Developmental toxicity of N-methylaniline following prenatal oral administration in rats

2.50
Hdl Handle:
http://hdl.handle.net/10146/601580
Title:
Developmental toxicity of N-methylaniline following prenatal oral administration in rats
Authors:
Sitarek, Krystyna ( 0000-0002-2624-0956 ) ; Gromadzinska, Jolanta; Stetkiewicz, Jan; Lutz, Piotr; Krol, Magdalena ( 0000-0002-9564-3257 ) ; Domeradzka-Gajda, Katarzyna; Wasowicz, Wojciech ( 0000-0002-2991-9040 )
Abstract:
Objectives: The objective of the study was to assess prenatal toxicity of N-methylaniline (NMA) administered by gavage to pregnant female rats. Material and Methods: Pregnant female rats were administered N-methylaniline in corn oil by gavage at daily doses of 0.8 mg/kg of body weight (b.w.), 4 mg/kg b.w., 20 mg/kg b.w. and 100 mg/kg b.w. from implantation (the 5th day post mating) to the day prior to the scheduled caesarean section (the 20th day of pregnancy). General behavior, body weight, food and water consumption, hematological, biochemical analyses and pathomorphological changes of the dams were recorded. Results: All the females survived until the end of the study. The test substance was toxic to pregnant females, even at the lowest of the used doses, i.e., 0.8 mg/kg b.w./day. Lower weight gain during pregnancy and significantly higher NMA-dose-dependent absolute weight of the organs were noted in the exposed females. The females from the groups exposed at doses of 20 mg/kg b.w./day and 100 mg/kg b.w./day developed anemia and showed higher concentrations of free thyroxine (FT3) and free triiodothyronine (FT4) thyroid hormones. Total protein concentration exhibited an increase in all the exposed groups of females. In the prenatal toxicity study, administration of N-methylaniline throughout the embryonic and fetal periods produced embryotoxic effects at doses ranging 4–100 mg/kg b.w./day. Conclusions: Considering the data obtained in this study, it is reasonable to assume that N-methylaniline administered orally to pregnant rats is toxic for mothers even at a low dose of 0.8 mg/kg b.w./day. However, this dose was not associated with any significant effects to their offspring. This prenatal exposure level may be considered as no-observed-adverse-effect level (NOAEL) for the progeny and a dose of 4 mg/kg b.w./day as the lowest-observed-adverse-effect level (LOAEL) for the progeny.
Affiliation:
Nofer Institute of Occupational Medicine, Lodz, Poland
Citation:
Int J Occup Med Environ Health 2016;29(3)
Journal:
International Journal of Occupational Medicine and Environmental Health
Issue Date:
2016
URI:
http://hdl.handle.net/10146/601580
DOI:
10.13075/ijomeh.1896.00571
Additional Links:
http://www.journalssystem.com/ijomeh/DEVELOPMENTAL-TOXICITY-OF-N-METHYLANILINE-FOLLOWING-PRENATALLY-ORAL-ADMINISTRATION-OF-RATS,58669,0,2.html; http://ijomeh.eu/Developmental-toxicity-of-N-methylaniline-following-prenatal-oral-administration-in-rats,58669,0,2.html
Type:
Article
Language:
en_US
ISSN:
1232-1087
Sponsors:
“Assessment of prenatal toxicity of rats exposed intragastrically to N-methylaniline,” project IMP No. 1.9.
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorSitarek, Krystynaen
dc.contributor.authorGromadzinska, Jolantaen
dc.contributor.authorStetkiewicz, Janen
dc.contributor.authorLutz, Piotren
dc.contributor.authorKrol, Magdalenaen
dc.contributor.authorDomeradzka-Gajda, Katarzynaen
dc.contributor.authorWasowicz, Wojciechen
dc.date.accessioned2016-03-17T13:16:00Zen
dc.date.available2016-03-17T13:16:00Zen
dc.date.issued2016en
dc.identifier.citationInt J Occup Med Environ Health 2016;29(3)en
dc.identifier.issn1232-1087en
dc.identifier.doi10.13075/ijomeh.1896.00571en
dc.identifier.urihttp://hdl.handle.net/10146/601580en
dc.description.abstractObjectives: The objective of the study was to assess prenatal toxicity of N-methylaniline (NMA) administered by gavage to pregnant female rats. Material and Methods: Pregnant female rats were administered N-methylaniline in corn oil by gavage at daily doses of 0.8 mg/kg of body weight (b.w.), 4 mg/kg b.w., 20 mg/kg b.w. and 100 mg/kg b.w. from implantation (the 5th day post mating) to the day prior to the scheduled caesarean section (the 20th day of pregnancy). General behavior, body weight, food and water consumption, hematological, biochemical analyses and pathomorphological changes of the dams were recorded. Results: All the females survived until the end of the study. The test substance was toxic to pregnant females, even at the lowest of the used doses, i.e., 0.8 mg/kg b.w./day. Lower weight gain during pregnancy and significantly higher NMA-dose-dependent absolute weight of the organs were noted in the exposed females. The females from the groups exposed at doses of 20 mg/kg b.w./day and 100 mg/kg b.w./day developed anemia and showed higher concentrations of free thyroxine (FT3) and free triiodothyronine (FT4) thyroid hormones. Total protein concentration exhibited an increase in all the exposed groups of females. In the prenatal toxicity study, administration of N-methylaniline throughout the embryonic and fetal periods produced embryotoxic effects at doses ranging 4–100 mg/kg b.w./day. Conclusions: Considering the data obtained in this study, it is reasonable to assume that N-methylaniline administered orally to pregnant rats is toxic for mothers even at a low dose of 0.8 mg/kg b.w./day. However, this dose was not associated with any significant effects to their offspring. This prenatal exposure level may be considered as no-observed-adverse-effect level (NOAEL) for the progeny and a dose of 4 mg/kg b.w./day as the lowest-observed-adverse-effect level (LOAEL) for the progeny.en
dc.description.sponsorship“Assessment of prenatal toxicity of rats exposed intragastrically to N-methylaniline,” project IMP No. 1.9.en
dc.language.isoen_USen
dc.relation.urlhttp://www.journalssystem.com/ijomeh/DEVELOPMENTAL-TOXICITY-OF-N-METHYLANILINE-FOLLOWING-PRENATALLY-ORAL-ADMINISTRATION-OF-RATS,58669,0,2.htmlen
dc.relation.urlhttp://ijomeh.eu/Developmental-toxicity-of-N-methylaniline-following-prenatal-oral-administration-in-rats,58669,0,2.htmlen
dc.rightsArchived with thanks to International Journal of Occupational Medicine and Environmental Healthen
dc.subjectRaten
dc.subjectN-methylanilineen
dc.subjectNMAen
dc.subjectDevelopmental toxicityen
dc.subjectmetHben
dc.subjectToxicityen
dc.titleDevelopmental toxicity of N-methylaniline following prenatal oral administration in ratsen_US
dc.typeArticleen
dc.contributor.departmentNofer Institute of Occupational Medicine, Lodz, Polanden
dc.identifier.journalInternational Journal of Occupational Medicine and Environmental Healthen
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