Immunohistochemical study of cell proliferation, Bcl-2, p53, and caspase-3 expression on preneoplastic changes induced by cadmium and zinc chloride in the ventral rat prostate.

2.50
Hdl Handle:
http://hdl.handle.net/10146/64475
Title:
Immunohistochemical study of cell proliferation, Bcl-2, p53, and caspase-3 expression on preneoplastic changes induced by cadmium and zinc chloride in the ventral rat prostate.
Authors:
Arriazu, Riánsares; Pozuelo, José M.; Henriques-Gil, Nuno; Perucho, Teresa; Martín, Rocío; Rodríguez, Rosario; Santamaría, Luis
Abstract:
This work was directed to evaluate immunoexpression of markers for apoptosis, resistance to apoptosis, and cell proliferation, as well as estimates of nuclear size in ventral prostate of rats treated with cadmium chloride and cadmium+zinc chloride because a possible protective effect of zinc has been postulated. The following variables were studied: volume fraction (VF) of Bcl-2 immunostaining, percentage of cells immunoreactive to proliferating cell nuclear antigen (LIPCNA) and p53 (LIp53), numerical density of caspase-3 immunoreactive cells (NV caspase-3), and estimates of volume-weighted mean nuclear volume (upsilonV). The LIPCNA and VF of Bcl-2 were significantly increased in the treated animals. The dysplasias (independent of their origin) showed a significant increase of the LIp53, NV caspase-3, and upsilonV in comparison with normal acini from treated and control animals. It can be concluded that cell proliferation is enhanced in long-term cadmium-exposed rats, and exposure to zinc combined with cadmium had no effect on any of the variables studied when comparing with normal acini. The increase of nuclear upsilonV could indicate a more aggressive behavior for pretumoral lesions.
Citation:
J. Histochem. Cytochem. 2006, 54 (9):981-990
Journal:
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Issue Date:
Sep-2006
URI:
http://hdl.handle.net/10146/64475
DOI:
10.1369/jhc.5A6733.2006
PubMed ID:
16585387
Additional Links:
http://www.jhc.org/cgi/content/full/54/9/981
Type:
Article
Language:
en
Description:
KEYWORDS CLASSIFICATION: Animals;Apoptosis;biosynthesis;Biology;chemically induced;Cadmium;Cadmium Chloride;Carcinogens;Caspase 3;Caspases;Cell Proliferation;Chlorides;Immunohistochemistry;metabolism;Male;mechanisms of carcinogenesis;pathology;pharmacology;Precancerous Conditions;Proliferating Cell Nuclear Antigen;Prostate;Prostatic Intraepithelial Neoplasia;Prostatic Neoplasms;Proteins;Proto-Oncogene Proteins;Proto-Oncogene Proteins c-bcl-2;Rats;Rats,Sprague-Dawley;Research;Spain;toxicity;Time Factors;Tumor Suppressor Protein p53;Zinc;Zinc Compounds.
ISSN:
0022-1554
Appears in Collections:
Articles with annotation

Full metadata record

DC FieldValue Language
dc.contributor.authorArriazu, Riánsares-
dc.contributor.authorPozuelo, José M.-
dc.contributor.authorHenriques-Gil, Nuno-
dc.contributor.authorPerucho, Teresa-
dc.contributor.authorMartín, Rocío-
dc.contributor.authorRodríguez, Rosario-
dc.contributor.authorSantamaría, Luis-
dc.date.accessioned2009-04-06T08:08:40Z-
dc.date.available2009-04-06T08:08:40Z-
dc.date.issued2006-09-
dc.identifier.citationJ. Histochem. Cytochem. 2006, 54 (9):981-990en
dc.identifier.issn0022-1554-
dc.identifier.pmid16585387-
dc.identifier.doi10.1369/jhc.5A6733.2006-
dc.identifier.urihttp://hdl.handle.net/10146/64475-
dc.descriptionKEYWORDS CLASSIFICATION: Animals;Apoptosis;biosynthesis;Biology;chemically induced;Cadmium;Cadmium Chloride;Carcinogens;Caspase 3;Caspases;Cell Proliferation;Chlorides;Immunohistochemistry;metabolism;Male;mechanisms of carcinogenesis;pathology;pharmacology;Precancerous Conditions;Proliferating Cell Nuclear Antigen;Prostate;Prostatic Intraepithelial Neoplasia;Prostatic Neoplasms;Proteins;Proto-Oncogene Proteins;Proto-Oncogene Proteins c-bcl-2;Rats;Rats,Sprague-Dawley;Research;Spain;toxicity;Time Factors;Tumor Suppressor Protein p53;Zinc;Zinc Compounds.en
dc.description.abstractThis work was directed to evaluate immunoexpression of markers for apoptosis, resistance to apoptosis, and cell proliferation, as well as estimates of nuclear size in ventral prostate of rats treated with cadmium chloride and cadmium+zinc chloride because a possible protective effect of zinc has been postulated. The following variables were studied: volume fraction (VF) of Bcl-2 immunostaining, percentage of cells immunoreactive to proliferating cell nuclear antigen (LIPCNA) and p53 (LIp53), numerical density of caspase-3 immunoreactive cells (NV caspase-3), and estimates of volume-weighted mean nuclear volume (upsilonV). The LIPCNA and VF of Bcl-2 were significantly increased in the treated animals. The dysplasias (independent of their origin) showed a significant increase of the LIp53, NV caspase-3, and upsilonV in comparison with normal acini from treated and control animals. It can be concluded that cell proliferation is enhanced in long-term cadmium-exposed rats, and exposure to zinc combined with cadmium had no effect on any of the variables studied when comparing with normal acini. The increase of nuclear upsilonV could indicate a more aggressive behavior for pretumoral lesions.en
dc.language.isoenen
dc.relation.urlhttp://www.jhc.org/cgi/content/full/54/9/981en
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshCadmium Chloride-
dc.subject.meshCarcinogens-
dc.subject.meshCaspase 3-
dc.subject.meshCaspases-
dc.subject.meshCell Proliferation-
dc.subject.meshChlorides-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMale-
dc.subject.meshPrecancerous Conditions-
dc.subject.meshProliferating Cell Nuclear Antigen-
dc.subject.meshProstate-
dc.subject.meshProstatic Intraepithelial Neoplasia-
dc.subject.meshProstatic Neoplasms-
dc.subject.meshProto-Oncogene Proteins c-bcl-2-
dc.subject.meshRats-
dc.subject.meshRats, Sprague-Dawley-
dc.subject.meshTime Factors-
dc.subject.meshTumor Suppressor Protein p53-
dc.subject.meshZinc Compounds-
dc.titleImmunohistochemical study of cell proliferation, Bcl-2, p53, and caspase-3 expression on preneoplastic changes induced by cadmium and zinc chloride in the ventral rat prostate.en
dc.typeArticleen
dc.identifier.journalThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Societyen
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