ROS release and Hsp70 expression after exposure to 1,800 MHz radiofrequency electromagnetic fields in primary human monocytes and lymphocytes.

2.50
Hdl Handle:
http://hdl.handle.net/10146/64996
Title:
ROS release and Hsp70 expression after exposure to 1,800 MHz radiofrequency electromagnetic fields in primary human monocytes and lymphocytes.
Authors:
Lantow, M.; Lupke, M.; Frahm, J.; Mattsson, M. O.; Kuster, N.; Simko, M.
Abstract:
The aim of this study is to investigate if 1,800 MHz radiofrequency electromagnetic fields (RF-EMF) can induce reactive oxygen species (ROS) release and/or changes in heat shock protein 70 (Hsp70) expression in human blood cells, using different exposure and co-exposure conditions. Human umbilical cord blood-derived monocytes and lymphocytes were used to examine ROS release after exposure to continuous wave or different GSM signals (GSM-DTX and GSM-Talk) at 2 W/kg for 30 or 45 min of continuous or intermittent (5 min ON/5 min OFF) exposure. The cells were exposed to incubator conditions, to sham, to RF-EMF, or to chemicals in parallel. Cell stimulation with the phorbol ester phorbol-12-myristate-13-acetate (PMA; 1 microM) was used as positive control for ROS release. To investigate the effects on Hsp70 expression, the human monocytes were exposed to the GSM-DTX signal at 2 W/kg for 45 min, or to heat treatment (42 degrees C) as positive control. ROS production and Hsp70 expression were determined by flow cytometric analysis. The data were compared to sham and/or to control values and the statistical analysis was performed by the Student's t-test (P<0.05). The PMA treatment induced a significant increase in ROS production in human monocytes and lymphocytes when the data were compared to sham or to incubator controls. After continuous or intermittent GSM-DTX signal exposure (2 W/kg), a significantly different ROS production was detected in human monocytes if the data were compared to sham. However, this significant difference appeared due to the lowered value of ROS release during sham exposure. In human lymphocytes, no differences could be detected if data were compared either to sham or to incubator control. The Hsp70 expression level after 0, 1, and 2 h post-exposure to GSM-DTX signal at 2 W/kg for 1 h did not show any differences compared to the incubator or to sham control.
Citation:
Radiat Environ Biophys 2006, 45 (1):55-62
Journal:
Radiation and environmental biophysics
Issue Date:
May-2006
URI:
http://hdl.handle.net/10146/64996
DOI:
10.1007/s00411-006-0038-3
PubMed ID:
16552570
Additional Links:
http://www.springerlink.com/content/h8647022q3268651/
Type:
Article
Language:
en
Description:
KEYWORDS CLASSIFICATION: analysis;Antigens,CD;Antigens,Differentiation,T-Lymphocyte;biosynthesis;blood;Biology;cytology;Dose-Response Relationship,Radiation;Electromagnetic Fields;Flow Cytometry;Germany;Heat-Shock Proteins;HSP70 Heat-Shock Proteins;Humans;Immunophenotyping;Lymphocytes;mechanisms of carcinogenesis;metabolism;Monocytes;pharmacology;physiology;Proteins;radiation effects;Radio Waves;Reactive Oxygen Species;Research;Tetradecanoylphorbol Acetate;Umbilical Cord;Umbilical Veins; A r i ΰ l ;biomarkers of exposure & effect: validation.
ISSN:
0301-634X
Appears in Collections:
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Full metadata record

DC FieldValue Language
dc.contributor.authorLantow, M.-
dc.contributor.authorLupke, M.-
dc.contributor.authorFrahm, J.-
dc.contributor.authorMattsson, M. O.-
dc.contributor.authorKuster, N.-
dc.contributor.authorSimko, M.-
dc.date.accessioned2009-04-15T12:05:12Z-
dc.date.available2009-04-15T12:05:12Z-
dc.date.issued2006-05-
dc.identifier.citationRadiat Environ Biophys 2006, 45 (1):55-62en
dc.identifier.issn0301-634X-
dc.identifier.pmid16552570-
dc.identifier.doi10.1007/s00411-006-0038-3-
dc.identifier.urihttp://hdl.handle.net/10146/64996-
dc.descriptionKEYWORDS CLASSIFICATION: analysis;Antigens,CD;Antigens,Differentiation,T-Lymphocyte;biosynthesis;blood;Biology;cytology;Dose-Response Relationship,Radiation;Electromagnetic Fields;Flow Cytometry;Germany;Heat-Shock Proteins;HSP70 Heat-Shock Proteins;Humans;Immunophenotyping;Lymphocytes;mechanisms of carcinogenesis;metabolism;Monocytes;pharmacology;physiology;Proteins;radiation effects;Radio Waves;Reactive Oxygen Species;Research;Tetradecanoylphorbol Acetate;Umbilical Cord;Umbilical Veins; A r i ΰ l ;biomarkers of exposure & effect: validation.en
dc.description.abstractThe aim of this study is to investigate if 1,800 MHz radiofrequency electromagnetic fields (RF-EMF) can induce reactive oxygen species (ROS) release and/or changes in heat shock protein 70 (Hsp70) expression in human blood cells, using different exposure and co-exposure conditions. Human umbilical cord blood-derived monocytes and lymphocytes were used to examine ROS release after exposure to continuous wave or different GSM signals (GSM-DTX and GSM-Talk) at 2 W/kg for 30 or 45 min of continuous or intermittent (5 min ON/5 min OFF) exposure. The cells were exposed to incubator conditions, to sham, to RF-EMF, or to chemicals in parallel. Cell stimulation with the phorbol ester phorbol-12-myristate-13-acetate (PMA; 1 microM) was used as positive control for ROS release. To investigate the effects on Hsp70 expression, the human monocytes were exposed to the GSM-DTX signal at 2 W/kg for 45 min, or to heat treatment (42 degrees C) as positive control. ROS production and Hsp70 expression were determined by flow cytometric analysis. The data were compared to sham and/or to control values and the statistical analysis was performed by the Student's t-test (P<0.05). The PMA treatment induced a significant increase in ROS production in human monocytes and lymphocytes when the data were compared to sham or to incubator controls. After continuous or intermittent GSM-DTX signal exposure (2 W/kg), a significantly different ROS production was detected in human monocytes if the data were compared to sham. However, this significant difference appeared due to the lowered value of ROS release during sham exposure. In human lymphocytes, no differences could be detected if data were compared either to sham or to incubator control. The Hsp70 expression level after 0, 1, and 2 h post-exposure to GSM-DTX signal at 2 W/kg for 1 h did not show any differences compared to the incubator or to sham control.en
dc.language.isoenen
dc.relation.urlhttp://www.springerlink.com/content/h8647022q3268651/en
dc.subject.meshAntigens, CD-
dc.subject.meshAntigens, Differentiation, T-Lymphocyte-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshElectromagnetic Fields-
dc.subject.meshFlow Cytometry-
dc.subject.meshHSP70 Heat-Shock Proteins-
dc.subject.meshHumans-
dc.subject.meshImmunophenotyping-
dc.subject.meshLymphocytes-
dc.subject.meshMonocytes-
dc.subject.meshRadio Waves-
dc.subject.meshReactive Oxygen Species-
dc.subject.meshTetradecanoylphorbol Acetate-
dc.subject.meshUmbilical Cord-
dc.subject.meshUmbilical Veins-
dc.titleROS release and Hsp70 expression after exposure to 1,800 MHz radiofrequency electromagnetic fields in primary human monocytes and lymphocytes.en
dc.typeArticleen
dc.identifier.journalRadiation and environmental biophysicsen

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