Norwegian wood smoke particles in human A549 and THP-1 cell lines.en
dc.typeArticleen
dc.identifier.journalMutation Researchen

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