Rotating night work, lifestyle factors, obesity and promoter methylation in BRCA1 and BRCA2 genes among nurses and midwives.

2.50
Hdl Handle:
http://hdl.handle.net/10146/618141
Title:
Rotating night work, lifestyle factors, obesity and promoter methylation in BRCA1 and BRCA2 genes among nurses and midwives.
Authors:
Peplonska, Beata; Bukowska, Agnieszka ( 0000-0001-8167-4215 ) ; Wieczorek, Edyta; Przybek, Monika; Zienolddiny, Shanbeh; Reszka, Edyta ( 0000-0003-2153-4864 )
Abstract:
Some recent evidence suggests that environmental and lifestyle factors may modify DNA methylation. We hypothesized that rotating night work and several modifiable factors may be associated with the methylation of the promoter regions within two tumor suppressor and DNA repair genes: BRCA1 and BRCA2. The methylation status of BRCA1 and BRCA2 was determined via qMSP reactions using DNA samples derived from blood leucocytes of 347 nurses and midwives working rotating nights and 363 working during the days. The subjects were classified into unmethylated vs methylated BRCA1 and BRCA2 when the methylation index was 0% or >0%, respectively. The adjusted odds ratios with 95% confidence intervals were calculated for night work status, smoking, obesity, physical activity and alcohol drinking. Current night shift work or night work history was not associated with methylation status of the promoter sites within BRCA1 and BRCA2 genes. We observed weak associations between smoking and the methylation status of BRCA1 with OR = 1.50 (95%CI: 0.98-2.29) for current smoking, OR = 1.83, 95CI: 1.08-3.13 for smoking longer than 31 years, and 0.1>p>0.05 for trends for the number of cigarettes per day, smoking duration and packyears. In conclusion, no links between night shift work and methylation of the promoter region within the BRCA1, and BRCA2 genes were observed in this exploratory analysis. The findings of our study weakly support the hypothesis that smoking may contribute to epigenetic events.
Affiliation:
Nofer Institute of Occupational Medicine, Łódź, Poland
Citation:
PLoS ONE 2017, 12 (6):e0178792
Journal:
PloS ONE
Issue Date:
2017
URI:
http://hdl.handle.net/10146/618141
DOI:
10.1371/journal.pone.0178792
PubMed ID:
28594926
Additional Links:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0178792
Type:
Article
Language:
en
ISSN:
1932-6203
Sponsors:
Funding: This project was supported by the Norway Grants, under the Polish-Norwegian Research Programme (Grant no. PNRF ± 243 ± AI ± 1/07 and Pol-Nor/196940/22/2013-clockshift) (BP). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorPeplonska, Beataen
dc.contributor.authorBukowska, Agnieszkaen
dc.contributor.authorWieczorek, Edytaen
dc.contributor.authorPrzybek, Monikaen
dc.contributor.authorZienolddiny, Shanbehen
dc.contributor.authorReszka, Edytaen
dc.date.accessioned2017-08-24T11:15:47Z-
dc.date.available2017-08-24T11:15:47Z-
dc.date.issued2017-
dc.identifier.citationPLoS ONE 2017, 12 (6):e0178792en
dc.identifier.issn1932-6203-
dc.identifier.pmid28594926-
dc.identifier.doi10.1371/journal.pone.0178792-
dc.identifier.urihttp://hdl.handle.net/10146/618141-
dc.description.abstractSome recent evidence suggests that environmental and lifestyle factors may modify DNA methylation. We hypothesized that rotating night work and several modifiable factors may be associated with the methylation of the promoter regions within two tumor suppressor and DNA repair genes: BRCA1 and BRCA2. The methylation status of BRCA1 and BRCA2 was determined via qMSP reactions using DNA samples derived from blood leucocytes of 347 nurses and midwives working rotating nights and 363 working during the days. The subjects were classified into unmethylated vs methylated BRCA1 and BRCA2 when the methylation index was 0% or >0%, respectively. The adjusted odds ratios with 95% confidence intervals were calculated for night work status, smoking, obesity, physical activity and alcohol drinking. Current night shift work or night work history was not associated with methylation status of the promoter sites within BRCA1 and BRCA2 genes. We observed weak associations between smoking and the methylation status of BRCA1 with OR = 1.50 (95%CI: 0.98-2.29) for current smoking, OR = 1.83, 95CI: 1.08-3.13 for smoking longer than 31 years, and 0.1>p>0.05 for trends for the number of cigarettes per day, smoking duration and packyears. In conclusion, no links between night shift work and methylation of the promoter region within the BRCA1, and BRCA2 genes were observed in this exploratory analysis. The findings of our study weakly support the hypothesis that smoking may contribute to epigenetic events.en
dc.description.sponsorshipFunding: This project was supported by the Norway Grants, under the Polish-Norwegian Research Programme (Grant no. PNRF ± 243 ± AI ± 1/07 and Pol-Nor/196940/22/2013-clockshift) (BP). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.language.isoenen
dc.relation.hasparthttp://ecnis.openrepository.com/ecnis/handle/10146/618115en
dc.relation.urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0178792en
dc.rightsArchived with thanks to PloS oneen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectsmoking habitsen
dc.subjectbreast canceren
dc.subjectAlcohol consumptionen
dc.subjectnursesen
dc.titleRotating night work, lifestyle factors, obesity and promoter methylation in BRCA1 and BRCA2 genes among nurses and midwives.en
dc.typeArticleen
dc.contributor.departmentNofer Institute of Occupational Medicine, Łódź, Polanden
dc.identifier.journalPloS ONEen

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