The ESR1 and GPX1 gene expression level in human malignant and non-malignant breast tissues.

2.50
Hdl Handle:
http://hdl.handle.net/10146/618206
Title:
The ESR1 and GPX1 gene expression level in human malignant and non-malignant breast tissues.
Authors:
Król, Magdalena B; Galicki, Michał; Grešner, Peter ( 0000-0002-7008-396X ) ; Wieczorek, Edyta ( 0000-0002-4390-2042 ) ; Jabłońska, Ewa ( 0000-0002-3946-3964 ) ; Reszka, Edyta ( 0000-0003-2153-4864 ) ; Morawiec, Zbigniew; Wąsowicz, Wojciech ( 0000-0002-2991-9040 ) ; Gromadzińska, Jolanta
Abstract:
The aim of this study was to establish whether the gene expression of estrogen receptor alpha (encoded by ESR1) correlates with the expression of glutathione peroxidase 1 (encoded by GPX1) in the tumor and adjacent tumor-free breast tissue, and whether this correlation is affected by breast cancer. Such relationships may give further insights into breast cancer pathology with respect to the status of estrogen receptor.; We used the quantitative real-time PCR technique to analyze differences in the expression levels of the ESR1 and GPX1 genes in paired malignant and non-malignant tissues from breast cancer patients.; ESR1 and GPX1 expression levels were found to be significantly down-regulated by 14.7% and 7.4% (respectively) in the tumorous breast tissue when compared to the non-malignant one. Down-regulation of these genes was independent of the tumor histopathology classification and clinicopathological factors, while the ESR1 mRNA level was reduced with increasing tumor grade (G1: 103% vs. G2: 85.8% vs. G3: 84.5%; p<0.05). In the non-malignant and malignant breast tissues, the expression levels of ESR1 and GPX1 were significantly correlated with each other (Rs=0.450 and Rs=0.360; respectively).; Our data suggest that down-regulation of ESR1 and GPX1 was independent of clinicopathological factors. Down-regulation of ESR1 gene expression was enhanced by the development of the disease. Moreover, GPX1 and ESR1 gene expression was interdependent in the malignant breast tissue and further work is needed to determine the mechanism underlying this relationship.
Affiliation:
Nofer Institute of Occupational Medicine, Lodz, Poland
Citation:
Acta Biochim. Pol.2018 Vol. 65
Journal:
Acta Biochimica Polonica
Issue Date:
15-Mar-2018
URI:
http://hdl.handle.net/10146/618206
DOI:
10.18388/abp.2016_1425
PubMed ID:
29543921
Additional Links:
http://www.actabp.pl/pdf/Preprint/2016_1425.pdf
Type:
Article
Language:
en
ISSN:
1734-154X
Sponsors:
This study was funded by the Polish Ministry of Science and Higher Education as the internal grant of the Nofer Institute of Occupational Medicine (grant number: IMP1.12/2012-2014)
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorKról, Magdalena Ben
dc.contributor.authorGalicki, Michałen
dc.contributor.authorGrešner, Peteren
dc.contributor.authorWieczorek, Edytaen
dc.contributor.authorJabłońska, Ewaen
dc.contributor.authorReszka, Edytaen
dc.contributor.authorMorawiec, Zbigniewen
dc.contributor.authorWąsowicz, Wojciechen
dc.contributor.authorGromadzińska, Jolantaen
dc.date.accessioned2018-03-20T12:16:41Z-
dc.date.available2018-03-20T12:16:41Z-
dc.date.issued2018-03-15-
dc.identifier.citationActa Biochim. Pol.2018 Vol. 65en
dc.identifier.issn1734-154X-
dc.identifier.pmid29543921-
dc.identifier.doi10.18388/abp.2016_1425-
dc.identifier.urihttp://hdl.handle.net/10146/618206-
dc.description.abstractThe aim of this study was to establish whether the gene expression of estrogen receptor alpha (encoded by ESR1) correlates with the expression of glutathione peroxidase 1 (encoded by GPX1) in the tumor and adjacent tumor-free breast tissue, and whether this correlation is affected by breast cancer. Such relationships may give further insights into breast cancer pathology with respect to the status of estrogen receptor.en
dc.description.abstractWe used the quantitative real-time PCR technique to analyze differences in the expression levels of the ESR1 and GPX1 genes in paired malignant and non-malignant tissues from breast cancer patients.en
dc.description.abstractESR1 and GPX1 expression levels were found to be significantly down-regulated by 14.7% and 7.4% (respectively) in the tumorous breast tissue when compared to the non-malignant one. Down-regulation of these genes was independent of the tumor histopathology classification and clinicopathological factors, while the ESR1 mRNA level was reduced with increasing tumor grade (G1: 103% vs. G2: 85.8% vs. G3: 84.5%; p<0.05). In the non-malignant and malignant breast tissues, the expression levels of ESR1 and GPX1 were significantly correlated with each other (Rs=0.450 and Rs=0.360; respectively).en
dc.description.abstractOur data suggest that down-regulation of ESR1 and GPX1 was independent of clinicopathological factors. Down-regulation of ESR1 gene expression was enhanced by the development of the disease. Moreover, GPX1 and ESR1 gene expression was interdependent in the malignant breast tissue and further work is needed to determine the mechanism underlying this relationship.en
dc.description.sponsorshipThis study was funded by the Polish Ministry of Science and Higher Education as the internal grant of the Nofer Institute of Occupational Medicine (grant number: IMP1.12/2012-2014)en
dc.language.isoenen
dc.relation.urlhttp://www.actabp.pl/pdf/Preprint/2016_1425.pdfen
dc.rightsArchived with thanks to Acta biochimica Polonicaen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectestrogen receptoren
dc.subjectantioxidant enzymesen
dc.subjectgene expressionen
dc.subjectbreast cancer tissueen
dc.titleThe ESR1 and GPX1 gene expression level in human malignant and non-malignant breast tissues.en
dc.typeArticleen
dc.contributor.departmentNofer Institute of Occupational Medicine, Lodz, Polanden
dc.identifier.journalActa Biochimica Polonicaen

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