Binding of galectin-1 (gal-1) to the Thomsen-Friedenreich (TF) antigen on trophoblast cells and inhibition of proliferation of trophoblast tumor cells in vitro by gal-1 or an anti-TF antibody.

2.50
Hdl Handle:
http://hdl.handle.net/10146/57793
Title:
Binding of galectin-1 (gal-1) to the Thomsen-Friedenreich (TF) antigen on trophoblast cells and inhibition of proliferation of trophoblast tumor cells in vitro by gal-1 or an anti-TF antibody.
Authors:
Jeschke, Udo; Karsten, Uwe; Wiest, Irmi; Schulze, Sandra; Kuhn, Christina; Friese, Klaus; Walzel, Hermann
Abstract:
Galectin-1 (gal-1), a member of the mammalian beta-galactoside-binding proteins, recognizes preferentially Galbeta1-4GlcNAc sequences of several cell surface oligosaccharides. We demonstrate histochemically that the lectin recognizes appropriate glycotopes on the syncytiotrophoblast and extravillous trophoblast layer from second trimester human placenta and on BeWo chorion carcinoma cells. Gal-1 binding to BeWo cells was diminished by the Thomsen-Friedreich (TF)-disaccharide (Galbeta1-3GalNAc-) conjugated to polyacrylamide (TF-PAA). Gal-1 also inhibited BeWo cell proliferation in a concentration-dependent manner. Similar antiproliferative effects were also observed with an anti-TF monoclonal antibody (mAb, A78-G/A7). Therefore, we conclude that ligation of Galbeta1-4GlcNAc and Galbeta1-3GalNAc epitopes on BeWo cells may have regulatory effects on cell proliferation.
Citation:
Histochem. Cell Biol. 2006, 126 (4):437-444
Journal:
Histochemistry and cell biology
Issue Date:
Oct-2006
URI:
http://hdl.handle.net/10146/57793
DOI:
10.1007/s00418-006-0178-1
PubMed ID:
16607538
Additional Links:
http://www.springerlink.com/content/863153823336r6v2/
Type:
Article
Language:
en
Description:
KEYWORDS CLASSIFICATION: Antibodies;Antigens,Tumor-Associated,Carbohydrate;Cell Line,Tumor;Cell Proliferation;drug effects;Female;Galectin 1;Germany;Glycophorin;Humans;immunology;Immunoglobulin M;metabolism;mechanisms of carcinogenesis;pharmacology;Research;Trophoblastic Neoplasms;Trophoblasts.
ISSN:
0948-6143
Appears in Collections:
Articles with annotation

Full metadata record

DC FieldValue Language
dc.contributor.authorJeschke, Udo-
dc.contributor.authorKarsten, Uwe-
dc.contributor.authorWiest, Irmi-
dc.contributor.authorSchulze, Sandra-
dc.contributor.authorKuhn, Christina-
dc.contributor.authorFriese, Klaus-
dc.contributor.authorWalzel, Hermann-
dc.date.accessioned2009-03-30T07:46:10Z-
dc.date.available2009-03-30T07:46:10Z-
dc.date.issued2006-10-
dc.identifier.citationHistochem. Cell Biol. 2006, 126 (4):437-444en
dc.identifier.issn0948-6143-
dc.identifier.pmid16607538-
dc.identifier.doi10.1007/s00418-006-0178-1-
dc.identifier.urihttp://hdl.handle.net/10146/57793-
dc.descriptionKEYWORDS CLASSIFICATION: Antibodies;Antigens,Tumor-Associated,Carbohydrate;Cell Line,Tumor;Cell Proliferation;drug effects;Female;Galectin 1;Germany;Glycophorin;Humans;immunology;Immunoglobulin M;metabolism;mechanisms of carcinogenesis;pharmacology;Research;Trophoblastic Neoplasms;Trophoblasts.en
dc.description.abstractGalectin-1 (gal-1), a member of the mammalian beta-galactoside-binding proteins, recognizes preferentially Galbeta1-4GlcNAc sequences of several cell surface oligosaccharides. We demonstrate histochemically that the lectin recognizes appropriate glycotopes on the syncytiotrophoblast and extravillous trophoblast layer from second trimester human placenta and on BeWo chorion carcinoma cells. Gal-1 binding to BeWo cells was diminished by the Thomsen-Friedreich (TF)-disaccharide (Galbeta1-3GalNAc-) conjugated to polyacrylamide (TF-PAA). Gal-1 also inhibited BeWo cell proliferation in a concentration-dependent manner. Similar antiproliferative effects were also observed with an anti-TF monoclonal antibody (mAb, A78-G/A7). Therefore, we conclude that ligation of Galbeta1-4GlcNAc and Galbeta1-3GalNAc epitopes on BeWo cells may have regulatory effects on cell proliferation.en
dc.language.isoenen
dc.relation.urlhttp://www.springerlink.com/content/863153823336r6v2/en
dc.subjectGalectin-1en
dc.subjectThomsen–Friedenreichen
dc.subjectImmunocytochemistryen
dc.subjectPlacentaen
dc.subjectBeWo cellsen
dc.subject.meshAntibodies-
dc.subject.meshAntigens, Tumor-Associated, Carbohydrate-
dc.subject.meshCell Line, Tumor-
dc.subject.meshCell Proliferation-
dc.subject.meshFemale-
dc.subject.meshGalectin 1-
dc.subject.meshGlycophorin-
dc.subject.meshHumans-
dc.subject.meshImmunoglobulin M-
dc.subject.meshTrophoblastic Neoplasms-
dc.subject.meshTrophoblasts-
dc.titleBinding of galectin-1 (gal-1) to the Thomsen-Friedenreich (TF) antigen on trophoblast cells and inhibition of proliferation of trophoblast tumor cells in vitro by gal-1 or an anti-TF antibody.en
dc.typeArticleen
dc.identifier.journalHistochemistry and cell biologyen

Related articles on PubMed

All Items in ECNIS-NIOM are protected by copyright, with all rights reserved, unless otherwise indicated.