Cancer chemopreventive and anti-inflammatory activities of chemically modified guar gum.

2.50
Hdl Handle:
http://hdl.handle.net/10146/57553
Title:
Cancer chemopreventive and anti-inflammatory activities of chemically modified guar gum.
Authors:
Gamal-Eldeen, Amira M.; Amer, Hassan; Helmy, Wafaa A.
Abstract:
Guar gum (G) is a simple characterized branched polysaccharide, which is frequently used in food industries. We prepared the gum C-glycosylated derivative (GG), and its sulphated derivative (SGG), aiming to characterize their cancer chemopreventive, and anti-inflammatory properties. Estimation of cancer chemopreventive activity, specifically anti-initiation, including the modulation of carcinogen metabolism and the antioxidant capacity, revealed that GG was a potent anti-initiator, where it inhibited not only the carcinogen activator enzyme, cytochrome P450 1A (CYP1A), but also induced the carcinogen detoxification enzymes glutathione-S-transferases (GSTs), while SGG inhibited both CYP1A and GSTs. SGG was an effective radical scavenger than GG against hydroxyl, peroxyl, and superoxide anion radicals. GG and SGG were found to modulate the macrophage functions into an anti-inflammatory pattern. Thus, both enhanced the macrophage proliferation and phagocytosis of fluorescein isothiocyanate (FITC)-zymosan; however, they also inhibited strongly the nitric oxide generation and tumor necrosis factor-alpha secretion in lipopolysaccharide (LPS)-stimulated RAW macrophage 264.7. Unexpectedly, both GG and SGG dramatically inhibited the binding affinity of FITC-LPS to RAW 264.7, as indicated by flow cytometry analysis. GG and SGG exhibited a significant anti-proliferative activity against human hepatocellular carcinoma cells (Hep G2), and only SGG was specifically cytotoxic for human breast carcinoma cells (MCF-7), but neither was significantly cytotoxic for human lymphoblastic leukemia cells (1301). SGG led to a major disturbance in cell cycle phases of Hep G2 cells as indicated by concomitant arrest in S- and G2/M-phases, a disturbance that was associated with an induced cell death as a result of necrosis, but not apoptosis in both GG- and SGG-treated cells. Taken together, the modified gums could be used as an alternative of G in health food industries to provide cancer prevention in risk populations.
Citation:
Chem. Biol. Interact. 2006, 161 (3):229-240
Journal:
Chemico-biological Interactions
Issue Date:
10-Jul-2006
URI:
http://hdl.handle.net/10146/57553
DOI:
10.1016/j.cbi.2006.03.010
PubMed ID:
16756967
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T56-4JN72CP-1&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=7f5ed225f126c234aaca7338330de6e3
Type:
Article
Language:
en
Description:
Dietary modulation of carcinogenesis-related pathways. Dietary item or component studied: gum C-glycosylated derivative (GG) and its sulphated derivative (SGG)Pathways studied: Anti-initiating activity [cytochrome P450 1A (CYP1A), glutathione-S-transferases (GSTs)]; antioxidant activity [reactive oxygen and nitrogen species (ROS and RNS)]; anti-promoting activity and modulation of macrophage function; Cell cycle analysis phases of HepG2 cells; analysis of apoptosis and necrosis. Study type: human lymphoblastic leukemia cells; human hepatocarcinoma (HepG2) cells; human breast carcinoma (MCF-7) cells; RAW murine macrophage (RAW264.7) cells Impact on pathway (including dose-response): Estimation of cancer chemopreventive activity, specifically anti-initiation, including the modulation of carcinogen metabolism and the antioxidant capacity, revealed that GG was a potent anti-initiator, where it inhibited not only the carcinogen activator enzyme, cytochrome P450 1A (CYP1A), but also induced the carcinogen detoxification enzymes glutathione-S-transferases (GSTs), while SGG inhibited both CYP1A and GSTs. SGG was an effective radical scavenger than GG against hydroxyl, peroxyl, and superoxide anion radicals. GG and SGG were found to modulate the macrophage functions into an anti-inflammatory pattern. Thus, both enhanced the macrophage proliferation and phagocytosis of fluorescein isothiocyanate (FITC)-zymosan; however, they also inhibited strongly the nitric oxide generation and tumor necrosis factor-alpha secretion in lipopolysaccharide (LPS)-stimulated RAW macrophage 264.7. Unexpectedly, both GG and SGG dramatically inhibited the binding affinity of FITC-LPS to RAW 264.7, as indicated by flow cytometry analysis. GG and SGG exhibited a significant anti-proliferative activity against human hepatocellular carcinoma cells (HepG2), and only SGG was specifically cytotoxic for human breast carcinoma cells (MCF-7), but neither was significantly cytotoxic for human lymphoblastic leukemia cells (1301). SGG led to a major disturbance in cell cycle phases of Hep G2 cells as indicated by concomitant arrest in S- and G2/M-phases, a disturbance that was associated with an induced cell death as a result of necrosis, but not apoptosis in both GG- and SGG-treated cells. KEYWORDS - CLASIFFICATION: analysis;Animals;Anti-Inflammatory Agents;Antineoplastic Agents;Antioxidants;Biology;Biotechnology;Cell Line;Cell Proliferation;chemistry;dietary modulation of carcinogenesis-related pathways;drug effects;Egypt;Galactans;Humans;Macrophages;Mannans;mechanisms of carcinogenesis;metabolism;Mice;Neoplasms;pathology;pharmacology;Plant Gums;prevention & control;Research;secretion;
ISSN:
0009-2797
Appears in Collections:
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Full metadata record

DC FieldValue Language
dc.contributor.authorGamal-Eldeen, Amira M.-
dc.contributor.authorAmer, Hassan-
dc.contributor.authorHelmy, Wafaa A.-
dc.date.accessioned2009-03-27T11:21:23Z-
dc.date.available2009-03-27T11:21:23Z-
dc.date.issued2006-07-10-
dc.identifier.citationChem. Biol. Interact. 2006, 161 (3):229-240en
dc.identifier.issn0009-2797-
dc.identifier.pmid16756967-
dc.identifier.doi10.1016/j.cbi.2006.03.010-
dc.identifier.urihttp://hdl.handle.net/10146/57553-
dc.descriptionDietary modulation of carcinogenesis-related pathways. Dietary item or component studied: gum C-glycosylated derivative (GG) and its sulphated derivative (SGG)Pathways studied: Anti-initiating activity [cytochrome P450 1A (CYP1A), glutathione-S-transferases (GSTs)]; antioxidant activity [reactive oxygen and nitrogen species (ROS and RNS)]; anti-promoting activity and modulation of macrophage function; Cell cycle analysis phases of HepG2 cells; analysis of apoptosis and necrosis. Study type: human lymphoblastic leukemia cells; human hepatocarcinoma (HepG2) cells; human breast carcinoma (MCF-7) cells; RAW murine macrophage (RAW264.7) cells Impact on pathway (including dose-response): Estimation of cancer chemopreventive activity, specifically anti-initiation, including the modulation of carcinogen metabolism and the antioxidant capacity, revealed that GG was a potent anti-initiator, where it inhibited not only the carcinogen activator enzyme, cytochrome P450 1A (CYP1A), but also induced the carcinogen detoxification enzymes glutathione-S-transferases (GSTs), while SGG inhibited both CYP1A and GSTs. SGG was an effective radical scavenger than GG against hydroxyl, peroxyl, and superoxide anion radicals. GG and SGG were found to modulate the macrophage functions into an anti-inflammatory pattern. Thus, both enhanced the macrophage proliferation and phagocytosis of fluorescein isothiocyanate (FITC)-zymosan; however, they also inhibited strongly the nitric oxide generation and tumor necrosis factor-alpha secretion in lipopolysaccharide (LPS)-stimulated RAW macrophage 264.7. Unexpectedly, both GG and SGG dramatically inhibited the binding affinity of FITC-LPS to RAW 264.7, as indicated by flow cytometry analysis. GG and SGG exhibited a significant anti-proliferative activity against human hepatocellular carcinoma cells (HepG2), and only SGG was specifically cytotoxic for human breast carcinoma cells (MCF-7), but neither was significantly cytotoxic for human lymphoblastic leukemia cells (1301). SGG led to a major disturbance in cell cycle phases of Hep G2 cells as indicated by concomitant arrest in S- and G2/M-phases, a disturbance that was associated with an induced cell death as a result of necrosis, but not apoptosis in both GG- and SGG-treated cells. KEYWORDS - CLASIFFICATION: analysis;Animals;Anti-Inflammatory Agents;Antineoplastic Agents;Antioxidants;Biology;Biotechnology;Cell Line;Cell Proliferation;chemistry;dietary modulation of carcinogenesis-related pathways;drug effects;Egypt;Galactans;Humans;Macrophages;Mannans;mechanisms of carcinogenesis;metabolism;Mice;Neoplasms;pathology;pharmacology;Plant Gums;prevention & control;Research;secretion;en
dc.description.abstractGuar gum (G) is a simple characterized branched polysaccharide, which is frequently used in food industries. We prepared the gum C-glycosylated derivative (GG), and its sulphated derivative (SGG), aiming to characterize their cancer chemopreventive, and anti-inflammatory properties. Estimation of cancer chemopreventive activity, specifically anti-initiation, including the modulation of carcinogen metabolism and the antioxidant capacity, revealed that GG was a potent anti-initiator, where it inhibited not only the carcinogen activator enzyme, cytochrome P450 1A (CYP1A), but also induced the carcinogen detoxification enzymes glutathione-S-transferases (GSTs), while SGG inhibited both CYP1A and GSTs. SGG was an effective radical scavenger than GG against hydroxyl, peroxyl, and superoxide anion radicals. GG and SGG were found to modulate the macrophage functions into an anti-inflammatory pattern. Thus, both enhanced the macrophage proliferation and phagocytosis of fluorescein isothiocyanate (FITC)-zymosan; however, they also inhibited strongly the nitric oxide generation and tumor necrosis factor-alpha secretion in lipopolysaccharide (LPS)-stimulated RAW macrophage 264.7. Unexpectedly, both GG and SGG dramatically inhibited the binding affinity of FITC-LPS to RAW 264.7, as indicated by flow cytometry analysis. GG and SGG exhibited a significant anti-proliferative activity against human hepatocellular carcinoma cells (Hep G2), and only SGG was specifically cytotoxic for human breast carcinoma cells (MCF-7), but neither was significantly cytotoxic for human lymphoblastic leukemia cells (1301). SGG led to a major disturbance in cell cycle phases of Hep G2 cells as indicated by concomitant arrest in S- and G2/M-phases, a disturbance that was associated with an induced cell death as a result of necrosis, but not apoptosis in both GG- and SGG-treated cells. Taken together, the modified gums could be used as an alternative of G in health food industries to provide cancer prevention in risk populations.en
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T56-4JN72CP-1&_user=1843694&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000055040&_version=1&_urlVersion=0&_userid=1843694&md5=7f5ed225f126c234aaca7338330de6e3en
dc.subjectGuar gumen
dc.subjectCytochrome P450en
dc.subjectCancer chemopreventionen
dc.subjectAnti-initiatingen
dc.subjectAnti-inflammatoryen
dc.subjectAnti-promotingen
dc.subjectMacrophage functionen
dc.subjectApoptosisen
dc.subjectNecrosisen
dc.subject.meshAnimals-
dc.subject.meshAnti-Inflammatory Agents-
dc.subject.meshAntineoplastic Agents-
dc.subject.meshAntioxidants-
dc.subject.meshCell Line-
dc.subject.meshCell Proliferation-
dc.subject.meshGalactans-
dc.subject.meshHumans-
dc.subject.meshMacrophages-
dc.subject.meshMannans-
dc.subject.meshMice-
dc.subject.meshNeoplasms-
dc.subject.meshPlant Gums-
dc.titleCancer chemopreventive and anti-inflammatory activities of chemically modified guar gum.en
dc.typeArticleen
dc.identifier.journalChemico-biological Interactionsen

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