Chemoprevention of mouse urinary bladder carcinogenesis by fermented brown rice and rice bran.

2.50
Hdl Handle:
http://hdl.handle.net/10146/56859
Title:
Chemoprevention of mouse urinary bladder carcinogenesis by fermented brown rice and rice bran.
Authors:
Kuno, Toshiya; Hirose, Yoshinobu; Yamada, Yasuhiro; Hata, Kazuya; Qiang, Sheng Hong; Asano, Nami; Oyama, Takeru; Zhi, Huilan; Iwasaki, Teruaki; Kobayashi, Hiroshi; Mori, Hideki
Abstract:
Fermented brown rice by Aspergillus oryzae (FBRA) has been shown to be a potent anti-carcinogenic compound. Here, we investigated the modifying effects of dietary feeding with a naturally occurring anti-oxidant FBRA on N-butyl-N-(4-hydroxybutyl)-nitrosamine (OH-BBN)-induced urinary bladder carcinogenesis in male ICR mice. Five-week-old male ICR mice were divided into 7 groups, and groups 1-5 were given OH-BBN (500 ppm) in drinking water for 6 weeks starting at 7 weeks of age. Groups 2 and 3 were fed the diet containing 5% and 10% FBRA during the initiation phase, respectively, whereas groups 4 and 5 were fed these diets during the post-initiation phase. Group 6 was given the diet containing 10% FBRA throughout the experiment, and group 7 was kept on the basal diet alone and served as an untreated control. At the end of the study (week 32), the incidences of simple hyperplasia, dysplasia and carcinoma in the bladders of group 1 (OH-BBN alone) were 92%, 49% and 38%, respectively. Those of group 5 (64%, 23% and 10%) and the incidence of carcinoma of group 4 (17%) was significantly less than that of group 1. Furthermore, the multiplicity of simple hyperplasia and carcinoma of group 5 was significantly less than that of group 1. Post-initiation exposure of 10% FBRA significantly decreased the number/nucleus of silver-stained nucleolar organizer region proteins (AgNORs), an index of cell proliferation, in the non-lesional transitional epithelium when compared to that of the control. Our results indicate that FBRA exerts chemopreventive effects against chemically induced urinary bladder carcinogenesis through anti-proliferative mechanisms. FBRA could be a promising chemopreventive agent for human urinary bladder cancer.
Citation:
Oncol. Rep. 2006, 15 (3):533-538
Journal:
Oncology reports
Issue Date:
Mar-2006
URI:
http://hdl.handle.net/10146/56859
PubMed ID:
16465408
Additional Links:
http://www.spandidos-publications.com/or/article.jsp?article_id=or_15_3_533
Type:
Article
Language:
en
Description:
Dietary modulation of cancer & cancer biomarkers Dietary item or component studied: Fermented brown rice by Aspergillus oryzae (FBRA)Outcome studied (cancer or cancer biomarker): urinary bladder cancerStudy type (in vitro, animals, humans): ICR miceMode of exposure (if in vivo): through diet+waterImpact on outcome (including dose-response): In group 1 (OH-BBN alone), the incidence of transitional cell papilloma and carcinoma was 13% (5/39 mice) and 38% (15/39 mice) respectivelyOH-BBN exposure, developed carcinomas (17% in group 4 and 10% in group 5), (P<0.05 and 0.01)Simple hyperplasia in group 5 was significantly smaller than that of group 1 (P<0.01)Dysplasia in group 5 (23%) was significantly lower than that of group 1 (P<0.05).The multiplicity of simple hyperplasia and transitional cell carcinoma of group 5 (0.64±0.47 and 0.10±0.31) was significantly smaller than that of group 1 (0.92±0.27 and 0.62±0.94. KEYWORDS CLASSIFICATION: administration & dosage;Analysis of Variance;Animals;Butylhydroxybutylnitrosamine;chemically induced;chemistry;Cell Proliferation;Chemoprevention;drug effects;Diet;dietary modulation of cancer & cancer biomarkers;dietary modulation of carcinogenesis-related pathways;Hyperplasia;Japan;Male;Mice;Mice,Inbred ICR;Nucleolus Organizer Region;Oryza sativa;pathology;prevention & control;Precancerous Conditions;Research;Silver Staining;toxicity;Urinary Bladder;Urinary Bladder Neoplasms;humans.
ISSN:
1021-335X
Appears in Collections:
Articles with annotation

Full metadata record

DC FieldValue Language
dc.contributor.authorKuno, Toshiya-
dc.contributor.authorHirose, Yoshinobu-
dc.contributor.authorYamada, Yasuhiro-
dc.contributor.authorHata, Kazuya-
dc.contributor.authorQiang, Sheng Hong-
dc.contributor.authorAsano, Nami-
dc.contributor.authorOyama, Takeru-
dc.contributor.authorZhi, Huilan-
dc.contributor.authorIwasaki, Teruaki-
dc.contributor.authorKobayashi, Hiroshi-
dc.contributor.authorMori, Hideki-
dc.date.accessioned2009-03-23T10:02:40Z-
dc.date.available2009-03-23T10:02:40Z-
dc.date.issued2006-03-
dc.identifier.citationOncol. Rep. 2006, 15 (3):533-538en
dc.identifier.issn1021-335X-
dc.identifier.pmid16465408-
dc.identifier.urihttp://hdl.handle.net/10146/56859-
dc.descriptionDietary modulation of cancer & cancer biomarkers Dietary item or component studied: Fermented brown rice by Aspergillus oryzae (FBRA)Outcome studied (cancer or cancer biomarker): urinary bladder cancerStudy type (in vitro, animals, humans): ICR miceMode of exposure (if in vivo): through diet+waterImpact on outcome (including dose-response): In group 1 (OH-BBN alone), the incidence of transitional cell papilloma and carcinoma was 13% (5/39 mice) and 38% (15/39 mice) respectivelyOH-BBN exposure, developed carcinomas (17% in group 4 and 10% in group 5), (P<0.05 and 0.01)Simple hyperplasia in group 5 was significantly smaller than that of group 1 (P<0.01)Dysplasia in group 5 (23%) was significantly lower than that of group 1 (P<0.05).The multiplicity of simple hyperplasia and transitional cell carcinoma of group 5 (0.64±0.47 and 0.10±0.31) was significantly smaller than that of group 1 (0.92±0.27 and 0.62±0.94. KEYWORDS CLASSIFICATION: administration & dosage;Analysis of Variance;Animals;Butylhydroxybutylnitrosamine;chemically induced;chemistry;Cell Proliferation;Chemoprevention;drug effects;Diet;dietary modulation of cancer & cancer biomarkers;dietary modulation of carcinogenesis-related pathways;Hyperplasia;Japan;Male;Mice;Mice,Inbred ICR;Nucleolus Organizer Region;Oryza sativa;pathology;prevention & control;Precancerous Conditions;Research;Silver Staining;toxicity;Urinary Bladder;Urinary Bladder Neoplasms;humans.en
dc.description.abstractFermented brown rice by Aspergillus oryzae (FBRA) has been shown to be a potent anti-carcinogenic compound. Here, we investigated the modifying effects of dietary feeding with a naturally occurring anti-oxidant FBRA on N-butyl-N-(4-hydroxybutyl)-nitrosamine (OH-BBN)-induced urinary bladder carcinogenesis in male ICR mice. Five-week-old male ICR mice were divided into 7 groups, and groups 1-5 were given OH-BBN (500 ppm) in drinking water for 6 weeks starting at 7 weeks of age. Groups 2 and 3 were fed the diet containing 5% and 10% FBRA during the initiation phase, respectively, whereas groups 4 and 5 were fed these diets during the post-initiation phase. Group 6 was given the diet containing 10% FBRA throughout the experiment, and group 7 was kept on the basal diet alone and served as an untreated control. At the end of the study (week 32), the incidences of simple hyperplasia, dysplasia and carcinoma in the bladders of group 1 (OH-BBN alone) were 92%, 49% and 38%, respectively. Those of group 5 (64%, 23% and 10%) and the incidence of carcinoma of group 4 (17%) was significantly less than that of group 1. Furthermore, the multiplicity of simple hyperplasia and carcinoma of group 5 was significantly less than that of group 1. Post-initiation exposure of 10% FBRA significantly decreased the number/nucleus of silver-stained nucleolar organizer region proteins (AgNORs), an index of cell proliferation, in the non-lesional transitional epithelium when compared to that of the control. Our results indicate that FBRA exerts chemopreventive effects against chemically induced urinary bladder carcinogenesis through anti-proliferative mechanisms. FBRA could be a promising chemopreventive agent for human urinary bladder cancer.en
dc.language.isoenen
dc.relation.urlhttp://www.spandidos-publications.com/or/article.jsp?article_id=or_15_3_533en
dc.subjectFermented brown riceen
dc.subjectRice branen
dc.subjectUrinary bladder canceren
dc.subject.meshAnalysis of Variance-
dc.subject.meshAnimals-
dc.subject.meshButylhydroxybutylnitrosamine-
dc.subject.meshCell Proliferation-
dc.subject.meshDiet-
dc.subject.meshHyperplasia-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred ICR-
dc.subject.meshNucleolus Organizer Region-
dc.subject.meshOryza sativa-
dc.subject.meshPrecancerous Conditions-
dc.subject.meshSilver Staining-
dc.subject.meshUrinary Bladder-
dc.subject.meshUrinary Bladder Neoplasms-
dc.titleChemoprevention of mouse urinary bladder carcinogenesis by fermented brown rice and rice bran.en
dc.typeArticleen
dc.identifier.journalOncology reportsen

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