The mechanism of DNA damage induced by Roundup 360 PLUS, glyphosate and AMPA in human peripheral blood mononuclear cells - genotoxic risk assessement.

2.50
Hdl Handle:
http://hdl.handle.net/10146/618249
Title:
The mechanism of DNA damage induced by Roundup 360 PLUS, glyphosate and AMPA in human peripheral blood mononuclear cells - genotoxic risk assessement.
Authors:
Woźniak, Ewelina; Sicińska, Paulina; Michałowicz, Jaromir; Woźniak, Katarzyna; Reszka, Edyta; Huras, Bogumiła; Zakrzewski, Jerzy; Bukowska, Bożena
Abstract:
Glyphosate is the most heavily applied among pesticides in the world, and thus human exposure to this substance continues to increase. WHO changed classification of glyphosate to probably cancerogenic to humans, thus there is urgent need to assess in detail genotoxic mechanism of its action. We have assessed the effect of glyphosate, its formulation (Roundup 360 PLUS) and its main metabolite (aminomethylphosphonic acid, AMPA) in the concentration range from 1 to 1000 μM on DNA damage in human peripheral blood mononuclear cells (PBMCs). The cells were incubated for 24 h. The compounds studied and formulation induced DNA single and double strand-breaks and caused purines and pyrimidines oxidation. None of compounds examined was capable of creating adducts with DNA, while those substances increased ROS (including •OH) level in PBMCs. Roundup 360 PLUS caused damage to DNA even at 5 μM, while glyphosate and particularly AMPA induced DNA lesions from the concentration of 250 μM and 500 μM, respectively. DNA damage induced by glyphosate and its derivatives increased in order: AMPA, glyphosate, Roundup 360 PLUS. We may conclude that observed changes were not associated with direct interaction of xenobiotics studied with DNA, but the most probably they occurred through ROS-mediated effects.
Affiliation:
Nofer Institute of Occupational Medicine
Citation:
Food Chem. Toxicol. 2018, 120:510-522
Journal:
Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association
Issue Date:
Oct-2018
URI:
http://hdl.handle.net/10146/618249
DOI:
10.1016/j.fct.2018.07.035
PubMed ID:
30055318
Additional Links:
https://www.sciencedirect.com/science/article/pii/S0278691518304800?via%3Dihub
Type:
Article
Language:
en
ISSN:
1873-6351
Sponsors:
The investigation was supported by a statutory research admitted for Department of Biophysics of Environmental Pollution, University of Lodz (number B16/17 000000191.01).
Appears in Collections:
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Full metadata record

DC FieldValue Language
dc.contributor.authorWoźniak, Ewelinaen
dc.contributor.authorSicińska, Paulinaen
dc.contributor.authorMichałowicz, Jaromiren
dc.contributor.authorWoźniak, Katarzynaen
dc.contributor.authorReszka, Edytaen
dc.contributor.authorHuras, Bogumiłaen
dc.contributor.authorZakrzewski, Jerzyen
dc.contributor.authorBukowska, Bożenaen
dc.date.accessioned2018-12-05T09:49:09Z-
dc.date.available2018-12-05T09:49:09Z-
dc.date.issued2018-10-
dc.identifier.citationFood Chem. Toxicol. 2018, 120:510-522en
dc.identifier.issn1873-6351-
dc.identifier.pmid30055318-
dc.identifier.doi10.1016/j.fct.2018.07.035-
dc.identifier.urihttp://hdl.handle.net/10146/618249-
dc.description.abstractGlyphosate is the most heavily applied among pesticides in the world, and thus human exposure to this substance continues to increase. WHO changed classification of glyphosate to probably cancerogenic to humans, thus there is urgent need to assess in detail genotoxic mechanism of its action. We have assessed the effect of glyphosate, its formulation (Roundup 360 PLUS) and its main metabolite (aminomethylphosphonic acid, AMPA) in the concentration range from 1 to 1000 μM on DNA damage in human peripheral blood mononuclear cells (PBMCs). The cells were incubated for 24 h. The compounds studied and formulation induced DNA single and double strand-breaks and caused purines and pyrimidines oxidation. None of compounds examined was capable of creating adducts with DNA, while those substances increased ROS (including •OH) level in PBMCs. Roundup 360 PLUS caused damage to DNA even at 5 μM, while glyphosate and particularly AMPA induced DNA lesions from the concentration of 250 μM and 500 μM, respectively. DNA damage induced by glyphosate and its derivatives increased in order: AMPA, glyphosate, Roundup 360 PLUS. We may conclude that observed changes were not associated with direct interaction of xenobiotics studied with DNA, but the most probably they occurred through ROS-mediated effects.en
dc.description.sponsorshipThe investigation was supported by a statutory research admitted for Department of Biophysics of Environmental Pollution, University of Lodz (number B16/17 000000191.01).en
dc.language.isoenen
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0278691518304800?via%3Dihuben
dc.rightsArchived with thanks to Food and chemical toxicology : an international journal published for the British Industrial Biological Research Associationen
dc.subjectDNA adductsen
dc.subjectDNA basesen
dc.subjectGlyphosateen
dc.subjectPBMCsen
dc.subjectreactive oxygen speciesen
dc.subjectRoundup 360 PLUSen
dc.titleThe mechanism of DNA damage induced by Roundup 360 PLUS, glyphosate and AMPA in human peripheral blood mononuclear cells - genotoxic risk assessement.en
dc.typeArticleen
dc.contributor.departmentNofer Institute of Occupational Medicineen
dc.identifier.journalFood and Chemical Toxicology : an international journal published for the British Industrial Biological Research Associationen
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