Characterization of 67 kD laminin receptor, a protein whose gene is overexpressed on treatment of cells with anti-benzo[a]pyrene-7,8-diol-9,10-epoxide.

2.50
Hdl Handle:
http://hdl.handle.net/10146/68638
Title:
Characterization of 67 kD laminin receptor, a protein whose gene is overexpressed on treatment of cells with anti-benzo[a]pyrene-7,8-diol-9,10-epoxide.
Authors:
An, She-Juan; Chen, Jia-Kun; Chen, Hua-jie; Chang, Wei; Jiang, Yi-Guo; Wei, Qing-Yi; Chen, Xue-Min
Abstract:
The molecular mechanisms potentially related to tumorigenesis induced by anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (anti-BPDE) were investigated by suppression subtractive hybridization of the human bronchial epithelial cells (16HBE) carcinoma induced by BPDE-transformed 16HBE cells (16HBE-C). The 67 kD laminin receptor gene (67LR1) is one of the screened overexpressed genes in 16HBE-C cells when compared with 16HBE. In order to understand the main functions of 67LR1 gene, we amplified the full length of 67LR1 gene using reverse transcription-polymerase chain reaction (RT-PCR) method. The amplified gene products were inserted into pcDNA 3.1 Directional TOPO expression vector. We then transfected 16HBE cells with this vector and derived stable transfected 16HBE cell lines containing the 67LR1 gene by using lipofectin and G418 selection protocols. The expression products of transfected genes were analyzed by semiquantitative RT-PCR. Soft agar growth assay was carried out to identify the malignant features of 67LR1 gene. The stable transfected cell lines can form colonies in soft agar. Further, the transfected cells showed morphological changes compared to the control cells, such as the obvious pseudopods. These data suggest that the 67LR1 gene may be related to malignant transformation induced by the anti-BPDE. The 67LR1 protein may be related to the directionality of cell movement.
Citation:
Toxicol. Sci. 2006, 90 (2):326-330
Journal:
Toxicological sciences : an official journal of the Society of Toxicology
Issue Date:
Apr-2006
URI:
http://hdl.handle.net/10146/68638
DOI:
10.1093/toxsci/kfj109
PubMed ID:
16431845
Additional Links:
http://toxsci.oxfordjournals.org/cgi/content/full/90/2/326
Type:
Article
Language:
en
Description:
KEYWORDS - CLASSIFICATION: 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide;Animals;biosynthesis;Base Sequence;Carcinoma,Squamous Cell;Cell Line,Tumor;Cell Transformation,Neoplastic;China;drug effects;Environmental Health;Epithelial Cells;genetics;Gene Expression Regulation,Neoplastic;Humans;Laminin;metabolism;mechanisms of carcinogenesis;Mice;Mice,Nude;Molecular Sequence Data;Public Health;Receptors,Laminin;Research;Rna;Sequence Analysis,RNA;toxicity;Transfection;Tumor Markers,Biological.
ISSN:
1096-6080
Appears in Collections:
Articles with annotation

Full metadata record

DC FieldValue Language
dc.contributor.authorAn, She-Juan-
dc.contributor.authorChen, Jia-Kun-
dc.contributor.authorChen, Hua-jie-
dc.contributor.authorChang, Wei-
dc.contributor.authorJiang, Yi-Guo-
dc.contributor.authorWei, Qing-Yi-
dc.contributor.authorChen, Xue-Min-
dc.date.accessioned2009-05-20T10:56:21Z-
dc.date.available2009-05-20T10:56:21Z-
dc.date.issued2006-04-
dc.identifier.citationToxicol. Sci. 2006, 90 (2):326-330en
dc.identifier.issn1096-6080-
dc.identifier.pmid16431845-
dc.identifier.doi10.1093/toxsci/kfj109-
dc.identifier.urihttp://hdl.handle.net/10146/68638-
dc.descriptionKEYWORDS - CLASSIFICATION: 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide;Animals;biosynthesis;Base Sequence;Carcinoma,Squamous Cell;Cell Line,Tumor;Cell Transformation,Neoplastic;China;drug effects;Environmental Health;Epithelial Cells;genetics;Gene Expression Regulation,Neoplastic;Humans;Laminin;metabolism;mechanisms of carcinogenesis;Mice;Mice,Nude;Molecular Sequence Data;Public Health;Receptors,Laminin;Research;Rna;Sequence Analysis,RNA;toxicity;Transfection;Tumor Markers,Biological.en
dc.description.abstractThe molecular mechanisms potentially related to tumorigenesis induced by anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (anti-BPDE) were investigated by suppression subtractive hybridization of the human bronchial epithelial cells (16HBE) carcinoma induced by BPDE-transformed 16HBE cells (16HBE-C). The 67 kD laminin receptor gene (67LR1) is one of the screened overexpressed genes in 16HBE-C cells when compared with 16HBE. In order to understand the main functions of 67LR1 gene, we amplified the full length of 67LR1 gene using reverse transcription-polymerase chain reaction (RT-PCR) method. The amplified gene products were inserted into pcDNA 3.1 Directional TOPO expression vector. We then transfected 16HBE cells with this vector and derived stable transfected 16HBE cell lines containing the 67LR1 gene by using lipofectin and G418 selection protocols. The expression products of transfected genes were analyzed by semiquantitative RT-PCR. Soft agar growth assay was carried out to identify the malignant features of 67LR1 gene. The stable transfected cell lines can form colonies in soft agar. Further, the transfected cells showed morphological changes compared to the control cells, such as the obvious pseudopods. These data suggest that the 67LR1 gene may be related to malignant transformation induced by the anti-BPDE. The 67LR1 protein may be related to the directionality of cell movement.en
dc.language.isoenen
dc.relation.urlhttp://toxsci.oxfordjournals.org/cgi/content/full/90/2/326en
dc.subject.mesh7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide-
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshCarcinoma, Squamous Cell-
dc.subject.meshCell Line, Tumor-
dc.subject.meshCell Transformation, Neoplastic-
dc.subject.meshEpithelial Cells-
dc.subject.meshGene Expression Regulation, Neoplastic-
dc.subject.meshHumans-
dc.subject.meshMice-
dc.subject.meshMice, Nude-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshRNA-
dc.subject.meshReceptors, Laminin-
dc.subject.meshSequence Analysis, RNA-
dc.subject.meshTransfection-
dc.subject.meshTumor Markers, Biological-
dc.titleCharacterization of 67 kD laminin receptor, a protein whose gene is overexpressed on treatment of cells with anti-benzo[a]pyrene-7,8-diol-9,10-epoxide.en
dc.typeArticleen
dc.identifier.journalToxicological sciences : an official journal of the Society of Toxicologyen

Related articles on PubMed

All Items in ECNIS-NIOM are protected by copyright, with all rights reserved, unless otherwise indicated.